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Technical Information   Posters   Papers

Application #:
TI0943
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
Argininosuccinic Acid
Methylnicotinamide
Column(s):
Intrada Amino Acid (150 x 3.0mm)

LC-TOFMS: Urinary compounds

Application #:
TI0942
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
Coenzyme A (CoA)
Acetyl Coenzyme A (Acetyl-CoA)
Acetoacetyl Coenzyme A (Acetoacetyl-CoA)
Flavin Adenine Dinucleatide (FAD)
UDP-N-Acetylglucosamine
Column(s):
Intrada Amino Acid (150 x 3.0mm)

LC-TOFMS: Coenzymes

Application #:
TI0941
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
5-Methyltetrahydrofolate
5
10-Methenyltetrahydrofolate
Column(s):
Intrada Amino Acid (150 x 3.0mm)

LC-TOFMS: Methylfolate

Application #:
TI0940
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
Glutathione reduced (GSH)
Glutathione oxidized (GSSG)
Column(s):
Intrada Amino Acid (150 x 3.0mm)

LC-TOFMS: Glutathione

Application #:
TI0939
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
5-Methylcytosine (5mC)
Cytidine Monophosphate (CMP)
Cytidine Diphosphate (CDP)
Cytidine Triphosphate (CTP)
Column(s):
Intrada Amino Acid (150 x 3.0mm)

LC-TOFMS: Cytosine compounds

Application #:
TI0938
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
Creatine
Creatinine
Phosphocreatine
Column(s):
Intrada Amino Acid (150 x 3.0mm)

LC-TOFMS: Creatine compounds

Application #:
TI0937
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
Choline
Acetylcholine
Phosphocholine
Glycerophosphocholine
CDP-Choline
Column(s):
Intrada Amino Acid (150 x 3.0mm)

LC-TOFMS: Choline compounds

Application #:
TI0919
Type:
Application Note
Categories:
Proteins
Biochemistry/Metabolism
Compounds:
IgG
Column(s):
(250 x 4.6mm)

SEC: IgG, from human serum

Application #:
TI0901
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Food Chemistry
Pharmaceuticals and Cosmetics
Compounds:
Poly-gamma-glutamic acid
Column(s):
(250 x 4.6mm)

SEC: Poly-gamma-glutamic acid

Application #:
TI0900
Type:
Application Note
Categories:
Biochemistry/Metabolism
Polysaccharides
Compounds:
Proteoglycan
Column(s):
(250 x 4.6mm)

SEC: Proteoglycan

Application #:
TI0899
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Compounds:
Ovalbumin
Column(s):
(250 x 4.6mm)

SEC: Ovalbumin

Application #:
TI0898
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Compounds:
Myoglobin
Column(s):
(250 x 4.6mm)

SEC: Myoglobin, from equine skeletal muscle

Application #:
TI0897
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Compounds:
Insulin
Column(s):
(250 x 4.6mm)

SEC: Insulin, from bovine pancreas

Application #:
TI0895
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Food Chemistry
Compounds:
Alpha-lactalbumin
Column(s):
(250 x 4.6mm)

SEC: Alpha-lactalbumin, from bovine milk

Application #:
TI0894
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Food Chemistry
Compounds:
Alpha-Casein
Column(s):
(250 x 4.6mm)

SEC: Alpha-Casein, from bovine milk

Application #:
TI0893
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Food Chemistry
Compounds:
alpha-chymotrypsinogen A
Column(s):
(250 x 4.6mm)

SEC: Alpha-chymotrypsinogen A, from bovine pancreas

Application #:
TI0892
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Food Chemistry
Compounds:
Xthantine Oxidase
Column(s):
(250 x 4.6mm)

SEC: Xthantine oxidase (XOD), from Buttermilk

Application #:
TI0891
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Food Chemistry
Compounds:
beta-glucosidase
Column(s):
(250 x 4.6mm)

SEC: Beta - Glucosidase

Application #:
TI0889
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Food Chemistry
Compounds:
alpha-amylase
Column(s):
(250 x 4.6mm)

SEC: Alpha - Amylase

Application #:
TI0883
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Biochemistry/Metabolism
Compounds:
Histamine
Column(s):
Scherzo SM-C18 (150 x 4.6mm)

Histamine

Application #:
TI0854
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Column Performance
Compounds:
triiodothyronine (T3)(L-3
3'
5-triiodothyronine)
thyroxine (T4)(L-3
5
3'
5'-tetraiodothyronine)
Column(s):
Scherzo SM-C18 (50 x 3.0mm)
Cadenza CW-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)
Unison UK-C8 (50 x 3.0mm)
Unison UK-Phenyl (50 x 3.0mm)

LC-MS: Thyroid hormone

Application #:
TI0853
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
triiodothyronine (T3) (L-3
3'
5-triiodothyronine)
thyroxine (T4) (L-3
5
3'
5'-tetraiodothyronine)
Column(s):
Intrada Amino Acid (50 x 3.0mm)
Scherzo SM-C18 (50 x 3.0mm)

LC-MS: Thyroid hormone

Application #:
TI0849
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
orexin A
orexin B
Column(s):
Scherzo SW-C18 (50 x 3.0mm)
Unison UK-Phenyl (100 x 3.0mm)

LC-MS: Orexin A and B

Application #:
TI0848
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
orexin A
orexin B
Column(s):
Intrada WP-RP (100 x 3.0mm)
Cadenza CD-C18 (100 x 3.0mm)
Cadenza CW-C18 (100 x 3.0mm)
Cadenza CL-C18 (100 x 3.0mm)
Unison UK-C18 (100 x 3.0mm)
Unison UK-C8 (100 x 3.0mm)
Unison UK-Phenyl (100 x 3.0mm)

LC-MS: Orexin A and B

Application #:
TI0847
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
orexin A
orexin B
Column(s):
Scherzo SW-C18 (50 x 3.0mm)

