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Technical Information   Posters   Papers

Application #:
TI0749
Type:
Application Note
Categories:
Vitamins
Clinical Diagnostics
Compounds:
Vitamin B7 (biotin)
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

A high throughput solid phase extraction method for vitamin B7 (biotin) from pooled human serum prior to mixed mode LC-MS/MS

Application #:
TI0737
Type:
Application Note
Categories:
LC-MS
Amino Acids and Peptides
Compounds:
Standard Amino Acids
Column(s):
Intrada Amino Acid (50 x 3.0mm)

Use of the Intrada Amino Acid column along with tandem mass spectrometry allows for separation and detection of all 20 standard amino acids in under 20 minutes. This column enables the separation of isobaric amino acids such as leucine and isoleucine.

Application #:
TI0736
Type:
Application Note
Categories:
LC-MS
Amino Acids and Peptides
Food Chemistry
Compounds:
Amino acids
Column(s):
Intrada Amino Acid (50 x 3.0mm)

The Intrada Amino Acid column is used to separate amino acids present within soy sauce

Application #:
TI0735
Type:
Application Note
Categories:
LC-MS
Amino Acids and Peptides
Compounds:
20 standard amino acids
Column(s):
Intrada Amino Acid (50 x 3.0mm)

The Intrada Amino Acid column separates all 20 amino acids in under 15 minutes without requiring tedious pre or post labeling methods

Application #:
TI0734
Type:
Application Note
Categories:
LC-MS
Amino Acids and Peptides
Compounds:
55 amino acids and related compounds
Column(s):
Intrada Amino Acid (50 x 3.0mm)

The Intrada Amino Acid column sucessfully separates 55 amino acids and related compounds without requiring derivatization

Application #:
TI0733
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
adenosine
hypoxanthine
inosine
Column(s):
Unison UK-Amino (75 x 3.0mm)

The Unison UK-Amino column successfully separates adenosine and inosine, which are very similar in structure.

Application #:
TI0732
Type:
Application Note
Categories:
Biochemistry/Metabolism
Vitamins
Industrial Chemical
Food Chemistry
Compounds:
"isoascorbic acid (erythorbic acid)
L-ascorbic acid
L-ascorbic acid 2-glucoside"
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-Amino (75 x 3.0mm)

The Scherzo SM-C18 and Unison UK-Amino columns successfully separate the small, polar ascorbic acid related compounds in normal phase mode.

Application #:
TI0731
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
shikimic acid
L-tyrosine
L-phenylalanine
L-tryptophan
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

The Scherzo SS-C18 column provides better retention of Shikimic Acid than a standard C18 column.

Application #:
TI0730
Type:
Application Note
Categories:
Industrial Chemical
Compounds:
sodium alkylbenzenesulfonates (including branched structure)
Column(s):
Unison UK-C18 (150 x 3.0mm)
Unison UK-Phenyl (150 x 3.0mm)

The Unison UK-Phenyl column separates surfactants of different lengths better than a standard C18 column.

Application #:
TI0729
Type:
Application Note
Categories:
LC-MS
Pharmaceuticals and Cosmetics
Compounds:
4-fluoroamphetamine 4-FMP
3-fluoroamphetamine 3-FMP
2-fluoroamphetamine 2-FMP
4-fluoromethamphetamine N-methyl-4-FMP
3-fluoromethamphetamine N-methyl-3-FMP
2-fluoromethamphetamine N-methyl-2-FMP
Column(s):
Unison UK-Phenyl (150 x 3.0mm)

The Unison UK-Phenyl column effectively separates fluoroamphetamine and fluoromethamphetamine isomers with identical mass.

Application #:
TI0728
Type:
Application Note
Categories:
LC-MS
Pharmaceuticals and Cosmetics
Compounds:
Triazolam
etizolam
8OH-etizolam
1OH-etizolam
4OH-triazolam
1OH-triazolam
Column(s):
Unison UK-Phenyl (150 x 2.0mm)

The Unison UK-Phenyl column is effectively able to separate triazolam and etizolam, which are very similar in structure.

Application #:
TI0727
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Coenzyme A (CoA)
acetyl coenzyme (Acetyl-CoA)
Column(s):
Cadenza CW-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)

Cadenza CW-C18, Unison UK-C18, and Unison UK-C8 are all able to resolve the very polar and charged Acetyl CoA and CoA, differing only in an acetyl group.

Application #:
TI0722
Type:
Application Note
Categories:
Amino Acids and Peptides
Compounds:
"L-cystine (Cys)
N-(L-alpha-glutamyl) -L-glutamic acid (Glu-Glu)
N-glycyl-L- proline (Gly-Pro)
L-anserine
L-carnosine
N-L-histidyl-L-leucine (His-Leu)"
Column(s):
Scherzo SS-C18 (150 x 3.0mm)

The Scherzo SS-C18 column is effectively able to retain and resolve polar dipeptides.

Application #:
TI0721
Type:
Application Note
Categories:
Industrial Chemical
Compounds:
Azocyclotin
cyhexatin
Column(s):
Cadenza HS-C18 (150 x 3.0mm)

The Cadenza HS-C18 column is able to retain organotin compounds with nice, sharp peaks.

Application #:
TI0720
Type:
Application Note
Categories:
Biochemistry/Metabolism
Botanicals
Compounds:
Shikimic acid
malic acid
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

The Scherzo SM-C18 and Scherzo SS-C18 column resolve very polar carboxylic acids that normally co-elute on a standard C18 column (Unison UK-C18).

Application #:
TI0719
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)

The Scherzo SS-C18 column retains Prostaglandins the most, followed by Scherzo SM-C18 and Scherzo SW-C18.

Application #:
TI0718
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)
Unison UK-Phenyl (150 x 3.0mm)

Under neutral pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

Application #:
TI0717
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)
Unison UK-Phenyl (150 x 3.0mm)

Under acidic pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

Application #:
TI0716
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Unison UK-C18 (150 x 3.0mm)

On the Unison UK-C18 column, tetrahydrofuran gives better resolution of Prostaglandins than acetonitrile and acetone.

Application #:
TI0715
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Unison UK-C18 (150 x 3.0mm)

Acetonitrile and methanol have opposite retention properties in the separation of prostaglandins on the Unison UK-C18 column.

Application #:
TI0714
Type:
Application Note
Categories:
Botanicals
Food Chemistry
Compounds:
inositol hexaphosphate (phytic acid )
Column(s):
Scherzo SM-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)

The Scherzo SM-C18 column is able to retain a highly polar compound (multiple phosphate groups) better than a standard C18 column.

Application #:
TI0713
Type:
Application Note
Categories:
Industrial chemical
Compounds:
"ethylenediamine
diethylenetriamine
triethylenetetramine (including branched structures)
tetraethylenepentamine (including branched structures)"
Column(s):
Scherzo SW-C18 (150 x 3.0mm)

The Scherzo SW-C18 column is able to retain compounds with multiple amine functionalities.

Application #:
TI0712
Type:
Application Note
Categories:
Industrial chemical
Compounds:
ethylenediamine
diethylenetriamine
Column(s):
Scherzo SS-C18 (150 x 3.0mm)

The Scherzo SS-C18 column is able to retain compounds with multiple amine functionalities.

Application #:
TI0710
Type:
Application Note
Categories:
Biochemistry and Metabolism
Compounds:
CTP
UTP
TTP
dCTP
GTP
ATP
dGTP
dTTP
dATP
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

The Scherzo SM-C18 column successfully resolves nucleotide triphosphates of similar structure.

Application #:
TI0709
Type:
Application Note
Categories:
Pharmaceutical and Cosmetics
DNA related compounds
Column Performance
Compounds:
uracil
isopropylparaben
propylparaben
butylparaben
Column(s):
Cadenza CD-C18 (100 x 4.6mm)

The Cadenza CD-C18 HT 3 micron column shows comparable resolution to a sub-2 micron column, with much lower pressure.

Application #:
TI0708
Type:
Application Note
Categories:
Pharmaceutical and Cosmetics
DNA related compounds
Column Performance
Compounds:
uracil
isopropylparaben
propylparaben
butylparaben
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

The Cadenza CD-C18 HT 3 micron column shows better resolution between isopropylparaben and propylparaben than a sub-2 micron column, and the CD-C18 HT column also shows a higher plate count.

Application #:
TI0707
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
DNA related compounds
Column Performance
Compounds:
uracil
isopropylparaben
propylparaben
butylparaben
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

The Cadenza CD-C18 HT 3 micron column shows lower pressure, higher plate count, and higher resolution than a sub-2 micron column.

Application #:
TI0706
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Compounds:
Tadalafil
Sildenafil and Vardenafil
Column(s):
Unison UK-C18 (75 x 2.0mm)

Tadalafil, Sildenafil, and Vardenafil are all polar small molecules that are easily separated on the Unison UK-C18 HT column. Not only are the slight structural differences between Sildenafil and Vardenafil recognized by the column, but it also separates all three compounds within 1.5 minutes.

Application #:
TI0704
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Spermine and spermidine
Column(s):
Scherzo SM-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)

The Scherzo SM-C18 column can separate these two molecules, which would normally coelute on a standard C18 column.

Application #:
TI0703
Type:
Application Note
Categories:
Industrial Chemical
Compounds:
Formic acid and acetic acid
Column(s):
Scherzo SW-C18 (250 x 3.0mm)
Scherzo SM-C18 (250 x 3.0mm)
Scherzo SS-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

The Scherzo SM-C18 and SS-C18 show excellent resolution of these small molecules, which normally coelute on a standard C18 column.

Application #:
TI0702
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
Arsenobetaine
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

The Scherzo SS-C18 column shows improved resolution of arsenobetaine compared to a standard C18 column. The Scherzo SM-C18 and the Scherzo SW-C18 show similar retention of this zwitterion as the standard Unison UK-C18 column.

Application #:
TI0701
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Botanicals
Biochemistry/Metabolism
Compounds:
Allantoin
allantoic acid and uric acid
Column(s):
Scherzo SW-C18 (250 x 3.0mm)
Scherzo SM-C18 (250 x 3.0mm)
Scherzo SS-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

The Scherzo SM-C18 and the Scherzo SW-C18 show similar retention of these small molecules as the standard Unison UK-C18. However, the Scherzo SS-C18 shows the best resolution between these three compounds.

Application #:
TI0700
Type:
Application Note
Categories:
Saccharides
Amino Acids and Peptides
Compounds:
Sialylglycopeptide
Column(s):
Scherzo SW-C18 (250 x 3.0mm)
Scherzo SM-C18 (250 x 3.0mm)
Scherzo SS-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

The Scherzo family columns can separate this glycopeptide with varying retention. The Scherzo SW-C18 has the least retention, followed by the Scherzo SM-C18, followed by the Scherzo SS-C18 which has the most retention.

Application #:
TI0699
Type:
Application Note
Categories:
Saccharides
Amino Acids and Peptides
Compounds:
Sialylglycopeptide
Column(s):
Scherzo SW-C18 (250 x 3.0mm)
Scherzo SM-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

The Scherzo SM-C18 and SW-C18 columns can separate this glycopeptide with varying retention. The Scherzo SM-C18 has less retention while the Scherzo SW-C18 has more retention.

Application #:
TI0698
Type:
Application Note
Categories:
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
L-glutamic acid
gamma-aminobutyric acid (GABA)
Column(s):
Scherzo SS-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

The Scherzo SS-C18 column retains and separates glutamic acid and GABA better than a conventional C18 column

Application #:
TI0694
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
"L-glutamic acid
gammaaminobutyric acid (GABA)
3
4-dihydroxyL-phenylalanine (L-DOPA)
5-hydroxyindole- 3-acetic acid (5-HIAA)
L-tyrosine
L-noradrenaline (L-norepinephrine)
acetylcholine chloride
L-adrenaline (L-epinephrine)
5-hydroxyL-tryptophan
L-phenylalanine
dopamine
L-tryptophan
serotonin"
Column(s):
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Small, polar molecules like neurotransmitters are difficult to retain on a conventional C18 column like Unison UK-C18. Scherzo SS-C18 can retain these compounds much better than a standard Unison UK-C18 column, as well as resolve the peaks better. Conditions compatible with LC-MS

Application #:
TI0693
Type:
Application Note
Categories:
Food Chemistry
Compounds:
quinic acid
hydroxyhydroquinone
3
4-dihydroxyhydrocinnamic acid
caffeine
caffeic acid
p-coumaric acid
ferulic acid
isoferulic acid
Column(s):
Unison UK-C18 (250 x 4.6mm)

Unison UK-C18 column can separate out the small, polar molecule components of coffee bean extract due to the lower density of C18 ligands. The column can generate 219 peaks, which shows all of the components of the extract.

Application #:
TI0692
Type:
Application Note
Categories:
Food Chemistry
Compounds:
hydroxyhydroquinone
3
4-dihydroxyhydrocinnamic acid
caffeic acid
p-coumaric acid
caffeine
ferulic acid
isoferulic acid
Column(s):
Scherzo SW-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

Both Unison UK-C18 and Scherzo SW-C18 can separate out components of coffee extracts, but Scherzo SW-C18 can resolve the peaks of caffeine and ferulic acid better.

Application #:
TI0691
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
AMP
ADP
ATP
Column(s):
Scherzo SW-C18 (150 x 3.0mm)

The highly charged phosphate groups make separation of AMP, ADP, and ATP difficult on a conventional C18 column. Scherzo SW-C18 is able to elute these compounds effectively, in both ammonium acetate and ammonium phosphate buffers.

Application #:
TI0690
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Compounds:
Ibuprofen
Chlorpheniramine
Column(s):
Scherzo SW-C18 (150 x 4.6mm)
Scherzo SM-C18 (150 x 4.6mm)

Both Scherzo SM-C18 and Scherzo SW-C18 can separate an acidic compound (Ibuprofen) and a basic compound (Chlorpheniramine) at the same time. Scherzo SW-C18 works better under gradient elution, whereas Scherzo SM-C18 works better under isocratic elution.

Application #:
TI0689
Type:
Application Note
Categories:
Botanicals
Pharmaceuticals and Cosmetics
Compounds:
trans-resveratrol
cis-resveratrol
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Presto FF-C18 (150 x 3.0mm)

All of the columns listed can recognize the structural difference between these two isomers, and resolve them into two separate peaks.

Application #:
TI0687
Type:
Application Note
Categories:
Polysaccharides
Compounds:
Hyaluronic acid
Column(s):
Presto FF-C18 (250 x 4.6mm)

A deep gradient on the Presto FF-C18 column results in a nice, sharp peak for hyaluronic acid. More shallow gradients result in broader peaks that can better separate out conformational differences or MW recogntion.

Application #:
TI0686
Type:
Application Note
Categories:
Proteins
Industrial Chemical
Pharmaceuticals and Cosmetics
Compounds:
collagen from pig skin
Column(s):
Intrada WP-RP (250 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

Both Presto FF-C18 and Intrada WP-RP can elute this large protein. Intrada WP-RP shows sharper peaks, meaning it can better recognize its structural components than Presto FF-C18.

Application #:
TI0685
Type:
Application Note
Categories:
Proteins
Amino Acids and Peptides
LC-MS
Compounds:
tryptic digest of alpha-casein
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Presto FF-C18 (150 x 3.0mm)

Peptide mapping using LC-FTMS

Application #:
TI0684
Type:
Application Note
Categories:
Biochemistry/Metabolism
Botanicals
Proteins
Compounds:
cytochrome c (horse heart
12.4 kDa)
Column(s):
Presto FF-C18 (150 x 3.0mm)

Cytochrome c

Application #:
TI0683
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
"5-hydroxy-L-tryptophan
serotonin
L-tryptophan
5-hydroxyindole -3-acetic acid (5-HIAA)"
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)

Serotonin related compounds

Application #:
TI0682
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
"L-histidine
N-methyl-4- imidazoleacetic acid
4-imidazoleacetic acid
N-methylhistamine
histamine"
Column(s):
Scherzo SS-C18 (150 x 3.0mm)

Histamine related compounds

Application #:
TI0681
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
L-glutamic acid (Glu)
4-aminobutyric acid (GABA)
L-DOPA
acetylcholine chloride
L-adrenaline
L-noradrenaline
dopamine
Column(s):
Scherzo SS-C18 (150 x 3.0mm)
Scherzo SS-C18 (250 x 3.0mm)

Neurotransmitters

Application #:
TI0680
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
oligothymidylic acid d(pT) ammonium salt
Column(s):
Scherzo SW-C18 (150 x 3.0mm)

Oligonucleotides

Application #:
TI0679
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Compounds:
" acetaminophen
N-acetyl-4-benzoquinone imine"
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Acetaminophen and metabolite

Application #:
TI0678
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Compounds:
" acetaminophen
N-acetyl-4-benzoquinone imine"
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Acetaminophen and metabolite

Application #:
TI0676
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
LC-MS
Compounds:
nifedipine
warfarin
diclofenac
2-amino-5-chlorobenzophenone
Column(s):
Cadenza CD-C18 (50 x 2.0mm)

Ultra-high throughput LC-MS/MS analysis of drugs in blood plasma

Application #:
TI0675
Type:
Application Note
Categories:
Amino acids and peptides
Proteins
Compounds:
amyloid -protein
Column(s):
Intrada WP-RP (100 x 4.6mm)
Cadenza CW-C18 (100 x 4.6mm)
Presto FF-C18 (100 x 4.6mm)

Amyloid beta-protein

Application #:
TI0673
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
siRNA Universal Negative Control
Control siRNA
Negative Control
Control siRNA duplex (Human cdk1)
Control siRNA duplex (Human beta-actin)
Column(s):
Scherzo SW-C18 (150 x 3.0mm)

siRNA

Application #:
TI0672
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Compounds:
phenylephrine
N-acetylprocainamide
procaine
naphazoline
Column(s):
Scherzo SW-C18 (150 x 3.0mm)

Basic pharmaceutical compounds

Application #:
TI0671
Type:
Application Note
Categories:
Biochemistry and Metabolism
Amino Acids and Peptides
Compounds:
creatine phosphate
betaine
L-carnitine
creatine
L-theanine
L-orthinine
Column(s):
Scherzo SS-C18 (150 x 3.0mm)

Zwitterionic compounds

Application #:
TI0670
Type:
Application Note
Categories:
Biochemistry and Metabolism
Amino Acids and Peptides
Compounds:
creatine phosphate
betaine
L-carnitine
creatine
L-theanine
Column(s):
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Zwitterionic compounds

