SEC-MS: Recombinant human serum albumin (rHSA)

SEC: Polyvinyl Alcohol (PVA)

SEC: Polyvinyl Acetate (PVAc)

SEC: Polyvinylpyrrolidone (PVP)

SEC: Monoclonal antibody (mAb)

SEC: Monoclonal antibody (mAb)

SEC: Hemoglobin

SEC: Polyethylene Glycol

SEC: Polystyrene

SEC: 20bp DNA Ladder

SEC: Glycosaminoglycans (GAGs)

SEC separation: Thyroglobulin (Tg)

SEC separation for proteins (column connection)

BSA (Bovine Serum Albumin) Monomer, Dimer

LC-MS: Flavonoids

LC-MS: Flavonoids

LC-MS: Flavonoids

LC-MS: Amino acids standard

LC-MS: Taxifolin

LC-MS: 20 standard amino acids

LC-MS: Thyroid hormone

LC-MS: Kynurenine / Tryptophan Ratio (KTR) of Human serum

LC-MS: Kynurenine / Tryptophan Ratio (KTR) of Human serum

LC-MS: Kynurenine / Tryptophan Ratio (KTR)

LC-MS: Orexin A and B

LC-MS: Orexin A and B

LC-MS: Orexin A and B

LC-MS: Kynurenine metabolism pathway

LC-MS: Amino acids standard

LC-MS: Nucleosides and bases (mobile phase pH)

LC-MS: Antipsychotics

LC-MS: Antipsychotics

LC-MS: Nucleosides and bases

LC-MS: Amino acids standard

Antibiotic (vancomycin)

LC-MS: SAH and SAM

LC-MS: antimigraines (selective serotonin agonists)

LC-MS: antimigraines (selective serotonin agonists)

LC-MS: Acidic compound (mobile phase pH)

Paraben isomers

LC-MS: antimigraines (selective serotonin agonists)

LC-MS: Insulin (Mobile phase pH)

LC-MS: Amyloid beta-peptide [Human,1-40] (Mobile phase pH)

LC-MS: Amyloid beta-peptide [Human,1-40] (alkali conditions)

LC-MS: Basic compound (mobile phase pH)

LC-MS: Alkaloids (alkali conditions)

Tricyclic antidepressants

Soybean protein

LC-MS: sugar alcohols

LC-MS: mono and disaccharides

Lipid A (Salmonella minnesota Re595)

Recombinant human serum albumin (rHSA)

Recombinant and biosynthetic human serum albumin (HSA)

Recombinant human serum albumin (rHSA)

LC-MS: retro-dipeptide isomers

LC-MS, Biochemistry/Metabolism, Amino Acids and Peptides

LC-MS: choline metabolites and related compounds

LC-MS: isoproterenol

LC-MS: carnitine related compounds

LC-MS: Amino acids in human serum (HClO4)

LC-MS: Amino acids in human serum (SSA)

LC-MS: dipeptides

LC-MS: Low-calorie sweeteners

LC-MS: Fungicides for citrus fruits

Efficient chromatographic separation of intact proteins derivatized with a fluorogenic reagent for proteomics analysis

LC-MS: Epinephrine and metanephrine

LC-MS: Synthetic polyamines

LC-MS: Biogenic polyamines

LC-MS: Method for simultaneous analysis of functional ingredients in rice bran oil

LC-MS/MS: Acetylcholine and d4-acetylcholine

LC-MS: Neuroblastoma related compounds

LC-MS: Neurotransmitters (2)

LC-MS: Neurotransmitters (1)

LC-MS: Catecholamines

LC-MS: Polyoxin D

LC-MS: 20 Amino acids and 5 isotopes (standard)

LC-MS: Adenosine phosphates (AMP,ADP, ATP)

LC-MS: 39 Amino acids (standard)

LC-MS: Aspartame and diastereomers

LC-MS: Microcystins

LC-MS: Nicotine related compounds (3)

LC-MS: Nicotine related compounds (2)

LC-MS: Nicotine related compounds (1)

LC-MS: 20 Standard Amino Acids

LC-MS: 20 Standard Amino Acids

LC-MS: Ascorbic acid related compounds

Propagermanium

LC-MS: biogenic amines and precursor amino acids (3)

LC-MS: biogenic amines and precursor amino acids (2)

LC-MS: biogenic amines and precursor amino acids

LC-MS: Amino acids in serum (SSA)

LC-MS: Amino acid in human serum (HClO4)

LC-MS: Photo Acid Generators

LC-MS: 1min High Throughput Analysis Amino Acids

LC-MS: Homocysteine and relative compounds (3)

LC-QTOF/MS: Acyl carnitine

LC-MS: Homocysteine and relative compounds (2)

Homocysteine and relative compounds (recommended)

LC-MS: alpha-Disubstituted amino acids (2)

LC-MS: alpha-Disubstituted amino acids (recommended)

LC-MS: 20 standard amino acids (recommended)