LC-MS: Orexin A and B

Application #:
TI0846
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
quinolinic acid
kynurenic acid
picolinic acid
3-hydroxyanthranilic acid
L-kynurenine
L-tryptophan
Column(s):
Intrada Amino Acid (100 x 3.0mm)

LC-MS: Kynurenine metabolism pathway

Application #:
TI0837
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
S-(5'-adenosyl)-L-homocysteine (SAH)
S-(5'-adenosyl)-L-methionine (SAM)
Column(s):
Scherzo SW-C18 (50 x 3.0mm)

LC-MS: SAH and SAM

Application #:
TI0834
Type:
Application Note
Categories:
Column Performance
LC-MS
Biochemistry/Metabolism
Compounds:
cholic acid
Column(s):

LC-MS: Acidic compound (mobile phase pH)

Application #:
TI0831
Type:
Application Note
Categories:
Column Performance
LC-MS
Amino Acids and Peptides
Biochemistry/Metabolism
Compounds:
Insulin
Column(s):

LC-MS: Insulin (Mobile phase pH)

Application #:
TI0830
Type:
Application Note
Categories:
Column Performance
LC-MS
Amino Acids and Peptides
Biochemistry/Metabolism
Compounds:
Amyloid beta-peptide [Human
1-40]
Column(s):

LC-MS: Amyloid beta-peptide [Human,1-40] (Mobile phase pH)

Application #:
TI0829
Type:
Application Note
Categories:
Column Performance
LC-MS
Amino Acids and Peptides
Biochemistry/Metabolism
Compounds:
Amyloid beta-peptide [Human
1-40]
Column(s):

LC-MS: Amyloid beta-peptide [Human,1-40] (alkali conditions)

Application #:
TI0822
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Lipid A
Column(s):
Unison UK-Silica (150 x 4.6mm)

Lipid A (Salmonella minnesota Re595)

Application #:
TI0821
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Column Performance
Compounds:
Recombinant human serum albumin (rHSA)
Column(s):
Intrada WP-RP (250 x 4.6mm)
Cadenza CW-C18 (250 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

Recombinant human serum albumin (rHSA)

Application #:
TI0820
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Compounds:
human serum albumin (HSA)
Column(s):
Presto FF-C18 (250 x 4.6mm)

Recombinant and biosynthetic human serum albumin (HSA)

Application #:
TI0819
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Compounds:
Recombinant human serum albumin (rHSA)
Column(s):
Presto FF-C18 (250 x 4.6mm)

Recombinant human serum albumin (rHSA)

Application #:
TI0818
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
L-glutamic acid
L-glutamyl-L-glutamic acid (Glu-Glu)
L-alanine
L-glutamine (Ala-Gln)
L-glutaminyl-L-alanine (Gln-Ala)
L-leucine
L-histidine
L-leucyl-L-histidine (Leu-His)
L-histidyl-L-leucine (His-Leu)
Column(s):
Intrada Amino Acid (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)

LC-MS: retro-dipeptide isomers

Application #:
TI0817
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
homovanillic acid (HVA)
3
4-dihydroxyphenyl acetic acid (DOPAC)
L-tyrosine
3
4-dihydroxy-L-phenylalanine (L-DOPA)
3-methoxytyramine
dopamine
Column(s):
Intrada Amino Acid (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)

LC-MS, Biochemistry/Metabolism, Amino Acids and Peptides

Application #:
TI0816
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
1
2-dimyristoyl-sn-glycero-3-phosphocholine
1
2-dimyristoyl-sn-glycero-3-phosphoethanolamine
acetylcholine chloride
trimethylglycine (betaine)
N
N-dimethylglycine
S-(5'-adenosyl)-L-homocysteine (SAH)
L-methionine
phosphocholine
cytidine 5'-diphosphocholine
choline chloride
S-(5'-adenosyl)-L-methionine (SAM)
Column(s):
Intrada Amino Acid (100 x 3.0mm)

LC-MS: choline metabolites and related compounds

Application #:
TI0815
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
L-isoproterenol
Column(s):
Intrada Amino Acid (100 x 3.0mm)
Scherzo SS-C18 (100 x 3.0mm)

LC-MS: isoproterenol

Application #:
TI0814
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
L-methionine
L-carnitine
L-lysine
Column(s):
Intrada Amino Acid (50 x 3.0mm)
Scherzo SS-C18 (50 x 3.0mm)

LC-MS: carnitine related compounds

Application #:
TI0813
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
Phe
Trp
Ile
Leu
Met
Tyr
Pro
Val
Ala
Thr
Glu
Asp
Gly
Ser
Gln
Asn
(Cys)2
His
Lys
Arg
Column(s):
Intrada Amino Acid (100 x 3.0mm)

LC-MS: Amino acids in human serum (HClO4)

Application #:
TI0812
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
Phe
Trp
Ile
Leu
Met
Tyr
Pro
Val
Ala
Thr
Glu
Asp
Gly
Ser
Gln
Asn
(Cys)2
His
Lys
Arg
Column(s):
Intrada Amino Acid (100 x 3.0mm)

LC-MS: Amino acids in human serum (SSA)

Application #:
TI0811
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
L-cystine
L-homocystine
L-glutamyl-L-glutamic acid (Glu-Glu)
1-glycyl-L-proline (Gly-Pro)
L-lysinoalanine
L-alanyl-L-glutamine (Ala-Gln)
L-anserine
L-carnosine
L-aspartyl-L-phenylalanine methyl ester (aspartame)
L-histidyl-L-leucine (His-Leu)
N-(3
3-dimethylbutyl)-L-aspartyl-L-phenylalanine methyl ester (neotame)
Column(s):
Scherzo SS-C18 (150 x 3.0mm)

LC-MS: dipeptides

Application #:
TI0807
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
L-epinephrine (L-adrenaline)
L-metanephrine
Column(s):
Scherzo SS-C18 (30 x 2.0mm)

LC-MS: Epinephrine and metanephrine

Application #:
TI0806
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Synthetic Polymers
Compounds:
ethylenediamine
hexamethylenediamine
diethylenetriamine
triethylenetetramine
tetraethylenepentamine
bis(6-aminohexyl)amine
Column(s):
Scherzo SM-C18 (50 x 3.0mm)