Application #:
TI0669
Type:
Application Note
Categories:
Biochemistry/Metabolism
Pharmaceuticals and Cosmetics
Compounds:
gamma-aminobutyric acid (GABA)
D
L-noradrenaline ((D
L-norepinephrine)
D
L-adrenaline (D
L-epinephrine)
3
4-dihydroxy-L-phenylalanine (L-DOPA)
dopamine
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)

Catecholamines

Application #:
TI0668
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
beta-endosulfan
alpha-endosulfan
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Presto FF-C18 (150 x 4.6mm)

Endosulfan isomers

Application #:
TI0667
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
beta-endosulfan
alpha-endosulfan
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Endosulfan isomers

Application #:
TI0665
Type:
Application Note
Categories:
Industrial Chemical
Compounds:
Cr(III) and Cr(VI)
Column(s):
Scherzo SM-C18 (50 x 3.0mm)

LC-ICP-MS analysis of Cr(III) and Cr(VI)

Application #:
TI0664
Type:
Application Note
Categories:
Industrial Chemical
Environmental and Agricultural
Compounds:
glyphosate
AMPA (metabolite)
glufosinate
MPPA (metabolite)
bialaphos
Column(s):
Scherzo SM-C18 (30 x 3.0mm)

LC-MS analysis of phosphorus-containing amino acid type herbicides and their metabolites

Application #:
TI0663
Type:
Application Note
Categories:
Polysaccharides
Compounds:
dextran sulfate sodium salt
Column(s):
Presto FF-C18 (150 x 4.6mm)

Dextran Sulfate

Application #:
TI0662
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Compounds:
penicillin G
methicillin
ampicillin
amoxicillin
carbenicillin
Column(s):
Scherzo SM-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

Penicillin Antibiotics

Application #:
TI0660
Type:
Application Note
Categories:
Amino acids and peptides
Compounds:
Collagenpeptide
enzyme hydrolyzed; collagenpeptide acid hydrolyzed
Column(s):
Presto FF-C18 (250 x 4.6mm)

Collagenpeptide

Application #:
TI0659
Type:
Application Note
Categories:
Food Chemistry
Compounds:
(E)-beta-farnesene
Column(s):
Scherzo SM-C18 (250 x 4.6mm)
Cadenza CD-C18 (250 x 4.6mm)
Cadenza CL-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Farnesene isomers

Application #:
TI0658
Type:
Application Note
Categories:
LC-MS
Pharmaceuticals and Cosmetics
Compounds:
chloramphenicol (CP)
Column(s):
Cadenza CD-C18 (150 x 2.0mm)

LC-MS/MS analysis of chloramphenicol (CP)

Application #:
TI0657
Type:
Application Note
Categories:
LC-MS
Industrial Chemical
Compounds:
malachite green (MG)
leucomalachite green (LMG)
Column(s):
Cadenza CD-C18 (150 x 2.0mm)

LC-MS/MS analysis of [leuco]malachite green (MG, LMG)

Application #:
TI0656
Type:
Application Note
Categories:
LC-MS
Pharmaceuticals and Cosmetics
Compounds:
clenbuterol
Column(s):
Cadenza CD-C18 (150 x 2.0mm)

LC-MS/MS analysis of clenbuterol (CBR)

Application #:
TI0655
Type:
Application Note
Categories:
LC-MS
Pharmaceuticals and Cosmetics
Compounds:
3-amino-5-morpholinomethyl-2oxazolidinone
3-amino-2-oxazolidinone
1-aminohydantionq
Column(s):
Cadenza CD-C18 (150 x 2.0mm)

LC/MS analysis of nitrofurans metabolites (AOZ, AMOZ, AHD)

Application #:
TI0654
Type:
Application Note
Categories:
LC-MS
Industrial Chemical
Compounds:
ammonium acetate
acetonitrile
Column(s):
Unison UK-C18 (250 x 2.0mm)

LC/MS analysis of perfluorinated organic compounds (PFC's) in seawater

Application #:
TI0653
Type:
Application Note
Categories:
Amino acids and peptides
Compounds:
water
acetonitrile
formic acid
Column(s):
Presto FF-C18 (250 x 4.6mm)

The effect of TFA modifier on peptide mapping

Application #:
TI0652
Type:
Application Note
Categories:
Column Performance
Biochemistry/Metabolism
LC-MS
Compounds:
alanine
N
N-dimethylglycine
serine
fumaric acid
succinic acid
cysteine
oxaloacetic acid
Gly-Gly
malic acid
alpha-ketoglutarate
citric acid
2-methylhippuric acid
gamma-D-glutamylglycine
salbutomal
AMP
ribo-avin
Phe-gly-phe-gly
s-(p-nitrobenzyl) glutathione
s-(p-azidophenacyl)glutathione
taurocholic acid
O-beta-D-glucuronosyl-naphthol AS-BI
Leucine enkephalin
Arg-Pro-Pro-Gly-Phe
Reserpine
GSSG
Arg-Lys-Asp-Val-Tyr
Coenzyme A
Acetyl CoA
Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr (peptide T)
bradykinin fragment 1-8
Tyr-tyr-tyr-tyr-tyr-tyr
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

Exploring different C18 stationary phases for metabolites profiling

Application #:
TI0651
Type:
Application Note
Categories:
Polysaccharides
Compounds:
sodium chondroitin sulfate
hyaluronic acid sodium salt
Column(s):
Presto FF-C18 (150 x 4.6mm)

Hyaluronic acid, chondrotin sulfuric acid

Application #:
TI0650
Type:
Application Note
Categories:
Industrial Chemical
Compounds:
Triton X-100
Igepal CO-720
Column(s):
Cadenza CW-C18 (50 x 3.0mm)

Surfactants

Application #:
TI0649
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Quinic acid
Malic acid
Tartaric acid
Succinic acid
citric acid
gallic acid
lactic acid
protocatechuic acid
4HPAC
chlorogenic acid
syringic acid
gentisic acid
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

Simulatneous HPLC/LT-ELSD Analysis of Organic and Phenolic Acids

Application #:
TI0648
Type:
Application Note
Categories:
Biochemistry/Metabolism
Vitamins
Compounds:
B1:thiamine
B8:myo-Inositol
B6:Pyridoxine
C:Ascorbic acid
B5:Panthothenic acid
B9: Folic acid
B12: Cyanocobalamin
B2:Riboflavin
B7: Biotin
A:Retinol
K2:Menaquinone
D2:Ergocalciferol
D3:Cholecalciferol
E:a-Tocopherol
K1: Phylloquinone
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

Simulatneous HPLC/LT-ELSD Analysis of Water Soluble and Fat Soluble Vitamins

Application #:
TI0647
Type:
Application Note
Categories:
Polysaccharides
Saccharides
Food Chemistry
Compounds:
Glycerol
rhamnose
erythritol
arabinose
xylose
fructose
sorbitol
mannose
galactose
glucose
inositol
sucrose
maltulose
lactose
maltose
trehalose
raffinose
maltotriose
nystose
maltotetraose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Simultaneous HPLC/LT-ELSD Analysis of Polyols, Mono-,Di- and Oligosaccarides

Application #:
TI0646
Type:
Application Note
Categories:
Amino acids and peptides
Compounds:
water
acetonitrile
formic acid
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

Peptide Mapping (50 C formic acid)

Application #:
TI0645
Type:
Application Note
Categories:
LC-MS
Botanicals
Compounds:
Amanitin
Phalloidin
Cochicine
Scopolamine
Mesaconitine
Aconitine
Jesaconitine
Solanine
Chaconine
Hypaconitine
Atropine
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

LC-MS/MS Application: Simultaneous determination of phytotoxins in urine

Application #:
TI0644
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
albumin (eggs)
beta-glucosidase (almond)
thyroglobulin (porcin thyroid)
lectin (concanavalin A from Jack bean)
alpha-chymotryosinogen A (bovine pancreas)
monoclonal antibody (anti-AFP)
myoglobin (equine skeletal muscle)
alpha-lactalbumin (bovine milk)
papin (papaya latex)
albumin (human serum)
lactoferrin (bovine milk)
lysozyme (chicken egge white)
cytochrome c (horse heart)
Column(s):
Presto FF-C18 (75 x 4.6mm)

Protein separation on non-porous ODS column

Application #:
TI0643
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
albumin (eggs)
beta-glucosidase (almond)
thyroglobulin (porcin thyroid)
lectin (concanavalin A
from Jack bean)
alpha-chymotryosinogen A (bovine pancreas)
myoglobin (equine skeletal muscle)
alpha-lactalbumin (bovine milk)
apain (papya latex)
albumin (human serum)
lactoferrin (bovine milk)
lysozyme (chicken egg white)
cytochrome c (horse heart)
myosin (rabbit muscle)
alpha0casein (bovine milk)
alpha-amylase (Bacillus)
xanthine oxidase (buttermilk)
Column(s):
Presto FF-C18 (75 x 4.6mm)

Protein separation on non-porous ODS column

Application #:
TI0642
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
vanillylmandelic acid
mandelic acid
homovanillic acid
dopamine
Column(s):
Scherzo SM-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Scherzo is able to separate mandelic acid and dopamine, which coelute on a conventional C18 column (Unison UK-C18). This is possible because the ionic ligands recognize the small structural differences between the two compounds.

Application #:
TI0641
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
vanillylmandelic acid
mandelic acid
homovanillic acid
dopamine
Column(s):
Scherzo SM-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Catecholamine metabolites

Application #:
TI0640
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Compounds:
dl-chlorpheniramine
dextromethorphan
propylparaben
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Cold medicine compounds

Application #:
TI0639
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Compounds:
dl-chlorpheniramine
dextromethorphan
propylparaben
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CW-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)
Unison UK-C8 (150 x 4.6mm)
Unison UK-Phenyl (150 x 4.6mm)

Cold medicine compounds

Application #:
TI0638
Type:
Application Note
Categories:
Food Chemistry
Compounds:
theaflavin
theaflavin-3-O-gallate
theaflavin-3'-O-gallate
theaflavin-3
.'-di-O-gallate
Column(s):
Cadenza CL-C18 (100 x 4.6mm)

Theaflavins

Application #:
TI0636
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
alpha-lactalbumin (bovine milk)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for alpha-lactalbumin separation

Application #:
TI0635
Type:
Application Note
Categories:
Column Performance
Proteins
Botanicals
Compounds:
cytochrome c (horse heart)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for cytochrome c separation

Application #:
TI0634
Type:
Application Note
Categories:
Column Performance
Proteins
Biochemistry/Metabolism
Compounds:
thyroglobulin( porcin thyroid)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for thyroglobulin separation

Application #:
TI0633
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
lectin (concanavalin A)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for lectin separation

Application #:
TI0632
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
albumin(chicken egg)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for ovalbumin separation

Application #:
TI0631
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
albumin(bovine serum)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for BSA separation

Application #:
TI0630
Type:
Application Note
Categories:
Column Performance
Proteins
Biochemistry/Metabolism
Compounds:
insulin
Column(s):
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope for insulin separation

Application #:
TI0629
Type:
Application Note
Categories:
Column Performance
Biochemistry/Metabolism
Amino acids and peptides
Compounds:
angiotensin II
Column(s):
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope for angiotensin II serparation

Application #:
TI0628
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
esculin
esculetin
hydroxycoumarin
scopoletin
coumarin
dihydrocoumarin
warfarin
Column(s):
Scherzo SM-C18 (150 x 4.6mm)

Coumarins

Application #:
TI0627
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
abumin (human serum)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for HSA serpation

Application #:
TI0626
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
abumin (human serum)
Column(s):
Presto FF-C18 (250 x 4.6mm)

Gradient mixer volume affecting HSA separation

Application #:
TI0625
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
plyethylene glycol
Column(s):
Cadenza CW-C18 (250 x 4.6mm)

Polyethylene glycol (PEG)

Application #:
TI0624
Type:
Application Note
Categories:
Proteins
Compounds:
IgG from human serum
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

IgG from human serum

Application #:
TI0623
Type:
Application Note
Categories:
Proteins
Compounds:
monoclonal antibody
polyclonal antibody
Column(s):
Presto FF-C18 (250 x 3.0mm)

Monoclonal and polyclonal antibodies

Application #:
TI0622
Type:
Application Note
Categories:
Proteins
Compounds:
mAb
Column(s):
Presto FF-C18 (250 x 4.6mm)

Gradient Slopes affecting mAb separation

Application #:
TI0621
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
plerixafor
Column(s):
Unison UK-C18 (150 x 4.6mm)

Plerixafor (Chemokine receptor antagonist)

Application #:
TI0620
Type:
Application Note
Categories:
Proteins
Compounds:
Polyclonal IgG
Column(s):
Presto FF-C18 (250 x 4.6mm)

Polyclonal IgG (from Human Serum)

Application #:
TI0619
Type:
Application Note
Categories:
Proteins
Compounds:
Hemocyanin and hemoglobin
Column(s):
Presto FF-C18 (250 x 4.6mm)

Hemocyanin and hemoglobin

Application #:
TI0618
Type:
Application Note
Categories:
Polysaccharides
Compounds:
Starch
amylopectin
amylose
Column(s):
Presto FF-C18 (150 x 4.6mm)

Starch, amylopectin, amylose (from potato)

Application #:
TI0616
Type:
Application Note
Categories:
Polysaccharides
Compounds:
hyaluronic acid
Column(s):
Presto FF-C18 (150 x 4.6mm)

Hyaluronic acid

Application #:
TI0612
Type:
Application Note
Categories:
Oligosaccharides
Biochemistry / Metabolism
Botanicals
Compounds:
Unsaturated Chondro-Disaccharides
Column(s):
Unison UK-Amino (250 x 3.0mm)

Unsaturated Chondro-Disaccharides

Application #:
TI0611
Type:
Application Note
Categories:
Food Chemistry
Compounds:
maltol
ethyl maltol
Column(s):
Cadenza CW-C18 (150 x 3.0mm)
Unison UK-Phenyl (150 x 3.0mm)
Presto FF-C18 (150 x 4.6mm)

Maltol, Ethyl Maltol

Application #:
TI0610
Type:
Application Note
Categories:
Industrial Chemical
Pharmaceuticals & Cosmetics
Compounds:
zinc pyrithione
copper pyrithione
Column(s):
Unison UK-Phenyl (50 x 3.0mm)

Zinc Pyrithione, Copper Pyrithione

Application #:
TI0609
Type:
Application Note
Categories:
Industrial Chemical
Pharmaceuticals & Cosmetics
Compounds:
chromium
copper
zinc
magnesium
calcium
potassium
sodium
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Cations

Application #:
TI0608
Type:
Application Note
Categories:
Saccharides
LC-MS
Biochemistry / Metabolism
Compounds:
fucose
xylose
N-acetylgalactosamine
N-acetylglucosamine
mannose
galactose
glucose
N-acetylneuraminic acid
N-glycolylneuraminic acid
Column(s):
Unison UK-Amino (250 x 2.0mm)

LC-MS(MRM) Application for Monosaccharides

Application #:
TI0607
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
sodium gluconate
acetic acid
lactic acid
glyoxylic acid
formic acid
Column(s):
Unison UK-Amino (250 x 4.6mm)

Organic acids (Anion exchange mode)

Application #:
TI0606
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
cellulose acetate (acetyl content 40 wt. %
ca.30 kDa)
Column(s):
Presto FF-C18 (150 x 4.6mm)

Cellulose acetate

Application #:
TI0605
Type:
Application Note
Categories:
Amino Acids and Peptides
Compounds:
Tryptic digest of alpha-casein
Column(s):
Cadenza CW-C18 (250 x 3.0mm)
Presto FF-C18 (250 x 3.0mm)

Peptide mapping

Application #:
TI0604
Type:
Application Note
Categories:
Synthetic Polymers
Pharmaceuticals & Cosmetics
Compounds:
poly (DL-lactide)
poly (DL-lactide-co-glycolide)
Column(s):
Presto FF-C18 (30 x 4.6mm)

Biodegradable polymer ( poly (DL-lactide) and poly (DL-lactide-co-glycolide) )

Application #:
TI0603
Type:
Application Note
Categories:
Synthetic Polymers
Pharmaceuticals & Cosmetics
Compounds:
poly (DL-lactide)
poly (DL-lactide-co-glycolide)
Column(s):
Unison UK-Amino (150 x 3.0mm)

Biodegradable polymer ( poly (DL-lactide) and poly (DL-lactide-co-glycolide) )

Application #:
TI0602
Type:
Application Note
Categories:
Synthetic Polymers
Pharmaceuticals & Cosmetics
Compounds:
poly (DL-lactide)
poly (DL-lactide-co-glycolide)
Column(s):
Cadenza CW-C18 (150 x 3.0mm)

Biodegradable polymer ( poly (DL-lactide) and poly (DL-lactide-co-glycolide) )

Application #:
TI0601
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
N-methylthiourea
urea
3-hydroxyphenylurea
hydroxyurea
Column(s):
Unison UK-Amino (250 x 4.6mm)

Urea and related compounds

Application #:
TI0600
Type:
Application Note
Categories:
Vitamins
LC-MS
Compounds:
thiamine
pyridoxine
nicotinic acid
nicotinamide
pantothenic acid
cyanocobalamin
riboflavin
botin
folic acid
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

Analysis of Water Soluble Vitamins using LCMS-2020

Application #:
TI0599
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
NAD
NADP
NADH
NADPH
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Coenzymes (Nicotinamide adenine dinucleotides)

Application #:
TI0598
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
NAD
NADP
NADH
NADPH
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Coenzymes (Nicotinamide adenine dinucleotides)

Application #:
TI0597
Type:
Application Note
Categories:
Amino acids and peptides
Compounds:
Tau
Gly
Ser
Ala
Lys
Asp
Glu
Arg
Pro
Car. Val
Met. IIe
Leu
Phe
Trp
Column(s):
Cadenza CD-C18 (250 x 4.6mm)

RP analysis of intact amino acids using Corona CAD

Application #:
TI0596
Type:
Application Note
Categories:
Saccharides
Compounds:
Xylose
Fructose
Mannose
Glucose
Sucrose
Lactose
Maltose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Improving of analysis time and peak recovery for saccharide separation

Application #:
TI0595
Type:
Application Note
Categories:
Saccharides
Compounds:
Fructose
Sorbitol
Glucose
Sucrose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Saccharide analysis for beverages using Corona CAD

Application #:
TI0594
Type:
Application Note
Categories:
Saccharides
Compounds:
Xylose
Fructose
Mannose
Glucose
Sucrose
Lactose
Maltose
Column(s):
Unison UK-Amino (250 x 3.0mm)

