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Technical Information Posters Papers
SEC: IgG, from human serum
SEC: Poly-gamma-glutamic acid
SEC: Myoglobin, from equine skeletal muscle
SEC: Insulin, from bovine pancreas
SEC: Alpha-lactalbumin, from bovine milk
SEC: Alpha-Casein, from bovine milk
SEC: Alpha-chymotrypsinogen A, from bovine pancreas
SEC: Xthantine oxidase (XOD), from Buttermilk
SEC: Beta - Glucosidase
SEC: Alpha - Amylase
LC-MS: Thyroid hormone
LC-MS: Thyroid hormone
LC-MS: Orexin A and B
LC-MS: Kynurenine metabolism pathway
LC-MS: Acidic compound (mobile phase pH)
LC-MS: Insulin (Mobile phase pH)
LC-MS: Amyloid beta-peptide [Human,1-40] (Mobile phase pH)
LC-MS: Amyloid beta-peptide [Human,1-40] (alkali conditions)
Recombinant human serum albumin (rHSA)
LC-MS: retro-dipeptide isomers
LC-MS, Biochemistry/Metabolism, Amino Acids and Peptides
LC-MS: choline metabolites and related compounds
LC-MS: Amino acids in human serum (HClO4)
LC-MS: Amino acids in human serum (SSA)
LC-MS: Synthetic polyamines
LC-MS: Neuroblastoma related compounds
LC-MS: Neurotransmitters (2)
The Scherzo SM-C18 and Unison UK-Amino columns successfully separate the small, polar ascorbic acid related compounds in normal phase mode.
The Scherzo SS-C18 column provides better retention of Shikimic Acid than a standard C18 column.
Cadenza CW-C18, Unison UK-C18, and Unison UK-C8 are all able to resolve the very polar and charged Acetyl CoA and CoA, differing only in an acetyl group.
The Scherzo SM-C18 and Scherzo SS-C18 column resolve very polar carboxylic acids that normally co-elute on a standard C18 column (Unison UK-C18).
The Scherzo SS-C18 column retains Prostaglandins the most, followed by Scherzo SM-C18 and Scherzo SW-C18.
Under neutral pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.
Under acidic pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.
On the Unison UK-C18 column, tetrahydrofuran gives better resolution of Prostaglandins than acetonitrile and acetone.
Acetonitrile and methanol have opposite retention properties in the separation of prostaglandins on the Unison UK-C18 column.
The Scherzo SM-C18 column can separate these two molecules, which would normally coelute on a standard C18 column.
The Scherzo SM-C18 and the Scherzo SW-C18 show similar retention of these small molecules as the standard Unison UK-C18. However, the Scherzo SS-C18 shows the best resolution between these three compounds.
The Scherzo SS-C18 column retains and separates glutamic acid and GABA better than a conventional C18 column
Small, polar molecules like neurotransmitters are difficult to retain on a conventional C18 column like Unison UK-C18. Scherzo SS-C18 can retain these compounds much better than a standard Unison UK-C18 column, as well as resolve the peaks better. Conditions compatible with LC-MS
The highly charged phosphate groups make separation of AMP, ADP, and ATP difficult on a conventional C18 column. Scherzo SW-C18 is able to elute these compounds effectively, in both ammonium acetate and ammonium phosphate buffers.
Serotonin related compounds
Exploring different C18 stationary phases for metabolites profiling
Simulatneous HPLC/LT-ELSD Analysis of Organic and Phenolic Acids
Simulatneous HPLC/LT-ELSD Analysis of Water Soluble and Fat Soluble Vitamins
Scherzo is able to separate mandelic acid and dopamine, which coelute on a conventional C18 column (Unison UK-C18). This is possible because the ionic ligands recognize the small structural differences between the two compounds.
The effect of gradient slope and column length for thyroglobulin separation
LC-MS(MRM) Application for Monosaccharides
Cyclic mononucleotides (cAMP, cGMP)
Levulinic Acid, Lactic Acid and methyl Esters
Coenzymes (Nicotinamide adenine dinucleotides)
Adenosine Phosphates (AMP, ADP, ATP)
Xanthines and Metabolites
Xanthines and Metabolites in Bovine Serum
Xanthines and Metabolites
5-Fluorouracil and Related Compounds
LC-MS/MS Application - Steroid Hormones and Metabolites
The Unison UK-Amino column shows excellent recognition of both the oxidized and reduced forms of glutathione.
Nicotine derivatives and metabolites can be separated by both reversed phase and normal phase chromatography. Reversed phase gives better run time.
Cadenza HS-C18 successfully separates out small molecules like Estradiol and metabolites from bovine serum. The sample chromatogram looks almost identical to the standard chromatogram.
Many Cadenza and Unison columns achieve excellent resolution with beta-Estradiol 3,17-disulfate and 17beta-estradiol despite their similar structures.
Unison UK-C18 successfully separates methylated arginines of similar structure. Compounds are pre-column derivatized to enable fluroescence detection.
Unison UK-C18 successfully separates nitroNE, nitroE, and nitroDA. Detection is via post-column derivatization.
Unison UK-Amino column separates catecholamines of similar structure using normal phase/anion exchange mode.
Separation properties of basic compounds on CL-C18
Separation properties for acidic compounds on CL-C18
Separation of creatine and related polar metabolites with 100% aqueous mobile phase, using the UK-C18, which excels in the separation of highly polar compounds.
Analysis of coenzyme Q10, a dietary supplement used for its antioxidant action, with various columns.
Separation of UDP-sugars, intermediates in sugar chain synthesis, the molecular structure of which differs only in the sugar group.
Separation of short chain cable fatty acids without modifying the functional group.
This analysis of xanthine oxydase related compounds includes separation of its substrate, product and inhibitor.
Reverse phase analysis of purine nucleotide metabolites, including uric acid and allantoin.
Analysis of the highly polar catecholamines. The ion pair reagent, not included, is separated by the mobile phase.
Caproic acid and its methylvaleric acid isomers are separated with high resolution using our 500mm long column.
Simultaneous analysis of Citric Acid Cycle compounds using our phenyl column.
Simultaneous analysis of Ornithine Cycle comounds using our UK-C18, which has affinity for highly polar compounds.
High-speed separations with Unison UK-C8 150mm column compared with a 5um 250mm column popular on the market.
Separation of hippuric acid, a metabolite of toluene and xylene. Cadenza can recognize positional isomers of methyl hippuric acid as well.
The world's first specialty HPLC column for the analysis of amino acids without derivatization using LC-MS
Amino acids and metabolites/derivatives analysis without derivatization using a novel mixed-mode column
An Evaluation of a Multi-Mode Stationary Phase for HPLC Separations Using a Multi-Component Test Mix
Advantage of a New Generation of Evaporative Light-Scattering Detectors: Universality, Higher Sensitivities and Cost-Effectiveness for Multi-Element Analyses in Liquid Chromatography - An Application Review
High-throughput non-targeted metabolic profiling with hybrid stationary phase LC column coupled to Q-TOF-MS in cancer research
A relative quantitative positive/negative ion switching method for untargeted lipidomics via high resolution LC-MS/MS from any biological source