LC-MS: Synthetic polyamines

Application #:
TI0805
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
1
3-propanediamine
putrescine
cadaverine
norspermidine
spermidine
spermine
Column(s):
Scherzo SM-C18 (50 x 3.0mm)

LC-MS: Biogenic polyamines

Application #:
TI0803
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Column Performance
Compounds:
acetylcholine
d4-acetylcholine
Column(s):
Scherzo SS-C18 (50 x 2.0mm)

LC-MS/MS: Acetylcholine and d4-acetylcholine

Application #:
TI0802
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Compounds:
L-vanillylmandelic acid (VMA)
L-mandelic acid (MA)
homovanillic acid (HVA)
Column(s):
Unison UK-C18 (50 x 3.0mm)
Unison UK-Phenyl (50 x 3.0mm)

LC-MS: Neuroblastoma related compounds

Application #:
TI0801
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
5-hydroxyindole-3-acetic acid (5-HIAA)
L-glutamic acid (glu)
L-phenylalanine (phe)
5-hydroxy-L-tryptophan
L-tyrosine (tyr)
L-tryptophan (trp)
3
4-dihydroxy-L-phenylalanine (L-DOPA)
4-aminobutyric acid (GABA)
acetylcholine chloride
L-adrenaline (epinephrine)
L-noradrenaline (norepinephrine)
dopamine
serotonin (5-ht)
Column(s):
Intrada Amino Acid (100 x 3.0mm)

LC-MS: Neurotransmitters (2)

Application #:
TI0733
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
adenosine
hypoxanthine
inosine
Column(s):
Unison UK-Amino (75 x 3.0mm)

The Unison UK-Amino column successfully separates adenosine and inosine, which are very similar in structure.

Application #:
TI0732
Type:
Application Note
Categories:
Biochemistry/Metabolism
Vitamins
Industrial Chemical
Food Chemistry
Compounds:
"isoascorbic acid (erythorbic acid)
L-ascorbic acid
L-ascorbic acid 2-glucoside"
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-Amino (75 x 3.0mm)

The Scherzo SM-C18 and Unison UK-Amino columns successfully separate the small, polar ascorbic acid related compounds in normal phase mode.

Application #:
TI0731
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
shikimic acid
L-tyrosine
L-phenylalanine
L-tryptophan
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

The Scherzo SS-C18 column provides better retention of Shikimic Acid than a standard C18 column.

Application #:
TI0727
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Coenzyme A (CoA)
acetyl coenzyme (Acetyl-CoA)
Column(s):
Cadenza CW-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)

Cadenza CW-C18, Unison UK-C18, and Unison UK-C8 are all able to resolve the very polar and charged Acetyl CoA and CoA, differing only in an acetyl group.

Application #:
TI0720
Type:
Application Note
Categories:
Biochemistry/Metabolism
Botanicals
Compounds:
Shikimic acid
malic acid
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

The Scherzo SM-C18 and Scherzo SS-C18 column resolve very polar carboxylic acids that normally co-elute on a standard C18 column (Unison UK-C18).

Application #:
TI0719
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)

The Scherzo SS-C18 column retains Prostaglandins the most, followed by Scherzo SM-C18 and Scherzo SW-C18.

Application #:
TI0718
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)
Unison UK-Phenyl (150 x 3.0mm)

Under neutral pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

Application #:
TI0717
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza CW-C18 (150 x 3.0mm)
Cadenza CL-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)
Unison UK-C8 (150 x 3.0mm)
Unison UK-Phenyl (150 x 3.0mm)

Under acidic pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

Application #:
TI0716
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Unison UK-C18 (150 x 3.0mm)

On the Unison UK-C18 column, tetrahydrofuran gives better resolution of Prostaglandins than acetonitrile and acetone.

Application #:
TI0715
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Prostaglandin F2
Prostaglandin E2
Column(s):
Unison UK-C18 (150 x 3.0mm)

Acetonitrile and methanol have opposite retention properties in the separation of prostaglandins on the Unison UK-C18 column.

Application #:
TI0710
Type:
Application Note
Categories:
Biochemistry and Metabolism
Compounds:
CTP
UTP
TTP
dCTP
GTP
ATP
dGTP
dTTP
dATP
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

The Scherzo SM-C18 column successfully resolves nucleotide triphosphates of similar structure.

Application #:
TI0704
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Spermine and spermidine
Column(s):
Scherzo SM-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)

The Scherzo SM-C18 column can separate these two molecules, which would normally coelute on a standard C18 column.

Application #:
TI0701
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
Botanicals
Biochemistry/Metabolism
Compounds:
Allantoin
allantoic acid and uric acid
Column(s):
Scherzo SW-C18 (250 x 3.0mm)
Scherzo SM-C18 (250 x 3.0mm)
Scherzo SS-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

The Scherzo SM-C18 and the Scherzo SW-C18 show similar retention of these small molecules as the standard Unison UK-C18. However, the Scherzo SS-C18 shows the best resolution between these three compounds.

Application #:
TI0698
Type:
Application Note
Categories:
Biochemistry/Metabolism
Amino Acids and Peptides
Compounds:
L-glutamic acid
gamma-aminobutyric acid (GABA)
Column(s):
Scherzo SS-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

The Scherzo SS-C18 column retains and separates glutamic acid and GABA better than a conventional C18 column

Application #:
TI0694
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
"L-glutamic acid
gammaaminobutyric acid (GABA)
3
4-dihydroxyL-phenylalanine (L-DOPA)
5-hydroxyindole- 3-acetic acid (5-HIAA)
L-tyrosine
L-noradrenaline (L-norepinephrine)
acetylcholine chloride
L-adrenaline (L-epinephrine)
5-hydroxyL-tryptophan
L-phenylalanine
dopamine
L-tryptophan
serotonin"
Column(s):
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Small, polar molecules like neurotransmitters are difficult to retain on a conventional C18 column like Unison UK-C18. Scherzo SS-C18 can retain these compounds much better than a standard Unison UK-C18 column, as well as resolve the peaks better. Conditions compatible with LC-MS

Application #:
TI0691
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
AMP
ADP
ATP
Column(s):
Scherzo SW-C18 (150 x 3.0mm)

The highly charged phosphate groups make separation of AMP, ADP, and ATP difficult on a conventional C18 column. Scherzo SW-C18 is able to elute these compounds effectively, in both ammonium acetate and ammonium phosphate buffers.