High sensitivity analysis for saccharides using Corona CAD

Application #:
TI0593
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
plerixafor
Column(s):
Unison UK-C18 (150 x 4.6mm)

Plerixafor (Chemokine receptor antagonist)

Application #:
TI0592
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
cetirizine
Column(s):
Scherzo SM-C18 (150 x 4.6mm)
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CW-C18 (150 x 4.6mm)
Cadenza CL-C18 (150 x 4.6mm)
Cadenza HS-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)
Unison UK-C8 (150 x 4.6mm)
Unison UK-Phenyl (150 x 4.6mm)

Cetirizine (Antihistamine)

Application #:
TI0591
Type:
Application Note
Categories:
Proteins
Compounds:
PEGylated protein
60 kDa
(20 kDa protein + 40 kDa PEG)
Column(s):
Cadenza CW-C18 (250 x 3.0mm)

PEGylated protein (2)

Application #:
TI0590
Type:
Application Note
Categories:
Proteins
Compounds:
PEGylated protein
60 kDa (20 kDa protein + 40 kDa PEG)
Column(s):
Presto FF-C18 (150 x 4.6mm)

PEGylated protein (2)

Application #:
TI0589
Type:
Application Note
Categories:
Proteins
Compounds:
PEGylated protein
Column(s):
Presto FF-C18 (30 x 4.6mm)
Presto FF-C18 (150 x 4.6mm)

PEGylated protein

Application #:
TI0588
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
adenosine 5'- monophosphate disodium (AMP) guanosine 5'- monophosphate disodium (GMP)
adenosine 3'
5'-cyclic monophosphate sodium (cAMP)
guanosine 3'
5'-cyclic monophosphate sodium (cGMP)
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Cyclic mononucleotides (cAMP, cGMP)

Application #:
TI0587
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
ribonucleic acid (from yeast)
Column(s):
Presto FF-C18 (30 x 4.6mm)
Presto FF-C18 (150 x 4.6mm)

Ribonucleic acid (RNA)

Application #:
TI0586
Type:
Application Note
Categories:
Polysaccharides
Compounds:
Proteoglycan (from nasal cartilage of salmon head
ca. 450 kDa)
Column(s):
Presto FF-C18 (150 x 4.6mm)

Proteoglycan

Application #:
TI0585
Type:
Application Note
Categories:
Polysaccharides
Compounds:
amylose
Column(s):
Presto FF-C18 (150 x 4.6mm)

Amylose

Application #:
TI0584
Type:
Application Note
Categories:
Polysaccharides
Compounds:
fucoidan (from Fucus vesiculosus)
Column(s):
Presto FF-C18 (30 x 4.6mm)
Presto FF-C18 (150 x 4.6mm)

Fucoidan

Application #:
TI0583
Type:
Application Note
Categories:
Polysaccharides
Compounds:
mannan
Column(s):
Presto FF-C18 (30 x 4.6mm)
Presto FF-C18 (150 x 4.6mm)

Mannan

Application #:
TI0582
Type:
Application Note
Categories:
Proteins
Compounds:
concanavalin A (from Jack Bean
Column(s):
Presto FF-C18 (150 x 4.6mm)

Lectin (concanavalin A)

Application #:
TI0581
Type:
Application Note
Categories:
Industrial Chemical
Environmental and Agricultural
Compounds:
Bisphenol A
Column(s):
Scherzo SM-C18 (75 x 4.6mm)
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CW-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Cadenza HS-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Bisphenol A

Application #:
TI0580
Type:
Application Note
Categories:
Proteins
Compounds:
poly-L-lysine
poly-DL-alanine
Column(s):
Presto FF-C18 (250 x 4.6mm)

Polyamino acids

Application #:
TI0579
Type:
Application Note
Categories:
Polysaccharides
Compounds:
dextran
Column(s):
Presto FF-C18 (250 x 4.6mm)

Dextrans

Application #:
TI0578
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
Igepal CO-720
Triton X-100
Column(s):
Presto FF-C18 (30 x 4.6mm)

Surfactants

Application #:
TI0577
Type:
Application Note
Categories:
Polysaccharides
Compounds:
chondroitin sulfuric acid
dermatan sulfuric acid
heparin
Column(s):
Presto FF-C18 (250 x 4.6mm)

Mucopolysaccharides

Application #:
TI0576
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
salmon DNA / DNase
Column(s):
Presto FF-C18 (250 x 4.6mm)

Double-stranded DNA fragments

Application #:
TI0575
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
poly DL-lactide
Column(s):
Presto FF-C18 (150 x 4.6mm)

Poly DL-Lactide

Application #:
TI0574
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
polystyrene
Column(s):
Presto FF-C18 (150 x 4.6mm)

Polystyrene

Application #:
TI0573
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
polyvinyl acetate
Column(s):
Presto FF-C18 (150 x 4.6mm)

Polyvinyl acetate

Application #:
TI0572
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
polyvinyl alcohol
Column(s):
Presto FF-C18 (150 x 4.6mm)

Polyvinyl alcohol (PVA)

Application #:
TI0571
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
1 polyethylene glycol
Column(s):
Presto FF-C18 (150 x 4.6mm)

Polyethylene glycols (PEG)

Application #:
TI0570
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
polyvinylpyrrolidone
Column(s):
Presto FF-C18 (150 x 4.6mm)

Polyvinylpyrrolidone (PVP)

Application #:
TI0569
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
hydroxypropylcellulose
Column(s):
Presto FF-C18 (150 x 4.6mm)

Hydroxypropylcellulose

Application #:
TI0568
Type:
Application Note
Categories:
Synthetic Polymers
Compounds:
carboxymethylcellulose sodium salt
Column(s):
Presto FF-C18 (150 x 4.6mm)

Carboxymethylcellulose

Application #:
TI0567
Type:
Application Note
Categories:
Proteins
Compounds:
gelatin (from bovine bone)
Column(s):
Presto FF-C18 (150 x 4.6mm)

Gelatin

Application #:
TI0566
Type:
Application Note
Categories:
Polysaccharides
Compounds:
pullulan
Column(s):
Presto FF-C18 (150 x 4.6mm)

Pullulan

Application #:
TI0565
Type:
Application Note
Categories:
Polysaccharides
Compounds:
hyaluronic acid sodium salt (from rooster comb)
Column(s):
Presto FF-C18 (150 x 4.6mm)

Hyaluronic acid

Application #:
TI0564
Type:
Application Note
Categories:
Polysaccharides
Compounds:
L-guluronic acid
D-mannuronic acid
alginic acid sodium salt
Column(s):
Presto FF-C18 (150 x 4.6mm)

Alginic acid

Application #:
TI0563
Type:
Application Note
Categories:
Polysaccharides
Compounds:
chondroitin sulfuric acid
kappa-carrageenan
iota-carrageenan
Column(s):
Presto FF-C18 (250 x 4.6mm)

Chondroitin sulfuric acid, carrageenan

Application #:
TI0562
Type:
Application Note
Categories:
Polysaccharides
Compounds:
lipopolysaccharide
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (150 x 4.6mm)

Lipopolysaccharide (from E.coli O127)

Application #:
TI0561
Type:
Application Note
Categories:
Polysaccharides
Compounds:
pectin
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (150 x 4.6mm)

Pectin

Application #:
TI0560
Type:
Application Note
Categories:
Proteins
Compounds:
poly-gamma-glutamic acid (sodium salt)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (150 x 4.6mm)

Polyglutamic acid

Application #:
TI0559
Type:
Application Note
Categories:
Vitamins
Compounds:
alpha-tocopherol
beta-tocopherol
gamma-tocotrienol
alpha-tocotrienol
gamma-tocopherol
delta-tocopherol
beta-tocotrienol
delta-tocotrienol
Column(s):
Unison UK-Silica (150 x 4.6mm)
Unison UK-Amino (150 x 4.6mm)

Tocopherols and tocotrienols

Application #:
TI0558
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
pUC18 (2
686 bp) pBR322 (4
361 bp)
Column(s):
Presto FF-C18 (250 x 4.6mm)

Plasmid DNA

Application #:
TI0557
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
DNA
Column(s):
Presto FF-C18 (150 x 4.6mm)

DNA from salmon spermary

Application #:
TI0556
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
glyphosate
surfactant
Column(s):
Scherzo SM-C18 (250 x 3.0mm)

Herbicide formulation (glyphosate)

Application #:
TI0555
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
glufosinate
fosetyl
glyphosate
paraquat dichloride
diquat dibromide
Column(s):
Scherzo SM-C18 (250 x 3.0mm)

Organophosphorous pesticides, quaternary ammonium herbicides

Application #:
TI0554
Type:
Application Note
Categories:
Vitamins
Compounds:
alpha-carotene and beta-carotene
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CL-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Alpha-carotene and beta-carotene

Application #:
TI0553
Type:
Application Note
Categories:
Industrial Chemical
Compounds:
diisononyl phthalate (DINP)
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

Diisononyl phthalate

Application #:
TI0552
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
sodium arsenite
dimethylarsinic acid (DMAA)
methylarsonic acid (MAA)
disodium hydrogenarsenate heptahydrate
Column(s):
Scherzo SM-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

Arsenic compounds

Application #:
TI0551
Type:
Application Note
Categories:
Industrial Chemical
Compounds:
Chromium (III) nitrate and ammonium chromate(VI)
Column(s):
Scherzo SM-C18 (50 x 3.0mm)

Chromium (III) nitrate and ammonium chromate(VI)

Application #:
TI0550
Type:
Application Note
Categories:
Food Chemistry
Compounds:
sucralose
Column(s):
Unison UK-C18 (250 x 4.6mm)

Sucralose by reversed-phase analysis

Application #:
TI0549
Type:
Application Note
Categories:
Industrial Chemical
Environmental and Agricultural
Compounds:
sodium thiosulfate
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Sodium thiosulfate

Application #:
TI0548
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
succinic acid
malic acid
fumaric acid
malonic acid
citric acid
tartaric acid
maleic acid
Column(s):
Scherzo SM-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

Organic acids (2)

Application #:
TI0547
Type:
Application Note
Categories:
Industrial Chemical
Compounds:
butylbenzyl phthalate (BBP)
di-2-ethylhexyl phthalate (DEHP)
diisononyl phthalate (DINP)
dibutyl phthalate (DBP)
di-n-octyl phthalate (DNOP)
diisodecyl phthalate (DIDP)
Column(s):
Cadenza CW-C18 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Phthalate esters

Application #:
TI0546
Type:
Application Note
Categories:
Industrial Chemical
Compounds:
p-hydroxybenzoic acid
m-hydroxybenzoic acid
terephthalic acid
isophthalic acid
homophthalic acid
o-phthalic acid
hydroxybenzoic acid
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Aromatic carboxylic acids

Application #:
TI0545
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Probucol
Column(s):
Cadenza HS-C18 (150 x 3.0mm)

Anti-hyperlipidemic drug in bovine serum (probucol)

Application #:
TI0544
Type:
Application Note
Categories:
Industrial Chemical
Pharmaceuticals & Cosmetics
Compounds:
Benzathonium chloride
Column(s):
Scherzo SM-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

Benzathonium chloride

Application #:
TI0543
Type:
Application Note
Categories:
Industrial Chemical
Pharmaceuticals & Cosmetics
Compounds:
benzalkonium chloride
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Benzalkonium chloride

Application #:
TI0542
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
succinic acid
lactic acid
sodium gluconate
malic acid
glyoxylic acid
fumaric acid
maloinic acid
citric acid
tartaric acid
maleic acid
Column(s):
Unison UK-Amino (250 x 3.0mm)

Organic Acids

Application #:
TI0541
Type:
Application Note
Categories:
Industrial Chemical
Food chemistry
Compounds:
Sodium sulfite
Column(s):
Scherzo SM-C18 (250 x 3.0mm)

Sodium Sulfite in Red Wine

Application #:
TI0540
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
glyphosate
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Glyphosate

Application #:
TI0539
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
adenine
guanine
hypoxanthine
xanthine
Column(s):
Scherzo SM-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Purine bases

Application #:
TI0538
Type:
Application Note
Categories:
Environmental and Agricultural
Industrial Chemical
Compounds:
naphthalene
fluorene
acenaphthene
anthracene
benzo(j)fluoranthene
benzo(e)pyrene
Column(s):
Cadenza CL-C18 (250 x 3.0mm)

Polycyclic aromatic hydrocarbons

Application #:
TI0537
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
streptomycin sulfate
amikacin disulfate
kanamycin sulfate
tobramycin sulfate
gentamicin sulfate
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Aminoglycoside antibiotics

Application #:
TI0536
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Biochemistry / Metabolism
Compounds:
N-methylhydantoin
methimazole
N-methylthiourea
2-mercaptoimidazole
Column(s):
Unison UK-Amino (250 x 3.0mm)

Methimazole and potential metabolites

Application #:
TI0535
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Botanicals
Saccharides
Compounds:
nojirimycin bisulfite
1-deoxynojirimycin hydrochloride
1-deoxymannojirimycin hydrochloride
Column(s):
Scherzo SM-C18 (250 x 4.6mm)

Nojirimycin relative compounds

Application #:
TI0534
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
hydroxyzine
pamoic acid
Column(s):
Scherzo SM-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)

Hydroxyzine pamoate

Application #:
TI0533
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
fumaric acid
clemastine
Column(s):
Scherzo SM-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)

Clemastine fumarate

Application #:
TI0532
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
tipepidine
hibenzic acid
Column(s):
Scherzo SM-C18 (50 x 3.0mm)

Tipepidine hibenzate

Application #:
TI0531
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
maleic acid
dl-chlorpheniramine
Column(s):
Scherzo SM-C18 (50 x 3.0mm)

Chlorpheniramine maleate

Application #:
TI0530
Type:
Application Note
Categories:
Biochemistry / Metabolism
Industrial Chemical
Compounds:
methyl lactate
lactic acid
levulinic acid
methyl levulinate
Column(s):
Scherzo SM-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Levulinic Acid, Lactic Acid and methyl Esters

Application #:
TI0529
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
ADP
AMP
ATP
Column(s):
Scherzo SM-C18 (30 x 3.0mm)
Unison UK-C18 (30 x 3.0mm)

Adenosine Phosphates

Application #:
TI0528
Type:
Application Note
Categories:
Amino acids and peptides
Compounds:
Phe
Leu
Ile
Trp
Met
Val
Pro
Tyr
Thr
Ala
Arg
Ser
Lys
His
Gly
Asn
gln
Cys
Glu
Asp
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Amino acids: normal phase

Application #:
TI0527
Type:
Application Note
Categories:
Amino acids and peptides
Compounds:
acidic amino acids
hydrophilic amino acids
aliphatic amino acids
branched-chain amino acids
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Amino acids: acidic condition

Application #:
TI0526
Type:
Application Note
Categories:
Amino acids and peptides
Compounds:
basic amino acids
hydrophilic amino acids
aliphatic amino acids
branched-chain amino acids
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Amino acids: neutral pH condition

Application #:
TI0525
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
doxepine
desipramine
imipramine
nortriptyline
amitriptyline
trimipramine
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Basic compounds: Antidepressant drugs

Application #:
TI0524
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
L-carnosine
L-anserine nitrate
Inosine 5' monophosphate dipotassium
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Anserine and Related Compounds

Application #:
TI0523
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
acetic acid
trifluoroacetic acid
trichloroacetic acid
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Acetic Acid and Trihaloacetic Acids

Application #:
TI0522
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
CMP
UMP
dCMP
dUMP
IMP
GMP
dTMP
AMP
dGMP
dAMP
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Mononucleotides

Application #:
TI0521
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
glutathione reduced (GSH)
glutathione oxidized (GSSG)
Column(s):
Scherzo SM-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)

Glutathiones

Application #:
TI0520
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Biochemistry / Metabolism
Compounds:
sodium chloride
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Sodium Chloride

Application #:
TI0519
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
DL-mevalonolactone
DL-mevalonic acid
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Mevalonolactone and Mevalonic Acid

Application #:
TI0518
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
sodium gluconate
glyoxylic acid
lactic acid
malic acid
succinic acid
malonic acid
citric acid
fumaric acid
maleic acid
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Organic Acids

Application #:
TI0517
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
beta-estradiol 17-(beta-glucuronide)
17beta-estradiol
beta-estradiol 3
17 disulfate
Column(s):
Scherzo SM-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)

Estradiol and Metabolites

Application #:
TI0516
Type:
Application Note
Categories:
Saccharides
LC-MS
Compounds:
fructose
sucrose
Column(s):
Unison UK-Amino (150 x 2.0mm)

LC-MS/MS Application for Saccharides

Application #:
TI0515
Type:
Application Note
Categories:
Saccharides
LC-MS
Compounds:
fructose
glucose
Column(s):
Unison UK-Amino (150 x 2.0mm)

LC-MS/MS Application for Saccharides

Application #:
TI0514
Type:
Application Note
Categories:
Botanicals
Compounds:
gibberellin A3
3-indoleacetic acid
abscisic acid
3-indolepropionic acid
jasmonic acid
3-indolebutyric acid
gibberellin A4
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Cadenza HS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)
Unison UK-Phenyl (150 x 3.0mm)

Phytohormones

Application #:
TI0513
Type:
Application Note
Categories:
Botanicals
Compounds:
gibberellin A3
3-indoleacetic acid
abscisic acid
3-indolepropionic acid
jasmonic acid
3-indolebutyric acid
gibberellin A4
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Cadenza HS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)
Unison UK-Phenyl (150 x 3.0mm)

Phytohormones

Application #:
TI0512
Type:
Application Note
Categories:
Biochemistry/Metabolism
LC-MS
Compounds:
Sphingomyelin from sheep erythrocytes
Column(s):
Cadenza CD-C18 (150 x 2.0mm)

Identification of sphingomyelin molecular species with LC-MS/MS

Application #:
TI0511
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Zanamivir (Anti-flu medicine)
Column(s):
Unison UK-C18 (150 x 4.6mm)
Unison UK-Silica (150 x 4.6mm)
Unison UK-Amino (150 x 4.6mm)

Zanamivir (Anti-flu medicine)

Application #:
TI0510
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Oseltamivir phosphate (Anti-flu medicine)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Oseltamivir phosphate (Anti-flu medicine)

Application #:
TI0509
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Paroxetine HCl (Antidepressant)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Paroxetine HCl (Antidepressant)

Application #:
TI0508
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Rilmazafone HCl (Benzodiazepine hypnotic)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Rilmazafone HCl (Benzodiazepine hypnotic)

Application #:
TI0507
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Zopiclone (Nonbenzodiazepine hypnotic)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Zopiclone (Nonbenzodiazepine hypnotic)

Application #:
TI0506
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Codeine phosphate (Narcotic antitussive)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Codeine phosphate (Narcotic antitussive)

Application #:
TI0502
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Column Performance
Compounds:
phenyltrimethylammnonium
benzenesulfonic acid
benzamide
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

The Scherzo SM-C18 column shows excellent batch to batch reproducibility for anionic, cationic, and neutral compounds.