LC-MS: Amino acids in yogurt

Oxoacids of arsenic

Sodium Halides

LC-MS: Lysinoalanine related compounds

LC-MS: Lysinoalanine related compounds

LC-MS: Amino acids in beer

LC-MS: Amino acids in white wine

LC-MS: Amino acids in green tea

LC-MS: Amino acids in bouillon of dried bonito

LC-MS: Amino acids in fermented milk drink

LC-MS: Amino acids in milk

LC-MS: Bisphosphonates (bone resorption inhibitors)

Oxoacids of notrogen

Oxoacids of chlorine

Oxoacid of iodine

Sodium Halides

High Throughput Reliable Quantitation of 25-hydroxyvitamin D in Serum by Offline Sample Preparation and a LC-MS/MS Instrument

LC-MS: Amino acids and water-soluble vitamins in carbonated beverage

LC-MS: Cysteic acid related compounds

A high throughput solid phase extraction method for vitamin B7 (biotin) from pooled human serum prior to mixed mode LC-MS/MS

LC-MS: Leucine isomers

LC-MS: Leucine isomers

LC-MS: Valine isomers (2)

LC-MS: Valine isomers

LC-MS: Threonine isomers

LC-MS: Histidine derivatives

LC-MS: Alanine isomers

LC-MS: Glutamic acid and GABA isomers

Batch-to-Batch Reproducibility (Intrada Amino Acid)

LC-MS: 20 standard amino acids (high resolution)

LC-MS: 20 standard amino acids (recommended)

Use of the Intrada Amino Acid column along with tandem mass spectrometry allows for separation and detection of all 20 standard amino acids in under 20 minutes. This column enables the separation of isobaric amino acids such as leucine and isoleucine.

The Intrada Amino Acid column is used to separate amino acids present within soy sauce

The Intrada Amino Acid column separates all 20 amino acids in under 15 minutes without requiring tedious pre or post labeling methods

The Intrada Amino Acid column sucessfully separates 55 amino acids and related compounds without requiring derivatization

The Unison UK-Amino column successfully separates adenosine and inosine, which are very similar in structure.

The Scherzo SM-C18 and Unison UK-Amino columns successfully separate the small, polar ascorbic acid related compounds in normal phase mode.

The Scherzo SS-C18 column provides better retention of Shikimic Acid than a standard C18 column.

The Unison UK-Phenyl column separates surfactants of different lengths better than a standard C18 column.

The Unison UK-Phenyl column effectively separates fluoroamphetamine and fluoromethamphetamine isomers with identical mass.

The Unison UK-Phenyl column is effectively able to separate triazolam and etizolam, which are very similar in structure.

Cadenza CW-C18, Unison UK-C18, and Unison UK-C8 are all able to resolve the very polar and charged Acetyl CoA and CoA, differing only in an acetyl group.

Oligopeptides (angiotensins)

Oligopeptides (angiotensins)

Oligopeptides (angiotensins)

The Scherzo SS-C18 column is effectively able to retain and resolve polar dipeptides.

The Cadenza HS-C18 column is able to retain organotin compounds with nice, sharp peaks.

The Scherzo SM-C18 and Scherzo SS-C18 column resolve very polar carboxylic acids that normally co-elute on a standard C18 column (Unison UK-C18).

The Scherzo SS-C18 column retains Prostaglandins the most, followed by Scherzo SM-C18 and Scherzo SW-C18.

Under neutral pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

Under acidic pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

On the Unison UK-C18 column, tetrahydrofuran gives better resolution of Prostaglandins than acetonitrile and acetone.

Acetonitrile and methanol have opposite retention properties in the separation of prostaglandins on the Unison UK-C18 column.

The Scherzo SM-C18 column is able to retain a highly polar compound (multiple phosphate groups) better than a standard C18 column.

The Scherzo SW-C18 column is able to retain compounds with multiple amine functionalities.

The Scherzo SS-C18 column is able to retain compounds with multiple amine functionalities.

The Scherzo SM-C18 column successfully resolves nucleotide triphosphates of similar structure.

The Cadenza CD-C18 HT 3 micron column shows comparable resolution to a sub-2 micron column, with much lower pressure.

The Cadenza CD-C18 HT 3 micron column shows better resolution between isopropylparaben and propylparaben than a sub-2 micron column, and the CD-C18 HT column also shows a higher plate count.

The Cadenza CD-C18 HT 3 micron column shows lower pressure, higher plate count, and higher resolution than a sub-2 micron column.

Tadalafil, Sildenafil, and Vardenafil are all polar small molecules that are easily separated on the Unison UK-C18 HT column. Not only are the slight structural differences between Sildenafil and Vardenafil recognized by the column, but it also separates all three compounds within 1.5 minutes.

Tannic acid

The Scherzo SM-C18 column can separate these two molecules, which would normally coelute on a standard C18 column.

The Scherzo SM-C18 and SS-C18 show excellent resolution of these small molecules, which normally coelute on a standard C18 column.

The Scherzo SS-C18 column shows improved resolution of arsenobetaine compared to a standard C18 column. The Scherzo SM-C18 and the Scherzo SW-C18 show similar retention of this zwitterion as the standard Unison UK-C18 column.

The Scherzo SM-C18 and the Scherzo SW-C18 show similar retention of these small molecules as the standard Unison UK-C18. However, the Scherzo SS-C18 shows the best resolution between these three compounds.