Application #:
TI0684
Type:
Application Note
Categories:
Biochemistry/Metabolism
Botanicals
Proteins
Compounds:
cytochrome c (horse heart
12.4 kDa)
Column(s):
Presto FF-C18 (150 x 3.0mm)

Cytochrome c

Application #:
TI0683
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
"5-hydroxy-L-tryptophan
serotonin
L-tryptophan
5-hydroxyindole -3-acetic acid (5-HIAA)"
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)

Serotonin related compounds

Application #:
TI0682
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
"L-histidine
N-methyl-4- imidazoleacetic acid
4-imidazoleacetic acid
N-methylhistamine
histamine"
Column(s):
Scherzo SS-C18 (150 x 3.0mm)

Histamine related compounds

Application #:
TI0681
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
L-glutamic acid (Glu)
4-aminobutyric acid (GABA)
L-DOPA
acetylcholine chloride
L-adrenaline
L-noradrenaline
dopamine
Column(s):
Scherzo SS-C18 (150 x 3.0mm)
Scherzo SS-C18 (250 x 3.0mm)

Neurotransmitters

Application #:
TI0671
Type:
Application Note
Categories:
Biochemistry and Metabolism
Amino Acids and Peptides
Compounds:
creatine phosphate
betaine
L-carnitine
creatine
L-theanine
L-orthinine
Column(s):
Scherzo SS-C18 (150 x 3.0mm)

Zwitterionic compounds

Application #:
TI0670
Type:
Application Note
Categories:
Biochemistry and Metabolism
Amino Acids and Peptides
Compounds:
creatine phosphate
betaine
L-carnitine
creatine
L-theanine
Column(s):
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Zwitterionic compounds

Application #:
TI0669
Type:
Application Note
Categories:
Biochemistry/Metabolism
Pharmaceuticals and Cosmetics
Compounds:
gamma-aminobutyric acid (GABA)
D
L-noradrenaline ((D
L-norepinephrine)
D
L-adrenaline (D
L-epinephrine)
3
4-dihydroxy-L-phenylalanine (L-DOPA)
dopamine
Column(s):
Scherzo SW-C18 (150 x 3.0mm)
Scherzo SM-C18 (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)

Catecholamines

Application #:
TI0652
Type:
Application Note
Categories:
Column Performance
Biochemistry/Metabolism
LC-MS
Compounds:
alanine
N
N-dimethylglycine
serine
fumaric acid
succinic acid
cysteine
oxaloacetic acid
Gly-Gly
malic acid
alpha-ketoglutarate
citric acid
2-methylhippuric acid
gamma-D-glutamylglycine
salbutomal
AMP
ribo-avin
Phe-gly-phe-gly
s-(p-nitrobenzyl) glutathione
s-(p-azidophenacyl)glutathione
taurocholic acid
O-beta-D-glucuronosyl-naphthol AS-BI
Leucine enkephalin
Arg-Pro-Pro-Gly-Phe
Reserpine
GSSG
Arg-Lys-Asp-Val-Tyr
Coenzyme A
Acetyl CoA
Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr (peptide T)
bradykinin fragment 1-8
Tyr-tyr-tyr-tyr-tyr-tyr
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

Exploring different C18 stationary phases for metabolites profiling

Application #:
TI0649
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
Quinic acid
Malic acid
Tartaric acid
Succinic acid
citric acid
gallic acid
lactic acid
protocatechuic acid
4HPAC
chlorogenic acid
syringic acid
gentisic acid
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

Simulatneous HPLC/LT-ELSD Analysis of Organic and Phenolic Acids

Application #:
TI0648
Type:
Application Note
Categories:
Biochemistry/Metabolism
Vitamins
Compounds:
B1:thiamine
B8:myo-Inositol
B6:Pyridoxine
C:Ascorbic acid
B5:Panthothenic acid
B9: Folic acid
B12: Cyanocobalamin
B2:Riboflavin
B7: Biotin
A:Retinol
K2:Menaquinone
D2:Ergocalciferol
D3:Cholecalciferol
E:a-Tocopherol
K1: Phylloquinone
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

Simulatneous HPLC/LT-ELSD Analysis of Water Soluble and Fat Soluble Vitamins

Application #:
TI0642
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
vanillylmandelic acid
mandelic acid
homovanillic acid
dopamine
Column(s):
Scherzo SM-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Scherzo is able to separate mandelic acid and dopamine, which coelute on a conventional C18 column (Unison UK-C18). This is possible because the ionic ligands recognize the small structural differences between the two compounds.

Application #:
TI0641
Type:
Application Note
Categories:
Biochemistry/Metabolism
Compounds:
vanillylmandelic acid
mandelic acid
homovanillic acid
dopamine
Column(s):
Scherzo SM-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Catecholamine metabolites

Application #:
TI0634
Type:
Application Note
Categories:
Column Performance
Proteins
Biochemistry/Metabolism
Compounds:
thyroglobulin( porcin thyroid)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for thyroglobulin separation

Application #:
TI0630
Type:
Application Note
Categories:
Column Performance
Proteins
Biochemistry/Metabolism
Compounds:
insulin
Column(s):
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope for insulin separation

Application #:
TI0629
Type:
Application Note
Categories:
Column Performance
Biochemistry/Metabolism
Amino acids and peptides
Compounds:
angiotensin II
Column(s):
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope for angiotensin II serparation