Application #:
TI0500
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Steroids
Column Performance
Compounds:
acetaminophen
1-hydroxyN 7-azabenzotriazole
prednisolone
methyl 3-aminoS 2-thiophenecarboxylate
6-alphamethylprednisolone
corticosterone
4-aminobenzophenone
propylparaben
Column(s):
Scherzo SM-C18 (75 x 4.6mm)

The Scherzo SM-C18 column shows excellent batch to batch reproducibility for these small polar molecules.

Application #:
TI0499
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Pranoprofen (Nonsteroid anti-inflammatory drug:NSAID)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Pranoprofen (Nonsteroid anti-inflammatory drug:NSAID)

Application #:
TI0498
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Naftopidil (alpha1-receptor blocker)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Naftopidil (alpha1-receptor blocker)

Application #:
TI0497
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Furosemide (Diuretic)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Furosemide (Diuretic)

Application #:
TI0496
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Menatetrenone (Vitamin K)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Menatetrenone (Vitamin K)

Application #:
TI0495
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Mequitazine
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Mequitazine (Antihistamine)

Application #:
TI0494
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics / Steroids
Compounds:
Betamethasone
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Betamethasone (Antihistamine:Adrenal corticosteroid)

Application #:
TI0493
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
urine
Column(s):
Cadenza HS-C18 (100 x 2.0mm)

LC-Q-TOF-MS application for high-throughput non-targeted metabolomics with a hybrid ODS column

Application #:
TI0492
Type:
Application Note
Categories:
Vitamins
Compounds:
pyridoxine
folic acid
calcium pantothenate
riboflavin
Column(s):
Cadenza CW-C18 (150 x 3.0mm)

Water-soluble vitamins

Application #:
TI0491
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
Sphinganine-1-Phosphate (Dihydrosphingosine-1-phosphate)
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Sphinganine-1-PO4)

Application #:
TI0490
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
Sphingosine-1-Phosphate
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Sphingosine-1-PO4)

Application #:
TI0489
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
Sphingosine
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Sphingosine)

Application #:
TI0488
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
Sphinganine (Dihydrosphingosine)
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Sphinganine)

Application #:
TI0487
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
C16 Ceramide
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Ceramide)

Application #:
TI0486
Type:
Application Note
Categories:
Botanicals
Compounds:
hesperidin
naringin
neohesperidin
neohesperidin dihydrochalcone
Column(s):
Unison UK-Amino (150 x 3.0mm)

Flavanone glycosides

Application #:
TI0485
Type:
Application Note
Categories:
Botanicals
Compounds:
naringin
hesperidin
neohesperidin
neohesperidin dihydrochalcone
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)

Flavanone glycosides

Application #:
TI0484
Type:
Application Note
Categories:
Food Chemistry / Industrial Chemical
Compounds:
Denatonium benzoate
Column(s):
Cadenza CD-C18 (50 x 3.0mm)

Denatonium benzoate

Application #:
TI0483
Type:
Application Note
Categories:
Food Chemistry / Industrial Chemical
Compounds:
denatonium benzoate
Column(s):
Cadenza CD-C18 (50 x 3.0mm)
Cadenza CW-C18 (50 x 3.0mm)
Cadenza CL-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)

Denatonium benzoate

Application #:
TI0482
Type:
Application Note
Categories:
Food Chemistry
Compounds:
sodium dehydroacetate
potassium sorbate
benzoic acid
Column(s):
Unison UK-Silica (150 x 2.0mm)
Unison UK-Amino (150 x 2.0mm)

Food preservatives (normal phase)

Application #:
TI0481
Type:
Application Note
Categories:
Botanicals
Compounds:
sinigrin
Column(s):
Unison UK-Amino (75 x 2.0mm)

Sinigrin (normal phase)

Application #:
TI0480
Type:
Application Note
Categories:
Botanicals
Compounds:
sulforaphane
allyl isothiocyanate
benzyl isothiocyanate
Column(s):
Cadenza CD-C18 (75 x 2.0mm)
Cadenza CW-C18 (75 x 2.0mm)
Cadenza CL-C18 (75 x 2.0mm)
Unison UK-C18 (75 x 2.0mm)
Unison UK-C8 (75 x 2.0mm)
Unison UK-Phenyl (75 x 2.0mm)

Isothiocyanates of Brassicaceae (Cruciferae)

Application #:
TI0479
Type:
Application Note
Categories:
Botanicals
Compounds:
sinigrin
sulforaphane
allyl isothiocyanate
benzyl isothiocyanate
Column(s):
Cadenza CW-C18 (75 x 2.0mm)
Cadenza CL-C18 (75 x 2.0mm)
Unison UK-C18 (75 x 2.0mm)
Unison UK-C8 (75 x 2.0mm)
Unison UK-Phenyl (75 x 2.0mm)

Isothiocyanates of Brassicaceae (Cruciferae)

Application #:
TI0478
Type:
Application Note
Categories:
Saccharides
Compounds:
meso-erythritol
xylitol
D-fructose
D-sorbitol
D-galactose
D-glucose
sucrose
maltitol
lactose
maltose
trehalose
isomaltose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Comparison with the eluents of acetonitrile and acetone for saccharides separation

Application #:
TI0477
Type:
Application Note
Categories:
Biochemicals and Metabolites
Compounds:
Phosphatidylinositol
Phosphatidylcholine
Phosphatidylethanolamine
Phosphatidylglycerol
Sphingomyelin
Column(s):
Cadenza CW-C18 (100 x 2.0mm)

LC-MS Application for Phospholipids

Application #:
TI0476
Type:
Application Note
Categories:
Column Performance
Compounds:
1-hydroxy-7-azabenzotriazole
acetoaminophen
prednisolone
6-methylprednisolone
methyl-3-amino-2-thiophenecarboxylate
corticosterone
4-aminobenzophenone
propyl paraben
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CW-C18 (150 x 4.6mm)

Substitution of organic solvent for reversed-phase

Application #:
TI0475
Type:
Application Note
Categories:
Column Performance
Compounds:
1-hydroxy-7azabenzotriazole
acetoaminophen
prednisolone
6-methylprednisolone
methyl-3-amino-2-thiophenecarboxylate
corticosterone
4-aminobenzophenone
propyl paraben.
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza 5CD-C18 (150 x 4.6mm)
Cadenza CW-C18 (150 x 3.0mm)

Reducing organic solvent consumption

Application #:
TI0474
Type:
Application Note
Categories:
Aminos Acids
Peptides
Proteins
Compounds:
Phe
Leu
Ile
Trp
Met
Val
Pro
Tyr
Thr
Ala
Arg
Ser
Lys
His
Gly
Asn
gln
Cys
Glu
Asp
Column(s):
Unison UK-Amino (100 x 3.0mm)

Normal Phase Separation for Amino Acids

Application #:
TI0473
Type:
Application Note
Categories:
Amino Acids
Peptides
Proteins
Compounds:
L-phenylalanine
L-tryptophan
L-tyrosine
Column(s):
Unison UK-Amino (250 x 2.0mm)

Aromatic Amino Acids

Application #:
TI0472
Type:
Application Note
Categories:
Amino Acids
Peptides
Proteins
Compounds:
L-Methionine
L-Cysteine
L-Cystine
Column(s):
Unison UK-Amino (250 x 2.0mm)

Sulfur-containing Amino Acids

Application #:
TI0471
Type:
Application Note
Categories:
Amino Acids
Peptides
Proteins
Compounds:
L-Glutamic Acid
L-Aspartic Acid
Column(s):
Unison UK-Amino (50 x 2.0mm)
Unison UK-Amino (250 x 2.0mm)

Acidic Amino Acids

Application #:
TI0470
Type:
Application Note
Categories:
Amino Acids
Peptides
Proteins
Compounds:
L-Threonine
Glycine
L-Glutamine
L-Asparagine
L-Serine
Column(s):
Unison UK-Amino (250 x 2.0mm)

Hydrophilic Amino Acids

Application #:
TI0469
Type:
Application Note
Categories:
Amino Acids
Peptides
Proteins
Compounds:
L-leucine
L-isoleucine
L-proline
L-valine
L-alanine
Glycine
Column(s):
Unison UK-Amino (250 x 2.0mm)

Aliphatic Amino Acids

Application #:
TI0468
Type:
Application Note
Categories:
Saccharides
Compounds:
Sorbitol
Mannose
Mannitol
Glucose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Sorbitol, Mannose, Mannitol, Glucose

Application #:
TI0467
Type:
Application Note
Categories:
Saccharides
Compounds:
D-Mannitol
D-Glucose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Mannitol, Glucose

Application #:
TI0466
Type:
Application Note
Categories:
Biochemicals and Metabolites
Compounds:
Creatinine and Purine Metabolites
Column(s):
Unison UK-Amino (150 x 4.6mm)

Creatinine and Purine Metabolites

Application #:
TI0465
Type:
Application Note
Categories:
Natural Compounds
Compounds:
Lycopene
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CW-C18 (150 x 4.6mm)
Cadenza CL-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Lycopene

Application #:
TI0464
Type:
Application Note
Categories:
Vitamins
Compounds:
25-Hydroxy vitamin D3 (Calcifediol)
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

LC-MS/MS Application for 25-Hydroxy Vitamin D3

Application #:
TI0463
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Compounds:
Duloxetine
Ethyl Morphine
Column(s):
Unison US-C18 (50 x 2.0mm)

LC-MS/MS Application for Duloxetine and Ethyl Morphine in Whole Blood

Application #:
TI0462
Type:
Application Note
Categories:
Oligosaccharides
Biochemistry / Metabolism
Botanicals
Compounds:
Unsaturated Chondro-Disaccharides
Column(s):
Unison UK-Amino (250 x 3.0mm)

Unsaturated Chondro-Disaccharides

Application #:
TI0458
Type:
Application Note
Categories:
Industrial Chemicals
Pharmaceuticals & Cosmetics
Compounds:
ethylenediamine
diethylenetriamine
triethylenetetramine
tetraethylenepentamine
Column(s):
Unison UK-C18 (75 x 3.0mm)

Aliphatic Amines

Application #:
TI0457
Type:
Application Note
Categories:
Industrial Chemicals
Food Chemistry
Environmental and Agricultural
Compounds:
sudan I
sudan II
sudan III
sudan IV
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CW-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Sudan Dyes

Application #:
TI0456
Type:
Application Note
Categories:
Industrial Chemicals
Food Chemistry
Environmental and Agricultural
Compounds:
leucomalachite green
malachite green
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CW-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Malachite Green and Metabolite

Application #:
TI0455
Type:
Application Note
Categories:
Saccharides
Compounds:
D-arabinose
D-xylose
D-mannose D-glucose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Arabinose, Xylose, Mannose, Glucose

Application #:
TI0454
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
adenosine 3'
5'- cyclic monophosphate sodium (cAMP)
guanosine 3'
5'- cyclic monophosphate sodium (cGMP)
adenosine 5'- monophosphate disodium (AMP)
guanosine 5'- monophosphate disodium (GMP)
Column(s):
Unison UK-Amino (100 x 3.0mm)

Cyclic Mononucleotides

Application #:
TI0453
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
methacrylamide
acrylonitrile
acrylamide
acrolein
Column(s):
Cadenza CL-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)
Unison UK-Phenyl (250 x 3.0mm)

Acrylic Monomers

Application #:
TI0452
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
methacrylamide
acrylamide
Column(s):
Cadenza CL-C18 (250 x 3.0mm)
Unison UK-Amino (150 x 3.0mm)

Acrylamide and Methachrylamide

Application #:
TI0451
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
acrylonitrile
acrylamide
acrolein
Column(s):
Unison UK-Phenyl (250 x 3.0mm)
Unison UK-Amino (150 x 3.0mm)

Acrylic Monomers

Application #:
TI0450
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Column Performance
Compounds:
propranolol
Column(s):
Unison US-C18 (150 x 4.6mm)

Propranolol

Application #:
TI0449
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
beta-nicotinamide adenine dinucleotide reduced form
disodium (NADH)
beta-nicotinamide adenine dinucleotide phosphate
reduced form
tetrasodium (NADPH)
beta-nicotinamide adenine dinucleotide phosphate
oxidized form
sodium (NADP)
beta-nicotinamide adenine dinucleotide oxidized form (NAD)
Column(s):
Unison UK-Amino (30 x 3.0mm)

Coenzymes (Nicotinamide adenine dinucleotides)

Application #:
TI0448
Type:
Application Note
Categories:
DNA Related Compounds
Biochemistry / Metabolism
Compounds:
adenosine 5'- triphosphate disodium (ATP)
adenosine 5'- monophosphate disodium (AMP)
adenosine 5'- diphosphate disodium (ADP)
Column(s):
Unison UK-Amino (30 x 3.0mm)

Adenosine Phosphates (AMP, ADP, ATP)

Application #:
TI0447
Type:
Application Note
Categories:
Saccharides
Biochemistry / Metabolism
Compounds:
D-fructose 6-phosphate disodium hydrate (F6P)
D-fructose 1
6-disphosphate trisodium octahydrate (F1
6P)
Column(s):
Unison UK-Amino (100 x 3.0mm)

Sugar Phosphates

Application #:
TI0446
Type:
Application Note
Categories:
Saccharides
Biochemistry / Metabolism
Compounds:
D-mannose 6-phosphate barium hydrate (M6P)
D-glucose 6-phosphate disodium (G6P)
D-fructose 6-phosphate disodium hydrate (F6P)
D-fructose 1-phosphate barium (F1P)
alpha-D-glucose 1-phosphate disodium hydrate (G1P)
alpha-D-galactose 1-phosphate dipotassium pentahydrate (Gal1P)
Column(s):
Unison UK-Amino (250 x 3.0mm)

Sugar Phosphates

Application #:
TI0445
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
Standard PA Sugar Chains
Column(s):
Cadenza CW-C18 (250 x 3.0mm)

Standard PA-Sugar Chains

Application #:
TI0444
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
Standard PA-Sugar Chains
Column(s):
Unison UK-Amino (250 x 3.0mm)

Standard PA-Sugar Chains

Application #:
TI0443
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
Standard PA-Sugar Chains
Column(s):
Unison UK-Amino (100 x 3.0mm)

Standard PA-Sugar Chains

Application #:
TI0442
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
Standard PA-Sugar Chains
Column(s):
Unison UK-Amino (250 x 3.0mm)

Standard PA-Sugar Chains

Application #:
TI0441
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
Standard PA-Sugar Chains
Column(s):
Unison UK-Amino (250 x 3.0mm)

Standard PA-Sugar Chains

Application #:
TI0440
Type:
Application Note
Categories:
Proteins
Compounds:
Human Breast Cancer Cells
Column(s):
Intrada WP-RP (250 x 4.6mm)

DAABD-proteins from breast cancer cells

Application #:
TI0439
Type:
Application Note
Categories:
Saccharides
Compounds:
lactulose
lactose
epilactose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Epilactose, Lactulose, Lactose

Application #:
TI0438
Type:
Application Note
Categories:
Industrial Chemicals
Food Chemistry
LC-MS
Compounds:
melamine
cyanuric acid
ammeline
ammelide
Column(s):
Unison UK-Amino (150 x 3.0mm)

Melamine & related compounds

Application #:
TI0437
Type:
Application Note
Categories:
Industrial Chemicals
Food Chemistry
Compounds:
Melamine
Cyanuric Acid
Column(s):
Unison UK-Amino (150 x 2.0mm)

Melamine & Cyanuric Acid

Application #:
TI0436
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
vancomycin
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CW-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)

Antibiotic (Vancomycin)

Application #:
TI0435
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
tylosin
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CW-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)

Antibiotic (Tylosin)

Application #:
TI0434
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
rifampicin
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CW-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)

Antibiotic (Rifampicin)

Application #:
TI0433
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
2-Amino Benzoic Acid (2-AA) Labeled Oligosaccharides
Column(s):
Unison UK-Amino (250 x 4.6mm)

2-AA labeled Fetuin Oligosaccharides

Application #:
TI0432
Type:
Application Note
Categories:
Saccharides
Botanicals
Pharmaceuticals & Cosmetics
Compounds:
nojirimycin bisulfite
1-deoxynojirimycin hydrochloride
1-deoxymannojirimycin hydrochloride
Column(s):
Unison UK-Amino (30 x 3.0mm)

Nojirimycin Relative Compounds

Application #:
TI0431
Type:
Application Note
Categories:
Saccharides
Botanicals
Pharmaceuticals & Cosmetics
Compounds:
D-glucose
1-deoxynojirimycin hydrochloride
Column(s):
Unison UK-Amino (250 x 3.0mm)

Deoxynojirimycin, Glucose

Application #:
TI0430
Type:
Application Note
Categories:
Proteins
Compounds:
IgG2 mAb
Column(s):
Intrada WP-RP (150 x 2.0mm)

IgG mAb by reversed phase analysis

Application #:
TI0429
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
N-glycolylneuraminic acid
N-acetylneuraminic acid
2-keto-D-gluconic acid hemicalcium salt hydrate
2-keto-3-deoxyoctonate ammonium salt
Column(s):
Unison UK-Amino (250 x 3.0mm)

Alpha-keto acids

Application #:
TI0428
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
erythromycin
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CW-C18 (150 x 4.6mm)

Antibiotic (Erythromycin)

Application #:
TI0427
Type:
Application Note
Categories:
Amino Acids and Peptides
Pharmaceuticals & Cosmetics
Compounds:
Polymyxin B
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CW-C18 (75 x 3.0mm)

Polypeptide Antibiotic (2) - Polymyxin B

Application #:
TI0426
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
tobramycin sulfate
streptomycin sulfate
kanamycin sulfate
gentamicin sulfate
amikacin disulfate
Column(s):
Cadenza CD-C18 (250 x 3.0mm)
Cadenza CW-C18 (250 x 3.0mm)
Cadenza CL-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