The Scherzo family columns can separate this glycopeptide with varying retention. The Scherzo SW-C18 has the least retention, followed by the Scherzo SM-C18, followed by the Scherzo SS-C18 which has the most retention.

The Scherzo SM-C18 and SW-C18 columns can separate this glycopeptide with varying retention. The Scherzo SM-C18 has less retention while the Scherzo SW-C18 has more retention.

The Scherzo SS-C18 column retains and separates glutamic acid and GABA better than a conventional C18 column

LC-MS/MS Application for non-opioid drugs in human plasma

Metabolites of toluene, xylene and stylene

Blowing agents (OBSH, TSSC) for plastic materials

Small, polar molecules like neurotransmitters are difficult to retain on a conventional C18 column like Unison UK-C18. Scherzo SS-C18 can retain these compounds much better than a standard Unison UK-C18 column, as well as resolve the peaks better. Conditions compatible with LC-MS

Unison UK-C18 column can separate out the small, polar molecule components of coffee bean extract due to the lower density of C18 ligands. The column can generate 219 peaks, which shows all of the components of the extract.

Both Unison UK-C18 and Scherzo SW-C18 can separate out components of coffee extracts, but Scherzo SW-C18 can resolve the peaks of caffeine and ferulic acid better.

The highly charged phosphate groups make separation of AMP, ADP, and ATP difficult on a conventional C18 column. Scherzo SW-C18 is able to elute these compounds effectively, in both ammonium acetate and ammonium phosphate buffers.

Both Scherzo SM-C18 and Scherzo SW-C18 can separate an acidic compound (Ibuprofen) and a basic compound (Chlorpheniramine) at the same time. Scherzo SW-C18 works better under gradient elution, whereas Scherzo SM-C18 works better under isocratic elution.

All of the columns listed can recognize the structural difference between these two isomers, and resolve them into two separate peaks.

Hyaluronic Acid

A deep gradient on the Presto FF-C18 column results in a nice, sharp peak for hyaluronic acid. More shallow gradients result in broader peaks that can better separate out conformational differences or MW recogntion.

Both Presto FF-C18 and Intrada WP-RP can elute this large protein. Intrada WP-RP shows sharper peaks, meaning it can better recognize its structural components than Presto FF-C18.

Peptide mapping using LC-FTMS

Cytochrome c

Serotonin related compounds

Histamine related compounds

Neurotransmitters

Oligonucleotides

Acetaminophen and metabolite

Acetaminophen and metabolite

LC-MS/MS Application for Naturally Occurring Anti-inflammatory Pyroglutamyl Peptide in Japanese Rice Wine

Ultra-high throughput LC-MS/MS analysis of drugs in blood plasma

Amyloid beta-protein

siRNA

Basic pharmaceutical compounds

Zwitterionic compounds

Zwitterionic compounds

Catecholamines

Endosulfan isomers

Endosulfan isomers

Endosulfan isomers

LC-ICP-MS analysis of Cr(III) and Cr(VI)

LC-MS analysis of phosphorus-containing amino acid type herbicides and their metabolites

Dextran Sulfate

Penicillin Antibiotics

Collagenpeptide

Farnesene isomers

LC-MS/MS analysis of chloramphenicol (CP)

LC-MS/MS analysis of [leuco]malachite green (MG, LMG)

LC-MS/MS analysis of clenbuterol (CBR)

LC/MS analysis of nitrofurans metabolites (AOZ, AMOZ, AHD)

LC/MS analysis of perfluorinated organic compounds (PFC's) in seawater

The effect of TFA modifier on peptide mapping

Exploring different C18 stationary phases for metabolites profiling

Hyaluronic acid, chondrotin sulfuric acid

Surfactants

Simulatneous HPLC/LT-ELSD Analysis of Organic and Phenolic Acids

Simulatneous HPLC/LT-ELSD Analysis of Water Soluble and Fat Soluble Vitamins

Simultaneous HPLC/LT-ELSD Analysis of Polyols, Mono-,Di- and Oligosaccarides

Peptide Mapping (50 C formic acid)

LC-MS/MS Application: Simultaneous determination of phytotoxins in urine

Protein separation on non-porous ODS column

Protein separation on non-porous ODS column

Scherzo is able to separate mandelic acid and dopamine, which coelute on a conventional C18 column (Unison UK-C18). This is possible because the ionic ligands recognize the small structural differences between the two compounds.

Catecholamine metabolites

Cold medicine compounds

Cold medicine compounds

Theaflavins

The effect of gradient slope and column length for alpha-lactalbumin separation

The effect of gradient slope and column length for cytochrome c separation

The effect of gradient slope and column length for thyroglobulin separation

The effect of gradient slope and column length for lectin separation

The effect of gradient slope and column length for ovalbumin separation

The effect of gradient slope and column length for BSA separation

The effect of gradient slope for insulin separation

The effect of gradient slope for angiotensin II serparation

Coumarins

The effect of gradient slope and column length for HSA serpation

Gradient mixer volume affecting HSA separation

Polyethylene glycol (PEG)