Application #:
TI0612
Type:
Application Note
Categories:
Oligosaccharides
Biochemistry / Metabolism
Botanicals
Compounds:
Unsaturated Chondro-Disaccharides
Column(s):
Unison UK-Amino (250 x 3.0mm)

Unsaturated Chondro-Disaccharides

Application #:
TI0608
Type:
Application Note
Categories:
Saccharides
LC-MS
Biochemistry / Metabolism
Compounds:
fucose
xylose
N-acetylgalactosamine
N-acetylglucosamine
mannose
galactose
glucose
N-acetylneuraminic acid
N-glycolylneuraminic acid
Column(s):
Unison UK-Amino (250 x 2.0mm)

LC-MS(MRM) Application for Monosaccharides

Application #:
TI0607
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
sodium gluconate
acetic acid
lactic acid
glyoxylic acid
formic acid
Column(s):
Unison UK-Amino (250 x 4.6mm)

Organic acids (Anion exchange mode)

Application #:
TI0601
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
N-methylthiourea
urea
3-hydroxyphenylurea
hydroxyurea
Column(s):
Unison UK-Amino (250 x 4.6mm)

Urea and related compounds

Application #:
TI0599
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
NAD
NADP
NADH
NADPH
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Coenzymes (Nicotinamide adenine dinucleotides)

Application #:
TI0598
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
NAD
NADP
NADH
NADPH
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Coenzymes (Nicotinamide adenine dinucleotides)

Application #:
TI0588
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
adenosine 5'- monophosphate disodium (AMP) guanosine 5'- monophosphate disodium (GMP)
adenosine 3'
5'-cyclic monophosphate sodium (cAMP)
guanosine 3'
5'-cyclic monophosphate sodium (cGMP)
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Cyclic mononucleotides (cAMP, cGMP)

Application #:
TI0548
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
succinic acid
malic acid
fumaric acid
malonic acid
citric acid
tartaric acid
maleic acid
Column(s):
Scherzo SM-C18 (250 x 3.0mm)
Unison UK-C18 (250 x 3.0mm)

Organic acids (2)

Application #:
TI0542
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
succinic acid
lactic acid
sodium gluconate
malic acid
glyoxylic acid
fumaric acid
maloinic acid
citric acid
tartaric acid
maleic acid
Column(s):
Unison UK-Amino (250 x 3.0mm)

Organic Acids

Application #:
TI0539
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
adenine
guanine
hypoxanthine
xanthine
Column(s):
Scherzo SM-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Purine bases

Application #:
TI0536
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Biochemistry / Metabolism
Compounds:
N-methylhydantoin
methimazole
N-methylthiourea
2-mercaptoimidazole
Column(s):
Unison UK-Amino (250 x 3.0mm)

Methimazole and potential metabolites

Application #:
TI0530
Type:
Application Note
Categories:
Biochemistry / Metabolism
Industrial Chemical
Compounds:
methyl lactate
lactic acid
levulinic acid
methyl levulinate
Column(s):
Scherzo SM-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Levulinic Acid, Lactic Acid and methyl Esters

Application #:
TI0529
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
ADP
AMP
ATP
Column(s):
Scherzo SM-C18 (30 x 3.0mm)
Unison UK-C18 (30 x 3.0mm)

Adenosine Phosphates

Application #:
TI0524
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
L-carnosine
L-anserine nitrate
Inosine 5' monophosphate dipotassium
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Anserine and Related Compounds

Application #:
TI0522
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
CMP
UMP
dCMP
dUMP
IMP
GMP
dTMP
AMP
dGMP
dAMP
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Mononucleotides

Application #:
TI0521
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
glutathione reduced (GSH)
glutathione oxidized (GSSG)
Column(s):
Scherzo SM-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)

Glutathiones

Application #:
TI0520
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Biochemistry / Metabolism
Compounds:
sodium chloride
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

Sodium Chloride

Application #:
TI0519
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
DL-mevalonolactone
DL-mevalonic acid
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Mevalonolactone and Mevalonic Acid

Application #:
TI0518
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
sodium gluconate
glyoxylic acid
lactic acid
malic acid
succinic acid
malonic acid
citric acid
fumaric acid
maleic acid
Column(s):
Scherzo SM-C18 (150 x 3.0mm)
Unison UK-C18 (150 x 3.0mm)

Organic Acids

Application #:
TI0517
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
beta-estradiol 17-(beta-glucuronide)
17beta-estradiol
beta-estradiol 3
17 disulfate
Column(s):
Scherzo SM-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)

Estradiol and Metabolites

Application #:
TI0512
Type:
Application Note
Categories:
Biochemistry/Metabolism
LC-MS
Compounds:
Sphingomyelin from sheep erythrocytes
Column(s):
Cadenza CD-C18 (150 x 2.0mm)

Identification of sphingomyelin molecular species with LC-MS/MS

Application #:
TI0493
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
urine
Column(s):
Cadenza HS-C18 (100 x 2.0mm)

LC-Q-TOF-MS application for high-throughput non-targeted metabolomics with a hybrid ODS column

Application #:
TI0491
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
Sphinganine-1-Phosphate (Dihydrosphingosine-1-phosphate)
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Sphinganine-1-PO4)

Application #:
TI0490
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
Sphingosine-1-Phosphate
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Sphingosine-1-PO4)

Application #:
TI0489
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
Sphingosine
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Sphingosine)

Application #:
TI0488
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
Sphinganine (Dihydrosphingosine)
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Sphinganine)

Application #:
TI0487
Type:
Application Note
Categories:
LC-MS
Biochemistry / Metabolism
Compounds:
C16 Ceramide
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

LC-MS/MS Application for Sphingolipids (Ceramide)

Application #:
TI0477
Type:
Application Note
Categories:
Biochemicals and Metabolites
Compounds:
Phosphatidylinositol
Phosphatidylcholine
Phosphatidylethanolamine
Phosphatidylglycerol
Sphingomyelin
Column(s):
Cadenza CW-C18 (100 x 2.0mm)