Aminoglycoside Antibiotics

Application #:
TI0425
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
microcystin RR
microcystin YR
microcystin LR
Column(s):
Unison US-C18 (50 x 2.0mm)

Microcystins

Application #:
TI0424
Type:
Application Note
Categories:
Botanicals
Food Chemistry
Compounds:
rutinose
Peonidin (Peo)
Pelargonidin (Pel)
Malvidin (Mal)
malonylglucoside (MG)
galactose
Delphinidin (Del)
Cyanidin (Cya)
coumaroylglucoside (CG)
caffeoylglucoside (CafG)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Anthocyanins

Application #:
TI0423
Type:
Application Note
Categories:
Amino Acids and Peptides
Compounds:
NDA (naphthalene-2
3-dicarboxaldehyde)
Column(s):
Cadenza CD-C18 (150 x 2.0mm)

NDA-amino acids

Application #:
TI0422
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
acyclovir
lactose
Column(s):
Unison UK-Amino (50 x 3.0mm)

Acyclovir and Lactose

Application #:
TI0421
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
PQQ ( pyrroloquinoline quinone )
Column(s):
Unison UK-Silica (150 x 2.0mm)
Unison UK-Amino (150 x 2.0mm)

PQQ: pyrroloquinoline quinone (Normal Phase)

Application #:
TI0420
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
microcystin YR
microcystin RR
microcystin LR
Column(s):
Unison UK-Amino (150 x 3.0mm)

Microcystins

Application #:
TI0419
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
microcystin YR
microcystin RR
microcystin LR
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)
Unison UK-Phenyl (150 x 3.0mm)

Microcystins

Application #:
TI0418
Type:
Application Note
Categories:
Column Performance
Compounds:
Tetracaine
Propylbenzoate
Column(s):
Cadenza CW-C18 (0 x 0mm)

pH Durability of Cadenza CW-C18

Application #:
TI0417
Type:
Application Note
Categories:
Biochemistry / Metabolism
Pharmaceuticals & Cosmetics
Compounds:
theophylline
theobromine
caffeine
beta-(7-hydroxyethyl) theophylline
3-propylxanthine
1-methylxanthine
Column(s):
Unison UK-Amino (100 x 3.0mm)

Xanthines and Metabolites

Application #:
TI0416
Type:
Application Note
Categories:
Direct Serum Injection
Biochemistry / Metabolism
Compounds:
theophylline
theobromine
caffeine
beta-(7-hydroxyethyl) theophylline
3-propylxanthine
1-methylxanthine
Column(s):
Cadenza HS-C18 (150 x 3.0mm)

Xanthines and Metabolites in Bovine Serum

Application #:
TI0415
Type:
Application Note
Categories:
Biochemistry / Metabolism
Pharmaceuticals & Cosmetics
Compounds:
theophylline
theobromine
caffeine
beta-(7-hydroxyethyl) theophylline
3-propylxanthine
1-methylxanthine
Column(s):
Cadenza 5CD-C18 (75 x 2.0mm)
Cadenza CW-C18 (75 x 2.0mm)
Unison US-C18 (150 x 4.6mm)

Xanthines and Metabolites

Application #:
TI0414
Type:
Application Note
Categories:
Column Performance
Compounds:
propylparaben
prednisolone
methyl 3-amino-2-thiophenecarboxylate
corticosterone
acetaminophen
6-alpha-methylprednisolone
4-aminobenzophenone
1-hydroxy-7-azabenzotriazole
Column(s):
Cadenza CW-C18 (150 x 4.6mm)

Batch-to-batch reproducibility of CW-C18

Application #:
TI0413
Type:
Application Note
Categories:
Direct Serum Injection
Environmental and Agricultural
Compounds:
PFBS perfluorobutanesulfonic acid
PFOA perfluorooctanoic acid
PFOS perfluorooctanesulfonic acid
Column(s):
Cadenza HS-C18 (250 x 3.0mm)

PFAAs in Bovine Serum

Application #:
TI0412
Type:
Application Note
Categories:
Environmental and Agricultural
Industrial Chemicals
Compounds:
perfluorooctanoic acid
perfluorooctanesulfonic acid
perfluorobutanesulfonic acid
Column(s):
Cadenza CD-C18 (75 x 2.0mm)
Cadenza CW-C18 (75 x 2.0mm)
Cadenza CL-C18 (75 x 2.0mm)
Unison UK-C18 (75 x 2.0mm)
Unison UK-C8 (75 x 2.0mm)
Unison UK-Phenyl (75 x 2.0mm)

PFAAs

Application #:
TI0411
Type:
Application Note
Categories:
LC-MS
Pharmaceuticals & Cosmetics
Compounds:
tetracycline
oxytetracycline
chlortetracycline
Column(s):
Cadenza CW-C18 (100 x 2.0mm)

LC-MS/MS Application -Tetracycline Antibiotics

Application #:
TI0410
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
tetracycline (TC)
oxytetracycline (OTC)
chlortetracycline (CTC)
Column(s):
Cadenza CW-C18 (150 x 4.6mm)

Tetracycline antibiotics

Application #:
TI0409
Type:
Application Note
Categories:
Biochemistry / Metabolism
Pharmaceuticals & Cosmetics
Compounds:
uracil
dihydrouracil
dihydrofluorouracil
5-fluorouracil
Column(s):
Unison UK-Phenyl (250 x 4.6mm)
Unison UK-Amino (250 x 3.0mm)

5-Fluorouracil and Related Compounds

Application #:
TI0408
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
Inulin type oligosaccharides
Column(s):
Unison UK-Amino (250 x 3.0mm)

Inulin type oligosaccharides

Application #:
TI0407
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
6-beta-D-fructopyranosyl glucose
2G-alpha-D-glucopyranosyl stachyose
2G-alpha-D-glucopyranosyl raffinose
2G-alpha-D-glucopyranosyl platinose
2G(2-alpha-D-glucopyranosyl)3 stachyose
2G(2-alpha-D-glucopyranosyl)3 raffinose
2G(2-alpha-D-glucopyranosyl)2 stachyose
2G(2-alpha-D-glucopyranosyl)2 raffinose
2-alpha-D-glucopyranosyl isokestose
2-alpha-D-glucopyranosyl fructosylxyloside
2(2-alpha-D-glucopyranosyl)3 isokestose
2(2-alpha-D-glucopyranosyl)2 nystose
2(2-alpha-D-glucopyranosyl)2 isokestose
1F-beta-D-fructofuranosyl stachyose
1F-beta-D-fructofuranosyl raffinose
1F-beta-D-fructofuranosyl lactosucrose
1F(1-beta-D-fructofuranosyl)2 lactosucrose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Fructooligosaccharides

Application #:
TI0406
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
uridine
uracil
thymine
thymidine
guanosine
guanine
cytosine
cytidine
adenosine
adenine
Column(s):
Unison UK-Amino (250 x 3.0mm)

Nucleosides and Bases

Application #:
TI0405
Type:
Application Note
Categories:
Saccharides
Compounds:
trehalulose
trehalose
sucrose
palatinose
maltose
maltitol
lactose
lactitol
isomaltose
D-sorbitol
D-glucose
D-fructose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Mono-, Disaccharides and Sugar alcohols(2)

Application #:
TI0404
Type:
Application Note
Categories:
Saccharides
Compounds:
D-xylose
D-mannose
D-glucose
D-galactose
D-arabinose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Arabinose, Xylose, Mannose, Galactose, Glucose

Application #:
TI0403
Type:
Application Note
Categories:
Industrial Chemicals
Food Chemistry
Pharmaceuticals & Cosmetics
Compounds:
n-propyl gallate
nordihydroguaiaretic acid
n-octyl gallate
n-dodecyl gallate
butylhydroxytoluene
butylhydroquinone
4-hydroxy-3-t-butylanisole
2
6-di-tert-butyl-4-hydroxymethylphenol
Column(s):
Cadenza CD-C18 (50 x 2.0mm)
Cadenza 5CD-C18 (150 x 4.6mm)
Cadenza 5CD-C18 (150 x 4.6mm)

Antioxidants

Application #:
TI0402
Type:
Application Note
Categories:
Saccharides
Pharmaceuticals & Cosmetics
Oligosaccharides
Compounds:
voglibose
acarbose
Column(s):
Unison UK-Amino (250 x 3.0mm)
Unison UK-Amino (250 x 4.6mm)

Voglibose, Acarbose

Application #:
TI0401
Type:
Application Note
Categories:
Column Performance
Compounds:
Phenylalkanes
Column(s):
Cadenza CD-C18 (0 x 0mm)
Cadenza CW-C18 (0 x 0mm)
Unison UK-C8 (0 x 0mm)
Unison UK-Phenyl (0 x 0mm)

Retention and hydrophobic properties of Cadenza CW-C18

Application #:
TI0400
Type:
Application Note
Categories:
Column Performance
Compounds:
triphenylene
phenol
o-terphenyl
caffeine
butylbenzene
amylbenzene
Column(s):
Cadenza CW-C18 (0 x 0mm)

Basic Interaction of Cadenza CW-C18

Application #:
TI0399
Type:
Application Note
Categories:
Amino Acids and Peptides
Pharmaceuticals & Cosmetics
Compounds:
bacitracin
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CW-C18 (75 x 4.6mm)

Polypeptide Antibiotic, bacitracin

Application #:
TI0398
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
trimipramine
nortriptyline
imipramine
doxepine
desipramine
amitriptyline
Column(s):
Cadenza CW-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)

Basic Compounds: Antidepressant drug

Application #:
TI0397
Type:
Application Note
Categories:
LC-MS
Steroids
Biochemistry / Metabolism
Compounds:
testosterone
progesterone
pregnanetriol
pregnanediol
estrone
estriol
5-alpha-dihydrotestosterone
17-beta-estradiol
17-alphahydroxyprogesterone
Column(s):
Unison UK-C8 (50 x 2.0mm)

LC-MS/MS Application - Steroid Hormones and Metabolites

Application #:
TI0396
Type:
Application Note
Categories:
LC-MS
Environmental and Agricultural
Compounds:
methamidophos
dichlorvos
Column(s):
Unison UK-C18 (50 x 2.0mm)

LC-MS/MS Application - Organophosphorous Pesticides

Application #:
TI0395
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
polyvinylpyrrolidone K30 (PVP)
disopyramide
Column(s):
Cadenza HS-C18 (30 x 3.0mm)

Disopyramide in PVP Aqueous Solution

Application #:
TI0394
Type:
Application Note
Categories:
Vitamins
Compounds:
nicotinic acid
Column(s):
Unison UK-Amino (100 x 2.0mm)

The Unison UK-Amino column shows excellent retention of nicotinic acid in both normal phase and anion exchange modes.

Application #:
TI0393
Type:
Application Note
Categories:
Amino Acids and Peptides
Biochemistry / Metabolism
Compounds:
glutathione reduced (GSH)
glutathione oxidized (GSSG)
Column(s):
Unison UK-Amino (100 x 4.6mm)

The Unison UK-Amino column shows excellent recognition of both the oxidized and reduced forms of glutathione.

Application #:
TI0392
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
verapamil hydrochloride
hydroxypropylcellulose (HPC)
Column(s):
Cadenza HS-C18 (30 x 3.0mm)

The Cadenza HS-C18 column shows excellent exclusion of the large polymer HPC from the main sharp peak, verapamil.

Application #:
TI0391
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
cefalexin
carboxymethylcellulose sodium salt (CMC-Na)
Column(s):
Cadenza HS-C18 (30 x 3.0mm)

Cefalexin in CMC-Na Aqueous Solution

Application #:
TI0390
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
lidocaine
carboxymethylcellulose sodium salt (CMC-Na)
Column(s):
Cadenza HS-C18 (30 x 3.0mm)

Lidocaine in CMC-Na Aqueous Solution

Application #:
TI0389
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
sulfamethoxazole
sodium alginate
Column(s):
Cadenza HS-C18 (30 x 3.0mm)

Sulfamethoxazole in Alginate Aqueous Solution

Application #:
TI0388
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
calcitonin acetate
salmon (MW 3492)
Column(s):
Cadenza CD-C18 (100 x 3.0mm)
Cadenza CW-C18 (100 x 3.0mm)
Cadenza CL-C18 (100 x 3.0mm)
Unison UK-C18 (100 x 3.0mm)
Unison UK-Amino (100 x 3.0mm)

Calcitonin, salmon

Application #:
TI0387
Type:
Application Note
Categories:
Amino Acids and Peptides
Pharmaceuticals & Cosmetics
Compounds:
calcitonin acetate
salmon (MW 3492)
Column(s):
Cadenza HS-C18 (30 x 3.0mm)

Calcitonin in Gelatin Aqueous Solution

Application #:
TI0386
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
indomethacin
Column(s):
Cadenza HS-C18 (30 x 3.0mm)

Indomethacin in gelatin aqueous solution

Application #:
TI0385
Type:
Application Note
Categories:
Column Performance
Compounds:
uracil
toluene
naphthalene
fluorene
benzene
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

HT performance with wide-pore ODS phase

Application #:
TI0384
Type:
Application Note
Categories:
Proteins
Compounds:
Tween20
Column(s):
Cadenza HS-C18 (50 x 3.0mm)

Tween 20 in IgG solution

Application #:
TI0383
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
trichloroacetic acid (TCAA)
tribromoacetic acid (TBAA)
monochloroacetic acid (MCAA)
monobromoacetic acid (MBAA)
dichloroacetic acid (DCAA)
dibromoacetic acid (DBAA)
bromochloroacetic acid (BCAA)
Column(s):
Cadenza CW-C18 (250 x 3.0mm)
Unison UK-Amino (250 x 3.0mm)

Halo acetic acids

Application #:
TI0382
Type:
Application Note
Categories:
Saccharides
Compounds:
sucrose
maltose
lactose
Column(s):
Unison UK-Amino (250 x 4.6mm)

Sucrose, lactose, maltose

Application #:
TI0381
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
polyethylene glycol #6000
polyethylene glycol #2000
polyethylene glycol #12000
Column(s):
Cadenza CW-C18 (150 x 4.6mm)
Unison UK-Amino (150 x 4.6mm)

Polyethylene glycol # 2000, 6000, 12000

Application #:
TI0380
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
polyethylene glycol #2000
Column(s):
Cadenza CW-C18 (250 x 4.6mm)
Unison UK-Amino (250 x 4.6mm)

PEG can be separated by both reversed phase and normal phase chromatography. Reversed phase using the Cadenza CW-C18 column gives baseline resolution as well as better run time.

Application #:
TI0379
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
nicotine
cotinine
anabasine hydrochloride
3-hydroxycotinine
Column(s):
Cadenza CD-C18 (100 x 3.0mm)
Unison UK-Amino (100 x 3.0mm)

Nicotine derivatives and metabolites can be separated by both reversed phase and normal phase chromatography. Reversed phase gives better run time.

Application #:
TI0378
Type:
Application Note
Categories:
Food Chemistry
Compounds:
sodium sulfamate (aminosulfonic acid sodium salt)
cyclamic acid (N-cyclohexylsulfamic acid)
? cyclamic acid (N-cyclohexylsulfamic acid)
Column(s):
Unison UK-C18 (50 x 3.0mm)
Unison UK-Amino (50 x 3.0mm)

Sulfamic acid and cyclamic acid can be separated by either reversed phase or normal phase chromatography. Reversed phase gives better peak shape while normal phase gives better run time.

Application #:
TI0377
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
L-carnitine
GABA
betaine
Column(s):
Unison UK-Amino (50 x 3.0mm)

Unison UK-Amino is able to separate amino acid-like molecules with good resolution.

Application #:
TI0376
Type:
Application Note
Categories:
Direct Serum Injection
Steroids
Biochemistry / Metabolism
Compounds:
beta-Estradiol 3
17-disulfate
beta-Estradiol 17-(beta-D-glucuronide)
17-beta-estradiol
Column(s):
Cadenza HS-C18 (150 x 3.0mm)

Cadenza HS-C18 successfully separates out small molecules like Estradiol and metabolites from bovine serum. The sample chromatogram looks almost identical to the standard chromatogram.

Application #:
TI0375
Type:
Application Note
Categories:
Steroids
Biochemistry / Metabolism
Compounds:
beta-Estradiol 3
17-disulfate
beta-Estradiol 17-(beta-D-glucuronide)
17-beta-estradiol
Column(s):
Cadenza CD-C18 (30 x 2.0mm)
Cadenza CW-C18 (30 x 2.0mm)
Cadenza CL-C18 (30 x 2.0mm)
Unison UK-C18 (30 x 2.0mm)
Unison UK-C8 (30 x 2.0mm)
Unison UK-Phenyl (30 x 2.0mm)

Many Cadenza and Unison columns achieve excellent resolution with beta-Estradiol 3,17-disulfate and 17beta-estradiol despite their similar structures.

Application #:
TI0374
Type:
Application Note
Categories:
Amino Acids and Peptides
Compounds:
angiotension IV
angiotension III
angiotension II
angiotension I (human)
angiotension I (bovine)
Column(s):
Unison UK-C18 (150 x 3.0mm)
Unison UK-Amino (150 x 3.0mm)

Unison UK-Amino separates Angiotensin III and Angiotensisn II better than a standard reverse phased column.

Application #:
TI0373
Type:
Application Note
Categories:
Saccharides
Food Chemistry
Compounds:
sucralose
saccharin
aspartame
acesulfame
Column(s):
Unison UK-Amino (250 x 3.0mm)

Unison UK-Amino column separates small molecule low-calorie sweeteners with excellent resolution.

Application #:
TI0372
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
cefdinir
cefalexin
cefaclor
Column(s):
Cadenza CD-C18 (50 x 3.0mm)
Unison UK-Amino (50 x 3.0mm)

Unison UK-Amino and Cadenza CD-C18 provide different selectivity for cephem antibiotics due to the difference in the stationary phase.

Application #:
TI0371
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
propyl-L-arginine (N-PLA)
monomethyl-L-arginine (L-NMMA)
dimetyl-L-arginine (SDMA)
dimetyl-L-arginine (ADMA)
Column(s):
Unison UK-C18 (150 x 4.6mm)

Unison UK-C18 successfully separates methylated arginines of similar structure. Compounds are pre-column derivatized to enable fluroescence detection.

Application #:
TI0370
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
6-nitronorepine
6-nitroepinephrine
6-nitrodopamine
Column(s):
Unison UK-C18 (250 x 4.6mm)

Unison UK-C18 successfully separates nitroNE, nitroE, and nitroDA. Detection is via post-column derivatization.