LC-MS Application for Phospholipids

Application #:
TI0466
Type:
Application Note
Categories:
Biochemicals and Metabolites
Compounds:
Creatinine and Purine Metabolites
Column(s):
Unison UK-Amino (150 x 4.6mm)

Creatinine and Purine Metabolites

Application #:
TI0464
Type:
Application Note
Categories:
Vitamins
Compounds:
25-Hydroxy vitamin D3 (Calcifediol)
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

LC-MS/MS Application for 25-Hydroxy Vitamin D3

Application #:
TI0462
Type:
Application Note
Categories:
Oligosaccharides
Biochemistry / Metabolism
Botanicals
Compounds:
Unsaturated Chondro-Disaccharides
Column(s):
Unison UK-Amino (250 x 3.0mm)

Unsaturated Chondro-Disaccharides

Application #:
TI0454
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
adenosine 3'
5'- cyclic monophosphate sodium (cAMP)
guanosine 3'
5'- cyclic monophosphate sodium (cGMP)
adenosine 5'- monophosphate disodium (AMP)
guanosine 5'- monophosphate disodium (GMP)
Column(s):
Unison UK-Amino (100 x 3.0mm)

Cyclic Mononucleotides

Application #:
TI0449
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
beta-nicotinamide adenine dinucleotide reduced form
disodium (NADH)
beta-nicotinamide adenine dinucleotide phosphate
reduced form
tetrasodium (NADPH)
beta-nicotinamide adenine dinucleotide phosphate
oxidized form
sodium (NADP)
beta-nicotinamide adenine dinucleotide oxidized form (NAD)
Column(s):
Unison UK-Amino (30 x 3.0mm)

Coenzymes (Nicotinamide adenine dinucleotides)

Application #:
TI0448
Type:
Application Note
Categories:
DNA Related Compounds
Biochemistry / Metabolism
Compounds:
adenosine 5'- triphosphate disodium (ATP)
adenosine 5'- monophosphate disodium (AMP)
adenosine 5'- diphosphate disodium (ADP)
Column(s):
Unison UK-Amino (30 x 3.0mm)

Adenosine Phosphates (AMP, ADP, ATP)

Application #:
TI0447
Type:
Application Note
Categories:
Saccharides
Biochemistry / Metabolism
Compounds:
D-fructose 6-phosphate disodium hydrate (F6P)
D-fructose 1
6-disphosphate trisodium octahydrate (F1
6P)
Column(s):
Unison UK-Amino (100 x 3.0mm)

Sugar Phosphates

Application #:
TI0446
Type:
Application Note
Categories:
Saccharides
Biochemistry / Metabolism
Compounds:
D-mannose 6-phosphate barium hydrate (M6P)
D-glucose 6-phosphate disodium (G6P)
D-fructose 6-phosphate disodium hydrate (F6P)
D-fructose 1-phosphate barium (F1P)
alpha-D-glucose 1-phosphate disodium hydrate (G1P)
alpha-D-galactose 1-phosphate dipotassium pentahydrate (Gal1P)
Column(s):
Unison UK-Amino (250 x 3.0mm)

Sugar Phosphates

Application #:
TI0429
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
N-glycolylneuraminic acid
N-acetylneuraminic acid
2-keto-D-gluconic acid hemicalcium salt hydrate
2-keto-3-deoxyoctonate ammonium salt
Column(s):
Unison UK-Amino (250 x 3.0mm)

Alpha-keto acids

Application #:
TI0421
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
PQQ ( pyrroloquinoline quinone )
Column(s):
Unison UK-Silica (150 x 2.0mm)
Unison UK-Amino (150 x 2.0mm)

PQQ: pyrroloquinoline quinone (Normal Phase)

Application #:
TI0417
Type:
Application Note
Categories:
Biochemistry / Metabolism
Pharmaceuticals & Cosmetics
Compounds:
theophylline
theobromine
caffeine
beta-(7-hydroxyethyl) theophylline
3-propylxanthine
1-methylxanthine
Column(s):
Unison UK-Amino (100 x 3.0mm)

Xanthines and Metabolites

Application #:
TI0416
Type:
Application Note
Categories:
Direct Serum Injection
Biochemistry / Metabolism
Compounds:
theophylline
theobromine
caffeine
beta-(7-hydroxyethyl) theophylline
3-propylxanthine
1-methylxanthine
Column(s):
Cadenza HS-C18 (150 x 3.0mm)

Xanthines and Metabolites in Bovine Serum

Application #:
TI0415
Type:
Application Note
Categories:
Biochemistry / Metabolism
Pharmaceuticals & Cosmetics
Compounds:
theophylline
theobromine
caffeine
beta-(7-hydroxyethyl) theophylline
3-propylxanthine
1-methylxanthine
Column(s):
Cadenza 5CD-C18 (75 x 2.0mm)
Cadenza CW-C18 (75 x 2.0mm)
Unison US-C18 (150 x 4.6mm)

Xanthines and Metabolites

Application #:
TI0409
Type:
Application Note
Categories:
Biochemistry / Metabolism
Pharmaceuticals & Cosmetics
Compounds:
uracil
dihydrouracil
dihydrofluorouracil
5-fluorouracil
Column(s):
Unison UK-Phenyl (250 x 4.6mm)
Unison UK-Amino (250 x 3.0mm)

5-Fluorouracil and Related Compounds

Application #:
TI0406
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
uridine
uracil
thymine
thymidine
guanosine
guanine
cytosine
cytidine
adenosine
adenine
Column(s):
Unison UK-Amino (250 x 3.0mm)

Nucleosides and Bases

Application #:
TI0397
Type:
Application Note
Categories:
LC-MS
Steroids
Biochemistry / Metabolism
Compounds:
testosterone
progesterone
pregnanetriol
pregnanediol
estrone
estriol
5-alpha-dihydrotestosterone
17-beta-estradiol
17-alphahydroxyprogesterone
Column(s):
Unison UK-C8 (50 x 2.0mm)

LC-MS/MS Application - Steroid Hormones and Metabolites

Application #:
TI0393
Type:
Application Note
Categories:
Amino Acids and Peptides
Biochemistry / Metabolism
Compounds:
glutathione reduced (GSH)
glutathione oxidized (GSSG)
Column(s):
Unison UK-Amino (100 x 4.6mm)

The Unison UK-Amino column shows excellent recognition of both the oxidized and reduced forms of glutathione.