Application #:
TI0369
Type:
Application Note
Categories:
Botanicals
Compounds:
quercetin-3-gluoronide
quercetin-3-glucoside
Column(s):
Unison UK-Amino (250 x 4.6mm)

The Unison UK-Amino column selectively separates Q3G and Q3GA although they are almost identical in structure.

Application #:
TI0368
Type:
Application Note
Categories:
Botanicals
Compounds:
quercetin
myricetin
luteolin
kdempferol
fisetin
apigenin
Column(s):
Unison UK-Amino (250 x 4.6mm)

The Unison UK-Amino column simulateously analyzes flavenoids of similar structure. The hydroxyl groups on the molecules interact with the stationary phase.

Application #:
TI0367
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
victoria blue
patent blue VF
patent blue V
pararosaniline
methyl violet
crystal violet
Column(s):
Cadenza CW-C18 (75 x 3.0mm)

The Cadenza CW-C18 column effectively separates triarylmethane ink dyes because of chelating properties.

Application #:
TI0366
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
Double-Stranded DNA Fragments
Column(s):
Cadenza CW-C18 (250 x 4.6mm)
Cadenza CL-C18 (250 x 4.6mm)

Cadenza CW-C18 and Cadenza CL-C18 have different selectivities for double-stranded DNA. CW-C18 is a wide pore column that elutes the molecules earlier than the CL-C18, which is selective via its exposed silanol groups.

Application #:
TI0365
Type:
Application Note
Categories:
LC-MS
Pharmaceuticals & Cosmetics
Compounds:
oxymorphone
oxycodone
morphrine
hydromorphone
hydrocodone
codeine
6-acetyl morphine
Column(s):
Unison US-C18 (50 x 2.0mm)

Opiates of similar structure can be separated and analyzed by LC-MS. For example, morphine and oxymorphone are very similar but are resolved into two separate peaks.

Application #:
TI0364
Type:
Application Note
Categories:
Saccharides
Compounds:
maltose
maltitol
cellobiose
cellobiitol
Column(s):
Unison UK-Amino (250 x 3.0mm)

When using the Unison UK-Amino column, THF can better separate cellobiose and cellobiitol than acetonitrile.

Application #:
TI0363
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
mononucleotides
Column(s):
Unison UK-Amino (250 x 4.6mm)

Unison UK-Amino separates mononucleotides of similar structure. For example, dGMP and GMP are resolved even though they differ in only a single hydroxyl group.

Application #:
TI0362
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
norepinephrine
epinephrine
dopamine
Column(s):
Unison UK-Amino (30 x 3.0mm)

Unison UK-Amino column separates catecholamines of similar structure using normal phase/anion exchange mode.

Application #:
TI0361
Type:
Application Note
Categories:
Saccharides
Food Chemistry
Compounds:
lnositol in nutritious supplement drink
Column(s):
Unison UK-Amino (100 x 3.0mm)

Unison UK-Amino is able to separate inositol (an acidic compound) in normal phase/anion exchange mode. This column recognizes inositol from a mixture containing a variety of components.

Application #:
TI0360
Type:
Application Note
Categories:
Biochemistry / Metabolism
Food Chemistry
Compounds:
Taurine in nutritious supplement drink
Column(s):
Unison UK-Amino (100 x 3.0mm)

Unison UK-Amino column separates out taurine in a drink mixture.

Application #:
TI0359
Type:
Application Note
Categories:
Vitamins
Compounds:
pyridoxine
pyridoxal
Column(s):
Unison UK-Amino (250 x 4.6mm)

Vitamin B6

Application #:
TI0358
Type:
Application Note
Categories:
Saccharides
Compounds:
L-sorbitol
L-psicose
L-mannitol
L-fructose
D-talitol
D-tagatose
D-sorbose
D-psicose
allitol
Column(s):
Unison UK-Amino (250 x 3.0mm)

Rare sugars

Application #:
TI0357
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
vanillylmandelic acid (VMA)
mandelic acid
homovanillic acid (HVA)
Column(s):
Unison UK-Amino (150 x 4.6mm)

Mandelic acid and related compounds

Application #:
TI0356
Type:
Application Note
Categories:
Botanicals
Food Chemistry
Compounds:
stevioside
rebaudioside A
Column(s):
Unison UK-Amino (150 x 2.0mm)

Stevia sweeteners

Application #:
TI0355
Type:
Application Note
Categories:
Botanicals
Compounds:
sandalwood
peppermint
lavender
grapefruit
eucalyptus
Column(s):
Cadenza CD-C18 (500 x 4.6mm)

Essential oils

Application #:
TI0354
Type:
Application Note
Categories:
Botanicals
Compounds:
peppermint
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Cadenza CD-C18 (500 x 4.6mm)

Essential oils (peppermint)

Application #:
TI0353
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
xylotriose
xylose
xylobiose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Xylooligosaccharides

Application #:
TI0352
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
4'-galactosyllactose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Galactooligosaccharides

Application #:
TI0351
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
nystose
frutofuranosylnystose
1-kestose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Fructooligosaccharides

Application #:
TI0350
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
gentiotriose
gentiotetraose
gentiobiose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Gentiooligosaccharides

Application #:
TI0349
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
isomaltotroise
isomaltose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Isomaltooligosaccharides

Application #:
TI0348
Type:
Application Note
Categories:
Food Chemistry
Compounds:
tartaric acid
succinic acid
propionic acid
malonic acid
lactic acid
fumaric acid
formic acid
citric acid
acetic acid
Column(s):
Cadenza CL-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Organic acids

Application #:
TI0347
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
maltooligosaccharide
Column(s):
Unison UK-C18 (250 x 3.0mm)
Unison UK-Amino (250 x 3.0mm)

Maltooligosaccharides

Application #:
TI0346
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
D-glucosamine
chitosan trimer
chitosan tetramer
chitosan pentamer
chitosan hexamer
chitosan dimer
Column(s):
Unison UK-C18 (150 x 3.0mm)
Unison UK-Amino (250 x 3.0mm)

Chitosanoligosaccharides

Application #:
TI0345
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
p-methylhippuric acid
o-methylhippuric acid
m-methylhippuric acid
hippuric acid
Column(s):
Unison UK-Amino (250 x 4.6mm)

Hippuric acids

Application #:
TI0344
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
surfactant (lgepal)
Column(s):
Cadenza CL-C18 (250 x 4.6mm)
Unison UK-Amino (250 x 4.6mm)

Igepal CO-720

Application #:
TI0343
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
surfactant (triton)
Column(s):
Cadenza CL-C18 (250 x 4.6mm)
Unison UK-Amino (250 x 4.6mm)

Triton X-100

Application #:
TI0342
Type:
Application Note
Categories:
Saccharides
Compounds:
6-sialyllactose
3-sialyllactose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Sialyllactoses

Application #:
TI0341
Type:
Application Note
Categories:
Vitamins
Compounds:
L-ascorbic acid (Vitamin C)
isoascorbic acid (erythorbic acid)
dehydroascorbic acid
Column(s):
Unison UK-Amino (150 x 2.0mm)

Dehydroascorbic acid and related compounds

Application #:
TI0340
Type:
Application Note
Categories:
Botanicals
Compounds:
wogonin
sutellarein
epigallocatechin gallate
epigallocatechin
epicatechin gallate
epicatechin
catechin
baicalin
baicalein
Column(s):
Unison UK-C18 (250 x 4.6mm)

Catechins and flavonoids

Application #:
TI0339
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
creatinine
creatine phosphate
creatine
Column(s):
Unison UK-Amino (150 x 2.0mm)

Creatine and metabolites

Application #:
TI0338
Type:
Application Note
Categories:
Biochemistry / Metabolism
LC-MS
Compounds:
delta-9tetrahydrocannabinol
11-hydroxy-delta-9-tetrahydrocannabinol
Column(s):
Unison US-C18 (50 x 2.0mm)

LC-MS of marajuana and its metabolite in blood

Application #:
TI0337
Type:
Application Note
Categories:
Saccharides
Compounds:
raffinose
panose
maltotriose
lactosucrose
isomaltotriose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Trisaccharides

Application #:
TI0336
Type:
Application Note
Categories:
Saccharides
Compounds:
sucrose
palatinose
maltulose
D-turanose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Sucrose isomers

Application #:
TI0335
Type:
Application Note
Categories:
Saccharides
Compounds:
L-rhamnose
D-mannose
D-glucose
D-galactose
D-fucose
D-fructose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Hexoses

Application #:
TI0334
Type:
Application Note
Categories:
Saccharides
Compounds:
N-acetyl-D-mannosamine
N-acetyl-D-glucosamine
N-acetyl-D-galactosamine
D-mannosamine hydrochloride
D-glucosamine hydrochloride
D-galactosamine hydrochloride
Column(s):
Unison UK-Amino (250 x 3.0mm)

Amino sugars

Application #:
TI0333
Type:
Application Note
Categories:
Saccharides
Biochemistry / Metabolism
Compounds:
N-acetylneuraminic acid (sialic acid)
D-glucuronic acid
D-galacturonic acid
Column(s):
Unison UK-Amino (250 x 3.0mm)

Sialic acid and uronic acids

Application #:
TI0332
Type:
Application Note
Categories:
Saccharides
Compounds:
sodiuum gluconate
D-glucose
D-glucono-1
5-lactone
D-gluconic acid delta lactone
Column(s):
Unison UK-Amino (250 x 3.0mm)

Glucose and its oxidents

Application #:
TI0331
Type:
Application Note
Categories:
Industrial Chemicals
Food Chemistry
Compounds:
stearic acid
palmitic acid
myristic acid
lauric acid
arachidic acid
Column(s):
Cadenza CD-C18 (250 x 3.0mm)
Unison UK-Amino (250 x 3.0mm)

Fatty acids

Application #:
TI0330
Type:
Application Note
Categories:
Vitamins
Compounds:
Vitamin KI
Vitamin K3
Vitamin E
Vitamin D3
Vitamin D2
Vitamin A acetate
Vitamin A
Column(s):
Unison UK-Amino (250 x 3.0mm)
Unison UK-Amino (250 x 4.6mm)

Fat soluble vitamins

Application #:
TI0329
Type:
Application Note
Categories:
Amino Acids and Peptides
Compounds:
L-proline
L-lysine
L-histidine
L-arginine
Column(s):
Unison UK-Amino (250 x 4.6mm)

Basic amino acids

Application #:
TI0328
Type:
Application Note
Categories:
Saccharides
Compounds:
D-xylose
D-ribose
D-lyxose
D-arabinose
2-deoxy-D-ribose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Pentoses

Application #:
TI0327
Type:
Application Note
Categories:
Saccharides
Compounds:
trehalose
sophorose
nigerose
maltose
laminaribiose
kojibiose
isomaltose
gentiobiose
cellobiose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Glucose disaccharide isomers

Application #:
TI0326
Type:
Application Note
Categories:
Steroids
Compounds:
testosterone
progesterone
estrone
estriol
cholesterol
17 beta-estradiol
Column(s):
Unison UK-Phenyl (100 x 2.0mm)

Cholesterol and steroid hormones

Application #:
TI0325
Type:
Application Note
Categories:
Saccharides
Compounds:
xylitol
trehalose
sucrose
meso-erythritol
maltose
maltitol
lactose
isomaltose
D-sorbitol
D-glucose
D-galactose
D-fructose
Column(s):
Unison UK-Amino (250 x 3.0mm)

Mono, disaccharides, and sugar alcohols

Application #:
TI0324
Type:
Application Note
Categories:
Steroids
Compounds:
progesterone
prednisone
prednisolone
hydrocortisone
ethynylestradiol
estrone
estriol
estradiol
deoxycorticosterone acetate
cortisone
corticosterone
6 alpha-methylprednisoline
Column(s):
Cadenza CL-C18 (250 x 4.6mm)

Steroids

Application #:
TI0323
Type:
Application Note
Categories:
Steroids
Compounds:
progesterone
prednisone
prednisolone
hydrocortisone
ethynylestradiol
estrone
estriol
estradiol
deoxycorticosterone acetate
cortisone
corticosterone
6 alpha-methylprednisoline
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Cadenza CL-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)
Unison UK-C8 (250 x 4.6mm)
Unison UK-Phenyl (250 x 4.6mm)

Steroids

Application #:
TI0322
Type:
Application Note
Categories:
Saccharides
Compounds:
D-glucose
D-galactose
Column(s):
Unison UK-Amino (250 x 4.6mm)

Galactose and glucose

Application #:
TI0321
Type:
Application Note
Categories:
Saccharides
Compounds:
sucrose
lactose
Column(s):
Unison UK-Amino (250 x 4.6mm)

Sucrose and lactose in cafe au lait

Application #:
TI0320
Type:
Application Note
Categories:
Saccharides
Compounds:
maltitol
D-galactose
D-arabinose
Column(s):
Unison UK-Amino (250 x 4.6mm)

Arabinose, galactose, maltitol

Application #:
TI0319
Type:
Application Note
Categories:
Steroids
Compounds:
progesterone
prednisone
prednisolone
hydrocortisone
ethylestradiol
estrone
estriol
estrandiol
deoxyrticosterone acetate
cortisone
corticosterone
6 alpha-methylprednisoline
Column(s):
Unison UK-C18 (250 x 4.6mm)
Unison UK-Amino (250 x 4.6mm)

Steroids

Application #:
TI0318
Type:
Application Note
Categories:
Column Performance
Compounds:
uridine
thymidine
propylbenzoate
benzoic acid
3-hydroxyphenylurea
Column(s):
Unison UK-Amino (150 x 2.0mm)

Unison UK-Amino batch to batch reproducibility

Application #:
TI0317
Type:
Application Note
Categories:
Vitamins
Compounds:
L-ascorbic acid (Vitamin C)
isoascorbic acid (erythorbic acid)
Column(s):
Unison UK-Amino (150 x 2.0mm)
Unison UK-Amino (250 x 2.0mm)

Ascorbic and and isoascorbic acid

Application #:
TI0316
Type:
Application Note
Categories:
Column Performance
Compounds:
L-ascorbic acid (Vitamin C)
isoascorbic acid (erythorbic acid)
Column(s):
Unison UK-Amino (250 x 2.0mm)

Normal phase and anion exchange mode

Application #:
TI0315
Type:
Application Note
Categories:
Saccharides
Compounds:
maltose
maltitol
D-sorbitol
D-glucose
Column(s):
Unison UK-Amino (250 x 4.6mm)

Sorbitol, glucose, malitol, maltose

Application #:
TI0313
Type:
Application Note
Categories:
Compounds:
D-sorbitol
D-mannitol
Column(s):
Unison UK-Amino (250 x 2.0mm)
Unison UK-Amino (250 x 4.6mm)

Application #:
TI0313
Type:
Application Note
Categories:
Saccharides
Compounds:
D-sorbitol
D-mannitol
Column(s):
Unison UK-Amino (250 x 2.0mm)
Unison UK-Amino (250 x 4.6mm)

Sugar Alcohols

Application #:
TI0312
Type:
Application Note
Categories:
Vitamins
LC-MS
Compounds:
thiamine
riboflavin
pyridoxine
pantothenic acid
nicotinamide
Folic acid
biotin
ascorbic acid
Column(s):
Unison UK-C18 (250 x 2.0mm)

LC-MS/MS Water Soluble Vitamins

Application #:
TI0311
Type:
Application Note
Categories:
Amino Acids and Peptides
Compounds:
L-valine
L-leucine
L-isoleucine
Column(s):
Unison UK-Amino (150 x 2.0mm)

Branch chained amino acids

Application #:
TI0310
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
β-cyclodextrin
r-cyclodextrin
a-cyclodextrin
Column(s):
Unison UK-Amino (250 x 2.0mm)

Cyclodextrins

Application #:
TI0309
Type:
Application Note
Categories:
Vitamins
Compounds:
thiamine
riboflavin
nicotinic acid
nicotinamide
L-ascorbic acid
Folic acid
cyanocobalamin
Column(s):
Unison UK-Amino (100 x 4.6mm)

Water soluble vitamins

Application #:
TI0308
Type:
Application Note
Categories:
Saccharides
Compounds:
xylitol
myo-inositol
meso-erythritol
maltitol
D-sorbitol
Column(s):
Unison UK-Amino (250 x 2.0mm)

Sugar alcohols

Application #:
TI0307
Type:
Application Note
Categories:
Vitamins
Compounds:
β-tocopherol
y-tocopherol
s-tocopherol
a-tocopherol
Column(s):
Unison UK-Amino (250 x 4.6mm)

Tocopherols

Application #:
TI0306
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
laminaritriose (n=1)
laminaritetraose (n=2)
laminaripentaose (n=3)
laminarihexaose (n=4)
laminariheptaose (n=5)
laminaribiose (n=0)
glucose
Column(s):
Unison UK-Amino (250 x 2.0mm)

Laminariorigosaccharides

Application #:
TI0305
Type:
Application Note
Categories:
Saccharides
Compounds:
xylose
rhamnose
mannose
glucose
fructose
Column(s):
Unison UK-Amino (250 x 2.0mm)

Monosaccharides

Application #:
TI0304
Type:
Application Note
Categories:
Saccharides
Column Performance
Compounds:
sucrose
maltose
glucose
frutose
Column(s):
Unison UK-Amino (250 x 2.0mm)

Unison UK-Amino aqueous durability

Application #:
TI0303
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
minoxidil sulfate
minoxidil
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CW-C18 (150 x 4.6mm)
Cadenza CL-C18 (150 x 4.6mm)
Cadenza HS-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)
Unison UK-C8 (150 x 4.6mm)
Unison UK-Phenyl (150 x 4.6mm)

Minoxidil and metabolite

Application #:
TI0302
Type:
Application Note
Categories:
Direct Serum Injection
Compounds:
minoxidil sulfate
minoxidil
Column(s):
Cadenza HS-C18 (150 x 4.6mm)

Minoxidil and metabolite in bovine serum

Application #:
TI0301
Type:
Application Note
Categories:
Industrial Chemicals
Column Performance
Compounds:
technical seperation of anion surfactants that do not recognize linear chain or multi-branch
Column(s):
Unison UK-Phenyl (150 x 4.6mm)

Anion surfactants

Application #:
TI0300
Type:
Application Note
Categories:
Industrial Chemicals
Column Performance
Compounds:
Aniomic Surfactants (Alkylbensene sulfonates)
Column(s):
Cadenza CD-C18 (250 x 4.6mm)