Application #:
TI0379
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
nicotine
cotinine
anabasine hydrochloride
3-hydroxycotinine
Column(s):
Cadenza CD-C18 (100 x 3.0mm)
Unison UK-Amino (100 x 3.0mm)

Nicotine derivatives and metabolites can be separated by both reversed phase and normal phase chromatography. Reversed phase gives better run time.

Application #:
TI0377
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
L-carnitine
GABA
betaine
Column(s):
Unison UK-Amino (50 x 3.0mm)

Unison UK-Amino is able to separate amino acid-like molecules with good resolution.

Application #:
TI0376
Type:
Application Note
Categories:
Direct Serum Injection
Steroids
Biochemistry / Metabolism
Compounds:
beta-Estradiol 3
17-disulfate
beta-Estradiol 17-(beta-D-glucuronide)
17-beta-estradiol
Column(s):
Cadenza HS-C18 (150 x 3.0mm)

Cadenza HS-C18 successfully separates out small molecules like Estradiol and metabolites from bovine serum. The sample chromatogram looks almost identical to the standard chromatogram.

Application #:
TI0375
Type:
Application Note
Categories:
Steroids
Biochemistry / Metabolism
Compounds:
beta-Estradiol 3
17-disulfate
beta-Estradiol 17-(beta-D-glucuronide)
17-beta-estradiol
Column(s):
Cadenza CD-C18 (30 x 2.0mm)
Cadenza CW-C18 (30 x 2.0mm)
Cadenza CL-C18 (30 x 2.0mm)
Unison UK-C18 (30 x 2.0mm)
Unison UK-C8 (30 x 2.0mm)
Unison UK-Phenyl (30 x 2.0mm)

Many Cadenza and Unison columns achieve excellent resolution with beta-Estradiol 3,17-disulfate and 17beta-estradiol despite their similar structures.

Application #:
TI0371
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
propyl-L-arginine (N-PLA)
monomethyl-L-arginine (L-NMMA)
dimetyl-L-arginine (SDMA)
dimetyl-L-arginine (ADMA)
Column(s):
Unison UK-C18 (150 x 4.6mm)

Unison UK-C18 successfully separates methylated arginines of similar structure. Compounds are pre-column derivatized to enable fluroescence detection.

Application #:
TI0370
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
6-nitronorepine
6-nitroepinephrine
6-nitrodopamine
Column(s):
Unison UK-C18 (250 x 4.6mm)

Unison UK-C18 successfully separates nitroNE, nitroE, and nitroDA. Detection is via post-column derivatization.

Application #:
TI0362
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
norepinephrine
epinephrine
dopamine
Column(s):
Unison UK-Amino (30 x 3.0mm)

Unison UK-Amino column separates catecholamines of similar structure using normal phase/anion exchange mode.

Application #:
TI0360
Type:
Application Note
Categories:
Biochemistry / Metabolism
Food Chemistry
Compounds:
Taurine in nutritious supplement drink
Column(s):
Unison UK-Amino (100 x 3.0mm)

Unison UK-Amino column separates out taurine in a drink mixture.

Application #:
TI0357
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
vanillylmandelic acid (VMA)
mandelic acid
homovanillic acid (HVA)
Column(s):
Unison UK-Amino (150 x 4.6mm)

Mandelic acid and related compounds

Application #:
TI0345
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
p-methylhippuric acid
o-methylhippuric acid
m-methylhippuric acid
hippuric acid
Column(s):
Unison UK-Amino (250 x 4.6mm)

Hippuric acids

Application #:
TI0339
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
creatinine
creatine phosphate
creatine
Column(s):
Unison UK-Amino (150 x 2.0mm)

Creatine and metabolites

Application #:
TI0338
Type:
Application Note
Categories:
Biochemistry / Metabolism
LC-MS
Compounds:
delta-9tetrahydrocannabinol
11-hydroxy-delta-9-tetrahydrocannabinol
Column(s):
Unison US-C18 (50 x 2.0mm)

LC-MS of marajuana and its metabolite in blood

Application #:
TI0333
Type:
Application Note
Categories:
Saccharides
Biochemistry / Metabolism
Compounds:
N-acetylneuraminic acid (sialic acid)
D-glucuronic acid
D-galacturonic acid
Column(s):
Unison UK-Amino (250 x 3.0mm)

Sialic acid and uronic acids

Application #:
TI0257
Type:
Application Note
Categories:
Biochemistry / Metabolism
Compounds:
uric acid
oxalic acid
L-ascorbic acid
Column(s):
Unison UK-C18 (250 x 4.6mm)

Causative Substances of Urolithiasis

Application #:
TI0247
Type:
Application Note
Categories:
Column Performance
Pharmaceuticals & Cosmetics
Biochemistry / Metabolism
Compounds:
procainamide
N-acetylprocainamide
Column(s):
Cadenza CD-C18 (30 x 4.6mm)
Cadenza CL-C18 (30 x 4.6mm)

Separation properties of basic compounds on CL-C18

Application #:
TI0246
Type:
Application Note
Categories:
Column Performance
Biochemistry / Metabolism
Food Chemistry
Pharmaceuticals & Cosmetics
Compounds:
benzoic acid
salicylic acid
theophylline
phenol
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)

Separation properties for acidic compounds on CL-C18

Application #:
TI0245
Type:
Application Note
Categories:
Column Performance
Biochemistry / Metabolism
Compounds:
theophylline
phenol
caffeine
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

Retention behavior of polar compounds

Application #:
TI0241
Type:
Application Note
Categories:
Dihydrouracil
Compounds:
‚„ihydrouracil
uracil
Column(s):
Cadenza CD-C18 (250 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Application #:
TI0227
Type:
Application Note
Categories:
ELSD
Compounds:
taurine
thiamine (B1)
pyridoxine (B6)
nicotinamide (B3)
Column(s):
Unison UK-C18 (75 x 4.6mm)

LC-ELSD of taurine and water soluble vitamins

Application #:
TI0226
Type:
Application Note
Categories:
Creatine Metabolites
Compounds:
creatine
creatine phosphate
creatinine
Column(s):
Unison UK-C18 (250 x 4.6mm)

Separation of creatine and related polar metabolites with 100% aqueous mobile phase, using the UK-C18, which excels in the separation of highly polar compounds.