Anion surfactants

Application #:
TI0299
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
Anions
Column(s):
Cadenza CW-C18 (150 x 4.6mm)
Cadenza CL-C18 (150 x 4.6mm)
Cadenza HS-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)
Unison UK-C8 (150 x 4.6mm)
Unison UK-Phenyl (150 x 4.6mm)

Anions

Application #:
TI0298
Type:
Application Note
Categories:
Environmental and Agricultural
Compounds:
Anions
Column(s):
Unison UK-C18 (150 x 4.6mm)

Anions

Application #:
TI0297
Type:
Application Note
Categories:
Direct Serum Injection
Compounds:
thiamine
nicotinic acid
nicotinamide
L-ascorbic acid
Column(s):
Cadenza HS-C18 (150 x 3.0mm)

Water soluble vitamins in bovine serum

Application #:
TI0296
Type:
Application Note
Categories:
Direct Serum Injection
Compounds:
primidon
phenobarbital
carbamazepin
Column(s):
Cadenza HS-C18 (150 x 3.0mm)

Antieptileptics in bovine serum

Application #:
TI0295
Type:
Application Note
Categories:
Direct Serum Injection
Compounds:
digoxin
digitoxin
Column(s):
Cadenza HS-C18 (150 x 3.0mm)

Digitalis glycosides in bovine serum

Application #:
TI0294
Type:
Application Note
Categories:
Botanicals
Compounds:
quercitrin
quercetin
isoquercitrin
ellagic acid
chlorogenic acid
caffeic acid
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Cadenza HS-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Polyphenol, berry

Application #:
TI0293
Type:
Application Note
Categories:
Proteins
Compounds:
bovine milk
Column(s):
Intrada WP-RP (250 x 3.0mm)

Bovine milk

Application #:
TI0292
Type:
Application Note
Categories:
Food Chemistry
Compounds:
Caffeine in the Caffe Latte
Column(s):
Cadenza HS-C18 (250 x 3.0mm)

Caffeine in the Caffe Latte

Application #:
TI0291
Type:
Application Note
Categories:
Amino Acids and Peptides
Compounds:
Peptides
Tryptic Digest of Hemoglobin
Column(s):
Intrada WP-RP (250 x 3.0mm)
Cadenza CD-C18 (250 x 3.0mm)

Hemoglobin (human) tryptic digest

Application #:
TI0290
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
xanthine oxidase
thyroglobulin
papain
lysozyme
lactferrin
insulin
hemoglobin (human)
hemoglobin (bovine)
deoxyribonclease
cytochrome ( c )
beta-glucosidase
apha-lactalbumin
angiotension II
alpha-chymotryosinogen (bovine pancreas)
albumin (human serum)
albumin (eggs)
albumin (bovine)
Column(s):
Cadenza HS-C18 (150 x 4.6mm)

Protein exclusion on Cadenza HS-C18

Application #:
TI0289
Type:
Application Note
Categories:
Proteins
Compounds:
xanthine oxidase
thyroglobulin
papain
lysozyme
lactferrin
insulin
hemoglobin (Human serum)
hemoglobin (bovine)
deoxyribonclease
cytochrome ( c )
beta-glucosidase
apha-lactalbumin
angiotension II
alpha-chymotryosinogen
albumin (human serum)
albumin (eggs)
albumin (bovine)
Column(s):
Intrada WP-RP (150 x 4.6mm)

Proteins

Application #:
TI0288
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
verapamil
progesterone
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CL-C18 (150 x 4.6mm)
Cadenza HS-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Verapamil

Application #:
TI0286
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
propylbenzene
fluorene
biphenyl
anthracene
Acenaphthene
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Cadenza HS-C18 (250 x 4.6mm)

PAHs

Application #:
TI0285
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
triphenylene
terphenyl (p-)
terphenyl (o-)
terphenyl (m-)
biphenyl
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Cadenza HS-C18 (250 x 4.6mm)

Terphenyl related compounds

Application #:
TI0284
Type:
Application Note
Categories:
Environmental and Agricultural
Industrial Chemicals
Compounds:
dinitrobenzene (p-)
dinitrobenzene (o-)
dinitrobenzene (m-)
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Cadenza HS-C18 (250 x 4.6mm)

Dinitrobenzene

Application #:
TI0283
Type:
Application Note
Categories:
Industrial Chemicals
Compounds:
terephthalonitrile (p-)
phthalonitrile (o-)
isophthalonitrile (m-)
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Cadenza HS-C18 (250 x 4.6mm)

phthalonitriles

Application #:
TI0282
Type:
Application Note
Categories:
Amino Acids and Peptides
Compounds:
NBD-amino acids from rat plasma
Column(s):
Cadenza CD-C18 (250 x 4.6mm)

NBD-amino acids from rat plasma

Application #:
TI0280
Type:
Application Note
Categories:
Botanicals
Compounds:
quercetin
myricetin
kaempferol
chrysin
baicalein
apigenin
acacetin
Column(s):
Cadenza CD-C18 (50 x 2.0mm)

Flavonoids

Application #:
TI0279
Type:
Application Note
Categories:
Botanicals
Compounds:
quercetin
myricetin
kaempferol
chrysin
baicalein
apigenin
acacetin
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Flavonoids

Application #:
TI0278
Type:
Application Note
Categories:
Oligosaccharides
Compounds:
cycloamylose
Column(s):
Cadenza CD-C18 (500 x 4.6mm)

LC-CAD cycloamylose

Application #:
TI0277
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
Morantel Citrate
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza HS-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Morantel Citrate

Application #:
TI0276
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Column Performance
Compounds:
sulfisoxazole
sulfathiazole
sulfaquinoxaline
sulfamonomethoxine
sulfamethoxazole
sulfamethaxine
sulfamerazine
sulfadiazine
Column(s):
Cadenza CD-C18 (20 x 2.0mm)

HT sulfonamides

Application #:
TI0275
Type:
Application Note
Categories:
Industrial Chemicals
Pharmaceuticals & Cosmetics
Compounds:
phenylenediboronic acid
naphthaleneboronic acid
benezeneboronic acid
Column(s):
Cadenza CD-C18 (150 x 3.0mm)

Aromatic boronic acids

Application #:
TI0274
Type:
Application Note
Categories:
Proteins
Compounds:
bovine serum
Column(s):
Intrada WP-RP (250 x 4.6mm)

Bovine serum

Application #:
TI0273
Type:
Application Note
Categories:
Proteins
Compounds:
lactferrin
Column(s):
Intrada WP-RP (250 x 4.6mm)

Lactferrin

Application #:
TI0272
Type:
Application Note
Categories:
Proteins
Compounds:
hemoglobin
Column(s):
Intrada WP-RP (250 x 4.6mm)

Hemoglobin

Application #:
TI0271
Type:
Application Note
Categories:
Proteins
Compounds:
thyroglobulin
Column(s):
Intrada WP-RP (250 x 4.6mm)

Thyroglobulin

Application #:
TI0270
Type:
Application Note
Categories:
Proteins
Compounds:
beta-glucosidase
Column(s):
Intrada WP-RP (250 x 4.6mm)

Beta-Glucosidase

Application #:
TI0269
Type:
Application Note
Categories:
Vitamins
Compounds:
thiamine
riboflavin
pyridoxine
pyridoxal
nicotinic acid
L-ascorbic acid
folic acid
caffeine
Column(s):
Unison UK-C18 (75 x 4.6mm)
Unison US-C18 (150 x 4.6mm)

Waters soluble vitamins

Application #:
TI0268
Type:
Application Note
Categories:
Vitamins
Compounds:
thiamine
pyridoxine
pyridoxamine
pyridoxal
p-aminobenzoic acid
orotic acid
nicotinic acid
nicotinamide
L-ascorbic acid
Column(s):
Unison UK-C18 (75 x 4.6mm)

Water soluble vitamins

Application #:
TI0267
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
ddl (2'3'-dideoxyinosine)
d4T (2'3'-didehydro-3'-deoxythymidine)
AZT (azidothymidine)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

Anti-HIV nucleosides

Application #:
TI0266
Type:
Application Note
Categories:
Column Performance
Industrial Chemicals
Environmental and Agricultural
Compounds:
o-methoxyphenol
m-methoxyphenol
p-methoxyphenol
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CL-C18 (150 x 4.6mm)

Positional Isomers on CL-C18

Application #:
TI0265
Type:
Application Note
Categories:
Column Performance
Environmental and Agricultural
Industrial Chemicals
Compounds:
biphenyl
o-terphenyl
m-terphenyl
triphenylene
p-terphenyl
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)

Separation Properties of Polycyclic Aromatic Compounds on CL-C18

Application #:
TI0264
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
scoporamine hydrobromide
butylscopalamine bromide
atropine sulfate
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

Parasympatholytics

Application #:
TI0263
Type:
Application Note
Categories:
Proteins
Compounds:
interferon alfa-2b
Column(s):
Intrada WP-RP (250 x 4.6mm)

Interferon

Application #:
TI0262
Type:
Application Note
Categories:
Proteins
Compounds:
interferon alfa-2b
Column(s):
Intrada WP-RP (30 x 2.0mm)
Intrada WP-RP (250 x 2.0mm)

Interferon

Application #:
TI0261
Type:
Application Note
Categories:
Industrial Chemicals
Food Chemistry
Pharmaceuticals & Cosmetics
Compounds:
Fe (III)-EDTA
EDTA disodium salt
Cu (II)-EDTA
Column(s):
Unison UK-Phenyl (75 x 4.6mm)

EDTA-Chelates

Application #:
TI0258
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
sulfisoxazole
sulfathiazole
sulfaquinoxaline
sulfamonomethoxine
sulfamethoxazole
sulfamethazine (sulfadimidin)
sulfamerazine
sulfadiazine
Column(s):
Cadenza CD-C18 (30 x 2.0mm)
Cadenza CL-C18 (30 x 2.0mm)
Unison UK-C18 (30 x 2.0mm)

Sulfonamides

Application #:
TI0257
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
uric acid
oxalic acid
L-ascorbic acid
Column(s):
Unison UK-C18 (250 x 4.6mm)

Causative Substances of Urolithiasis

Application #:
TI0256
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
phenylbutazone
oxyphenbutazone
naproxen
meclofenamic acid sodium salt
ketoprofen
flunixin
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

NSAIDs

Application #:
TI0254
Type:
Application Note
Categories:
Column Performance
Steroids
Compounds:
propylbenzoate
deoxycorticosterone acetate
progesterone
butylbenzoate
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)

Separation properties for Neutral Compounds on CL-C18

Application #:
TI0253
Type:
Application Note
Categories:
Column Performance
Compounds:
thiabendazole
o-phenylphenol
imazalil
diphenyl
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)

Electrostatic Interaction on CL-C18

Application #:
TI0252
Type:
Application Note
Categories:
Column Performance
Compounds:
Triphenylene
Phenol
o-Terphenyl
Caffeine
Butylbenzene
Amylbenzene
Column(s):
Cadenza CD-C18 (0 x 0mm)
Cadenza CL-C18 (0 x 0mm)
Unison UK-C18 (0 x 0mm)

Basic interaction of CL-C18, CD-C18, and UK-C18

Application #:
TI0251
Type:
Application Note
Categories:
Column Performance
Compounds:
propylbenzoate
Column(s):
Cadenza CL-C18 (0 x 0mm)

pH durability of CL-C18

Application #:
TI0250
Type:
Application Note
Categories:
Column Performance
Compounds:
Propylparaben
Prednisolone
methyl 3-amino-2-thiophenecarboxylate
Corticosterone
Acetaminophen
6-alpha-methylprednisolone
4-aminobenzophenone
1hydroxy-7-azabenzotriazole
Column(s):
Cadenza CL-C18 (75 x 4.6mm)

Batch to batch reproduciblity of CL-C18

Application #:
TI0249
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Compounds:
chloropheniramine
maleate
allantoin
Column(s):
Unison UK-Silica (250 x 4.6mm)

Ingrediants in eye drops (allantoin and chlorpheniramine, maleate)

Application #:
TI0248
Type:
Application Note
Categories:
LC-MS
Environmental and Agricultural
Compounds:
trichloroacetic acid (TCAA)
monochloroacetic acid (MCAA)
dichloroacetic acid (DCAA)
Column(s):
Unison UK-C18 (75 x 3.0mm)

LC-MS halo acetic acids

Application #:
TI0247
Type:
Application Note
Categories:
Column Performance
Pharmaceuticals & Cosmetics
Biochemistry / Metabolism
Compounds:
procainamide
N-acetylprocainamide
Column(s):
Cadenza CD-C18 (30 x 4.6mm)
Cadenza CL-C18 (30 x 4.6mm)

Separation properties of basic compounds on CL-C18

Application #:
TI0246
Type:
Application Note
Categories:
Column Performance
Biochemistry / Metabolism
Food Chemistry
Pharmaceuticals & Cosmetics
Compounds:
benzoic acid
salicylic acid
theophylline
phenol
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)

Separation properties for acidic compounds on CL-C18

Application #:
TI0245
Type:
Application Note
Categories:
Column Performance
Biochemistry / Metabolism
Compounds:
theophylline
phenol
caffeine
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

Retention behavior of polar compounds

Application #:
TI0244
Type:
Application Note
Categories:
Monoglyceride isomers
Compounds:
2-oleoylglycerol
1-oleoylglycerol (monoolein)
monoelaidin
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-Silica (75 x 4.6mm)

Application #:
TI0243
Type:
Application Note
Categories:
Vanillin and Guaiacol Derivatives
Compounds:
vanillin
guaiacol
ethylvanillin
4-methylguaiacol
4-ethylguaiacol
Column(s):
Cadenza CD-C18 (100 x 4.6mm)
Cadenza CL-C18 (100 x 4.6mm)
Unison UK-C18 (100 x 4.6mm)
Unison UK-C8 (100 x 4.6mm)
Unison UK-Phenyl (100 x 4.6mm)

Application #:
TI0242
Type:
Application Note
Categories:
Carotinoids
Compounds:
astaxanthin
canthaxanthin
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

Application #:
TI0241
Type:
Application Note
Categories:
Dihydrouracil
Compounds:
‚„ihydrouracil
uracil
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Application #:
TI0240
Type:
Application Note
Categories:
Pyromellitic acids
Compounds:
pyromellitic dianhydride
pyromellitic acid
Column(s):
Unison UK-Silica (250 x 4.6mm)

Pyromellitic acids

Application #:
TI0239
Type:
Application Note
Categories:
Halo Acetic Acids
Compounds:
monochloroacetic acid (MCAA)
dichloroacetic acid (DCAA)
trichloroacetic acid (TCAA)
bromochloroacetic acid (BCAA)
monobromoacetic acid (MBAA)
dibromoacetic acid (DBAA)
tribromoacetic acid (TBAA)
Column(s):
Cadenza CL-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Halo acetic acids

Application #:
TI0238
Type:
Application Note
Categories:
Stevia Sweeteners
Compounds:
rebaudioside A
stevioside
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Cadenza CL-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Stevia sweeteners

Application #:
TI0237
Type:
Application Note
Categories:
Fat-Soluble Vitamins
Compounds:
beta-carotene
Vitamin K1
Vitamin A acetate
Vitamin A
Vitamin
Column(s):
Unison UK-Silica (250 x 4.6mm)

Fat soluble vitamins

Application #:
TI0236
Type:
Application Note
Categories:
Agricultural Chemicals (11)
Compounds:
phthalthrin (tetramethrin)
Column(s):
Cadenza CD-C18 (100 x 4.6mm)

Agricultural chemicals

Application #:
TI0235
Type:
Application Note
Categories:
Agricultural Chemicals (10)
Compounds:
iminoctadine (guazatine)
Column(s):
Unison UK-C18 (100 x 4.6mm)

Agricultural chemicals

Application #:
TI0234
Type:
Application Note
Categories:
Agricultural Chemicals (9)
Compounds:
pyrazosulfuron-ethyl
mepronil (basitac)
bromobutide (sumiherb)
ethofenprox
Column(s):
Cadenza CD-C18 (100 x 4.6mm)

Agricultural chemicals

Application #:
TI0233
Type:
Application Note
Categories:
Agricultural Chemicals (8)
Compounds:
benomyl
thiophanate-methyl
azoxystrobin
diethofencarb
fludioxonil
procymidone
mepanipyrim
triflumizole
fluazinam
Column(s):
Cadenza CD-C18 (100 x 4.6mm)

Agricultural chemicals

Application #:
TI0232
Type:
Application Note
Categories:
Agricultural Chemicals (7)
Compounds:
acephate
chromafenozide
fenitrothion
indoxacarb-MP
benfuracarb
emamectin B1a
ethofenprox
silafluofen
Column(s):
Cadenza CD-C18 (100 x 4.6mm)

Agricultural chemicals

Application #:
TI0231
Type:
Application Note
Categories:
Agricultural Chemicals (6)
Compounds:
dinotefuran
nitenpyram
thiamethoxam
imidacloprid
clothianidin
acetamiprid
thiacloprid
Column(s):
Cadenza CD-C18 (100 x 4.6mm)

Agricultural chemicals

Application #:
TI0230
Type:
Application Note
Categories:
Agricultural Chemicals (5)
Compounds:
acephate
dinotefuran
nitenpyram
thiamethoxam
imidacloprid
clothianidin
acetamiprid
thiacloprid
benomyl
pyrazosulfuron-ethyl
thiophanatemethyl
azoxystrobin
diethofencarb
fludioxonil
mepronil (basitac)
chromafenozide
fenitrothion
Column(s):
Cadenza CD-C18 (250 x 4.6mm)

Agricultural chemicals

Application #:
TI0229
Type:
Application Note
Categories:
Synthetic Antimicrobial (Enrofloxacin)
Compounds:
enrofloxacin
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)

Enrofloxacin

Application #:
TI0228
Type:
Application Note
Categories:
Chlorophyll and carotenoid
Compounds:
lutein (xanthophyll)
chlorophyll b
chlorophyll a
beta-carotene
Column(s):
Unison UK-C18 (100 x 4.6mm)

Chlorophyll and carotenoid

Application #:
TI0227
Type:
Application Note
Categories:
ELSD
Compounds:
taurine
thiamine (B1)
pyridoxine (B6)
nicotinamide (B3)
Column(s):
Unison UK-C18 (75 x 4.6mm)

LC-ELSD of taurine and water soluble vitamins

Application #:
TI0226
Type:
Application Note
Categories:
Creatine Metabolites
Compounds:
creatine
creatine phosphate
creatinine
Column(s):
Unison UK-C18 (250 x 4.6mm)

Separation of creatine and related polar metabolites with 100% aqueous mobile phase, using the UK-C18, which excels in the separation of highly polar compounds.

Application #:
TI0225
Type:
Application Note
Categories:
Fat-Soluble Vitamins
Compounds:
alpha-tocopherol
beta-carotene
coenzyme Q10
Column(s):

Comparison of several reverse phase columns in the high-speed separation of fat-soluble vitamins.

Application #:
TI0224
Type:
Application Note
Categories:
Natural Medicine Properties (Shoseiryuto)
Compounds:
paeoniflorin
d-pseudoephedrine
l-ephedrine
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Analysis of components of Shoseiryuto, an herbal medication, including paeoniflorin and ephedrine.

Application #:
TI0223
Type:
Application Note
Categories:
Veterinary Medicine (Synthetic Antimicrobial)
Compounds:
thiamphenicol
levamisol
oxolinic acid
nifurstyreneate monosodium salt
Column(s):
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Four synthetic antibacterial veterinary medications were separated using various columns. Using gradient elution and TFA modifier, the best separation was achieved using a phenyl and C8 phase.

Application #:
TI0222
Type:
Application Note
Categories:
Antibiotic
Compounds:
bacitracin
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of the annular peptide antibiotic bacitracin using various reverse phase columns. Each column produces excellent peaks using gradient elution and TFA modifier.

Application #:
TI0221
Type:
Application Note
Categories:
Antibiotic
Compounds:
colistin
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of the annular peptide antibiotic colistin, which targets the plasma membrane of gram negative bacteria, using various reverse phase columns. All columns achieve excellent separation of colistin isotomers using gradient elution and TFA modifier.

Application #:
TI0220
Type:
Application Note
Categories:
Antibiotic
Compounds:
spiramycin
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of the macrolide antibiotic spiramycin using various reverse phase columns. Each column produces a sharp peak with TFA mobile phase.

Application #:
TI0219
Type:
Application Note
Categories:
Antibiotic
Compounds:
erythromycin
Column(s):
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of the macrolide antibiotic erythromycin using various reverse phase columns. Each column produces a sharp peak at neutral pH.

Application #:
TI0218
Type:
Application Note
Categories:
Veterinary Medicine (Synthetic Antimicrobial)
Compounds:
salinomycin
Column(s):
Unison UK-C18 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of salinomycin, a common antibiotic additive to animal feed. Note the incredible peak produced by the UK Phenyl column.

Application #:
TI0217
Type:
Application Note
Categories:
Veterinary Medicine (Synthetic Antimicrobial)
Compounds:
lasalocid
Column(s):
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of lasalocid, a common antibiotic additive to animal feed, in acid. Each column produces an excellent peak.

Application #:
TI0216
Type:
Application Note
Categories:
Antibiotic
Compounds:
oxytetracycline
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of the antibiotic oxytetracycline, which impedes protein synthesis, using various reverse phase columns. Excellent peaks are produced by all columns in acidic conditions.

Application #:
TI0215
Type:
Application Note
Categories:
Antibiotic
Compounds:
ampicillin
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of penicillin, which impedes synthesis of the bacterial cell wall, using various reverse phase columns. Under acidic conditions all columns produce excellent peaks.

Application #:
TI0214
Type:
Application Note
Categories:
Antibiotic
Compounds:
lincomycin
Column(s):
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Reverse phase separation of the gram negative antibiotic lincomycin in acid produces an excellent peak.

Application #:
TI0213
Type:
Application Note
Categories:
Synthetic Antimicrobial
Compounds:
thiamphenicol
Column(s):
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of the synthetic veterinary antibiotic thiamphenicol, which impedes protein synthesis, under neutral pH. The C18, C8 and Phenyl columns all produce excellent peaks.

Application #:
TI0212
Type:
Application Note
Categories:
Veterinary Medicine (Synthetic Antimicrobial)
Compounds:
levamisol
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of the anthelminthic veterinary drug levamisol. Our reverse phase column with superior end-capping produces excellent peaks under neutral conditions.

Application #:
TI0211
Type:
Application Note
Categories:
Veterinary Medicine (Synthetic Antimicrobial)
Compounds:
oxolinic acid
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of the veterinary antimicrobrial oxolinic acid produces excellent peaks with various reverse phase columns.

Application #:
TI0210
Type:
Application Note
Categories:
Veterinary Medicine (Synthetic Antimicrobial)
Compounds:
nifurstyreneate monosodium salt (NFS)
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Phenyl (75 x 3.0mm)

Analysis of nifurstyreneate monosodium salt, a veterinary antimicrobial nitrofuran used on fish, using various reverse phase columns.

Application #:
TI0209
Type:
Application Note
Categories:
DMSO
Compounds:
dimethyl sulfoxide (DMSO)
Column(s):
Unison UK-Silica (150 x 3.0mm)

The reverse phase separation of the hydrophillic organic solvent dimethyl sulfoxide (DMSO) is difficult. However, our silica columns make a nonaqueous normal phase separation possible.

Application #:
TI0208
Type:
Application Note
Categories:
Anilines
Compounds:
aniline
N-methylaniline
N
N-dimethylaniline
Column(s):
Unison UK-C18 (75 x 4.6mm)

Aniline, methylaniline and dimethylaniline separation with UK-C18.

Application #:
TI0207
Type:
Application Note
Categories:
LC-MS Application for Coenzyme Q10
Compounds:
Coenzyme Q10
Column(s):
Unison UK-C8 (75 x 3.0mm)

500fg of Coenzyme Q10 can be quantitated by utilizing our 3um C8 column and a sensitive LC-MS/MS detector.

Application #:
TI0206
Type:
Application Note
Categories:
LC-MS Application for Calcium Antagonist
Compounds:
diltiazem HCl
Column(s):
Unison UK-C8 (50 x 2.0mm)

25fg of the calcium antagonist diltiazem HCl can be quantitated by utilizing our 3um C8 column and a sensitive LC-MS/MS detector.

Application #:
TI0205
Type:
Application Note
Categories:
LC-MS Application for Antiseptic Substance
Compounds:
chlorhexidine
Column(s):
Unison UK-C8 (50 x 2.0mm)

2.5pg of the disinfectant chlorhexidine are measured with an LC-MS/MS analysis using our 3um C8 column.

Application #:
TI0204
Type:
Application Note
Categories:
ABEE-Converted Oligosaccharides
Compounds:
kojibiosylglucose; kojibiose; nigelose; isomaltose; panose; nigelosylglucose; maltotriose; maltose; glucose; alpha-1
2Gal2; alpha-1
3Gal2; alpha-1
4Gal2; alpha-1
6Gal2
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Application #:
TI0203
Type:
Application Note
Categories:
Utilizing a high-throughput column for enzymatic reaction analysis
Compounds:
p-nitorophenyl-beta-glucoside
p-nitrophenol
Column(s):
Unison UK-C18 (10 x 2.0mm)

Analysis of enzymatic reaction using a high throughput 10 mm column.

Application #:
TI0202
Type:
Application Note
Categories:
Compounds:
alkyl parabens
Column(s):
Unison UK-C18 (10 x 2.0mm)

Our 10 mm long column can be used instead of a conventional 20-35mm column, performing high-speed separation in approximately 20 seconds while reducing column length. Furthermore our 10mm column has an impressive lifetime of over 2000 injections.

Application #:
TI0201
Type:
Application Note
Categories:
Non-Steroidal Anti-Inflammatory Drugs
Compounds:
oxyphenbutazone
ketoprofen
phenylbutazone
naproxen
flunixin
meclofenamic acid sodium salt
Column(s):
Cadenza CD-C18 (150 x 2.0mm)

Analysis of 6 non-steroidal anti-inflammatory drugs under standard LC-MS conditions.

Application #:
TI0200
Type:
Application Note
Categories:
Out of elution order by electrostatic interaction
Compounds:
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

The effect of electrostatic interaction on retention. Hydrophobic interaction, which depends on hydrogen bonding capacity and dipole-dipole interaction, affects the retention time of analysis on different columns.

Application #:
TI0199
Type:
Application Note
Categories:
Adrenergic Amines
Compounds:
octopamine
synephrine
Column(s):
Unison UK-C18 (75 x 2.0mm)

Separation of synephrine, found in the fruit of the Sour Orange Tree, and and octopamine, which operates on the sympathetic nervous system.

Application #:
TI0198
Type:
Application Note
Categories:
Antioxidants
Compounds:
Irganox 1010
Irgafos 168
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison UK-Silica (250 x 4.6mm)

Analysis for antioxidants of plastics in food.

Application #:
TI0197
Type:
Application Note
Categories:
Antiseptics
Compounds:
benzethonium chloride
benzalkonium chloride
Column(s):
Unison UK-C8 (100 x 4.6mm)

Application #:
TI0196
Type:
Application Note
Categories:
Digitalis Glycosides
Compounds:
digoxigenin
digoxin
digitoxigenin
digitoxin
Column(s):
Unison UK-C18 (75 x 4.6mm)

Separation of the cardiac agent digitalis glycoside and its aglycon.

Application #:
TI0195
Type:
Application Note
Categories:
Coenzyme Q10
Compounds:
coenzymeQ10 (ubiquinone-10)
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Silica (150 x 3.0mm)

Analysis of coenzyme Q10, a dietary supplement used for its antioxidant action, with various columns.

Application #:
TI0194
Type:
Application Note
Categories:
Hinokitiol
Compounds:
hinokitiol
benzene
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Analysis of hinokitiol, a compound for whose antibacterial and other physiological properties have been observed.

Application #:
TI0193
Type:
Application Note
Categories:
GDP-Sugars (Sugar-nucleotides)
Compounds:
GDP-mannose
GDP-galactose
Column(s):
Unison UK-C18 (250 x 4.6mm)

Application #:
TI0192
Type:
Application Note
Categories:
UDP-Sugars (Sugar-nucleotides)
Compounds:
UDP-arabinose
UDP-glucose
UDP-xylose
Column(s):
Unison UK-C18 (250 x 4.6mm)

Separation of UDP-sugars, intermediates in sugar chain synthesis, the molecular structure of which differs only in the sugar group.

Application #:
TI0191
Type:
Application Note
Categories:
Betaine
Compounds:
betaine
Column(s):
Unison UK-Silica (250 x 3.0mm)

Reverse phase analysis of the highly polar compound betaine, which is quite difficult to retain in normal phase analysis.

Application #:
TI0190
Type:
Application Note
Categories:
Short-Chain Fatty Acids
Compounds:
n-propionic acid
n-butyric acid
n-valeric acid
n-caproic acid
Column(s):
Unison UK-C18 (50 x 4.6mm)
Unison UK-C8 (50 x 4.6mm)
Unison UK-Phenyl (50 x 4.6mm)

Separation of short chain cable fatty acids without modifying the functional group.

Application #:
TI0189
Type:
Application Note
Categories:
ƒ¿-Solanine and ƒ¿-Chaconine from Potato
Compounds:
alpha-solanine
alpha-chaconine
Column(s):
Cadenza CD-C18 (75 x 2.0mm)

Detection of the alkaloids α- Solanine and α- Chaconine is difficult using UV radiation, but possible with MS analysis.

Application #:
TI0188
Type:
Application Note
Categories:
Theanine in Green Tea
Compounds:
L-theanine
Column(s):
Cadenza CL-C18 (250 x 3.0mm)

Analysis of theanine, a flavorful component of many green teas on the market.

Application #:
TI0187
Type:
Application Note
Categories:
Aromatic Sulfonates
Compounds:
sodium p-toluenesulfonate
sodium benzoate
disodium 2
6-naphthalenedisulfonate
disodium 1
5-naphthalenedisulfonate
sodium xylenesulfonate
sodium 1-naphthalenesulfonate
Column(s):
Cadenza CD-C18 (50 x 4.6mm)

Separation of aromatic sulfonate using a volatile ion pair reagent.

Application #:
TI0186
Type:
Application Note
Categories:
Loading Capacity of Preparative Column
Compounds:
econazole nitrate
miconazole nitrate
Column(s):
Unison US-C18 (250 x 20.0mm)
Unison US-C18 (250 x 4.6mm)

Loading capacity of our versatile 20mm column is demonstrated at the laboratory level.

Application #:
TI0185
Type:
Application Note
Categories:
Saccharin
Compounds:
saccharin
Column(s):
Unison UK-Silica (250 x 3.0mm)

Normal phase analysis of the synthetic sweetener saccharine.

Application #:
TI0184
Type:
Application Note
Categories:
Gamma-Aminobutyric Acid
Compounds:
gamma-aminobutyric acid
Column(s):
Unison UK-C18 (75 x 4.6mm)
Unison UK-Silica (250 x 2.0mm)

Normal phase separation of the neurotransmitter GABA.

Application #:
TI0183
Type:
Application Note
Categories:
Adreno-corticosteroids
Compounds:
aldosterone
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Cadenza CL-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Simultaneous analysis of compounds involved in the synthesis of andrenal corticosteroids.

Application #:
TI0182
Type:
Application Note
Categories:
Antifungal Drugs
Compounds:
econazole nitrate
miconazole nitrate
Column(s):
Unison UK-C18 (150 x 4.6mm)

Separation of the structurally similar antifungal drugs econazole and miconazole.

Application #:
TI0181
Type:
Application Note
Categories:
Xanthine Oxidase Related Compounds
Compounds:
uric acid
hypoxanthine
xanthine
allopurinol
Column(s):
Unison UK-C18 (75 x 4.6mm)

This analysis of xanthine oxydase related compounds includes separation of its substrate, product and inhibitor.

Application #:
TI0180
Type:
Application Note
Categories:
Nucleotides
Compounds:
ATP
ADP
AMP
Column(s):
Unison UK-Silica (250 x 3.0mm)

Analysis of ATP, ADP and AMP in aqueous normal phase using a silica column.

Application #:
TI0179
Type:
Application Note
Categories:
Purine Nucleotide Metabolites (Reversed-Phase)
Compounds:
inosine
hypoxanthine
guanine
xanthine
uric acid
allantoin
allantoic acid
glyoxylic acid
urea
Column(s):
Unison UK-C18 (250 x 3.0mm)

Reverse phase analysis of purine nucleotide metabolites, including uric acid and allantoin.

Application #:
TI0178
Type:
Application Note
Categories:
Purine Nucleotide Metabolites (Normal Phase)
Compounds:
inosine
Column(s):
Unison UK-Silica (250 x 3.0mm)

Normal phase analysis of purine nucleotide metabolites, including uric acid and allantoin, using a silica column.

Application #:
TI0177
Type:
Application Note
Categories:
Catechins in Green Tea
Compounds:
epigallocatechin
catechin
caffeine
epigallocatechin gallate
epicatechin
epicatechin gallate
Column(s):
Cadenza CD-C18 (150 x 2.0mm)

Analysis of catechin enriched green tea using a semi micro column.

Application #:
TI0176
Type:
Application Note
Categories:
5-Fluorouracil (5-FU)
Uracil
Compounds:
uracil
5-fluorouracil
Column(s):
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Reverse phase separation of the highly polar compounds uracil and fluorouracil, which is an anti-cancer drug.

Application #:
TI0175
Type:
Application Note
Categories:
Vitamin
Compounds:
cyanocobalamin
hydroxocobalamin
Column(s):
Unison UK-C18 (150 x 4.6mm)

Gradient elution of cobalamin.

Application #:
TI0174
Type:
Application Note
Categories:
Catecholamines
Compounds:
L-norepinephrine
octopamine
L-epinephrine
dopamine
serotonin
Column(s):
Unison UK-C18 (75 x 4.6mm)

Analysis of the highly polar catecholamines. The ion pair reagent, not included, is separated by the mobile phase.

Application #:
TI0173
Type:
Application Note
Categories:
Acetylsalicylic Acid
Salicylic Acid
Compounds:
acetylsalicylic acid
salicylic acid
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

Application #:
TI0172
Type:
Application Note
Categories:
Acrylamide
Compounds:
methacrylamide
acrylamide
Column(s):
Unison UK-Silica (250 x 2.0mm)

Normal phase separation of acrylamide, the presence of which in common foods has caused health concerns.

Application #:
TI0171
Type:
Application Note
Categories:
Nucleic Acid Bases
Compounds:
thymine
adenine
guanine
cytosine
Column(s):
Unison UK-Silica (250 x 2.0mm)

Separation of highly polar nucleic acid bases in normal phase mode due to high organic solvent density.

Application #:
TI0170
Type:
Application Note
Categories:
L-Carnitine
Compounds:
L-carnitine
Column(s):
Unison UK-C18 (75 x 4.6mm)
Unison UK-Silica (150 x 3.0mm)

The polar compound L-Carnitine, analyzed in both normal and reverse phase modes.

Application #:
TI0169
Type:
Application Note
Categories:
Plant Sterols
Compounds:
brassicastero‚Œ
stigmasterol
campesterol
beta-sitosterol
Column(s):
Cadenza CD-C18 (250 x 4.6mm)

Separation of plant sterols, whose similar structures make separation difficult, using our high resolution column.

Application #:
TI0168
Type:
Application Note
Categories:
Partition Mode / Normal Phase and Reversed-Phase
Compounds:
estrone
ethynylestradiol
alpha-estradiol
beta-estradiol
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Explanation of the difference in elution of estrogens with normal and reverse phase modes.

Application #:
TI0167
Type:
Application Note
Categories:
Separation of high polarity compounds with a 3um silica column
Compounds:
thymidine
adenosine
guanosine
cytidine
Column(s):
Unison UK-Silica (250 x 2.0mm)

Aqueous normal phase separation of nucleosides using a silica column.

Application #:
TI0166
Type:
Application Note
Categories:
High-efficiency separation by a 3um silica particle
Compounds:
alpha-tocopherol
Column(s):
Unison UK-Silica (150 x 4.6mm)

Separation of the difficult to separate group of isomers known as tocopherols in reverse phase mode with a silica column.

Application #:
TI0165
Type:
Application Note
Categories:
Antipyretic Analgesics
Compounds:
acetaminophen
caffeine
salicylamide
promethazine methylenedisalicylate
Column(s):
Unison UK-C18 (75 x 3.0mm)

Analysis of antipyretic analgesics.

Application #:
TI0164
Type:
Application Note
Categories:
Calcium Pantothenate and Ascorbic Acid
Compounds:
calcium pantothenate
ascorbic acid
Column(s):
Cadenza CL-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)