Application #:
TI0195
Type:
Application Note
Categories:
Coenzyme Q10
Compounds:
coenzymeQ10 (ubiquinone-10)
Column(s):
Cadenza CD-C18 (75 x 3.0mm)
Cadenza CL-C18 (75 x 3.0mm)
Unison UK-C18 (75 x 3.0mm)
Unison UK-C8 (75 x 3.0mm)
Unison UK-Silica (150 x 3.0mm)

Analysis of coenzyme Q10, a dietary supplement used for its antioxidant action, with various columns.

Application #:
TI0193
Type:
Application Note
Categories:
GDP-Sugars (Sugar-nucleotides)
Compounds:
GDP-mannose
GDP-galactose
Column(s):
Unison UK-C18 (250 x 4.6mm)

Application #:
TI0192
Type:
Application Note
Categories:
UDP-Sugars (Sugar-nucleotides)
Compounds:
UDP-arabinose
UDP-glucose
UDP-xylose
Column(s):
Unison UK-C18 (250 x 4.6mm)

Separation of UDP-sugars, intermediates in sugar chain synthesis, the molecular structure of which differs only in the sugar group.

Application #:
TI0191
Type:
Application Note
Categories:
Betaine
Compounds:
betaine
Column(s):
Unison UK-Silica (250 x 3.0mm)

Reverse phase analysis of the highly polar compound betaine, which is quite difficult to retain in normal phase analysis.

Application #:
TI0190
Type:
Application Note
Categories:
Short-Chain Fatty Acids
Compounds:
n-propionic acid
n-butyric acid
n-valeric acid
n-caproic acid
Column(s):
Unison UK-C18 (50 x 4.6mm)
Unison UK-C8 (50 x 4.6mm)
Unison UK-Phenyl (50 x 4.6mm)

Separation of short chain cable fatty acids without modifying the functional group.

Application #:
TI0184
Type:
Application Note
Categories:
Gamma-Aminobutyric Acid
Compounds:
gamma-aminobutyric acid
Column(s):
Unison UK-C18 (75 x 4.6mm)
Unison UK-Silica (250 x 2.0mm)

Normal phase separation of the neurotransmitter GABA.

Application #:
TI0181
Type:
Application Note
Categories:
Xanthine Oxidase Related Compounds
Compounds:
uric acid
hypoxanthine
xanthine
allopurinol
Column(s):
Unison UK-C18 (75 x 4.6mm)

This analysis of xanthine oxydase related compounds includes separation of its substrate, product and inhibitor.

Application #:
TI0180
Type:
Application Note
Categories:
Nucleotides
Compounds:
ATP
ADP
AMP
Column(s):
Unison UK-Silica (250 x 3.0mm)

Analysis of ATP, ADP and AMP in aqueous normal phase using a silica column.

Application #:
TI0179
Type:
Application Note
Categories:
Purine Nucleotide Metabolites (Reversed-Phase)
Compounds:
inosine
hypoxanthine
guanine
xanthine
uric acid
allantoin
allantoic acid
glyoxylic acid
urea
Column(s):
Unison UK-C18 (250 x 3.0mm)

Reverse phase analysis of purine nucleotide metabolites, including uric acid and allantoin.

Application #:
TI0178
Type:
Application Note
Categories:
Purine Nucleotide Metabolites (Normal Phase)
Compounds:
inosine
Column(s):
Unison UK-Silica (250 x 3.0mm)

Normal phase analysis of purine nucleotide metabolites, including uric acid and allantoin, using a silica column.

Application #:
TI0174
Type:
Application Note
Categories:
Catecholamines
Compounds:
L-norepinephrine
octopamine
L-epinephrine
dopamine
serotonin
Column(s):
Unison UK-C18 (75 x 4.6mm)

Analysis of the highly polar catecholamines. The ion pair reagent, not included, is separated by the mobile phase.

Application #:
TI0170
Type:
Application Note
Categories:
L-Carnitine
Compounds:
L-carnitine
Column(s):
Unison UK-C18 (75 x 4.6mm)
Unison UK-Silica (150 x 3.0mm)

The polar compound L-Carnitine, analyzed in both normal and reverse phase modes.

Application #:
TI0151
Type:
Application Note
Categories:
Caproic Acid and Isomers
Compounds:
3-methylvaleric acid
2-methylvaleric acid
4-methylvaleric acid
n-caproic acid
Column(s):
Unison UK-C18 (250 x 4.6mm)
Unison UK-C18 (500 x 4.6mm)

Caproic acid and its methylvaleric acid isomers are separated with high resolution using our 500mm long column.

Application #:
TI0132
Type:
Application Note
Categories:
Urea
Compounds:
urea
Column(s):
Unison UK-C18 (250 x 4.6mm)

Reverse phase analysis of dye-labeled urea, a highly polar compound.

Application #:
TI0131
Type:
Application Note
Categories:
TCA cycle
Compounds:
oxalacetic acid
malic acid
alpha-ketoglutaric acid
succinic acid
cis-aconitic acid
fumaric acid
isocitric acid
citric acid
pyruvic acid
Column(s):
Unison UK-Phenyl (250 x 4.6mm)

Simultaneous analysis of Citric Acid Cycle compounds using our phenyl column.

Application #:
TI0130
Type:
Application Note
Categories:
TCA cycle
Compounds:
oxalacetic acid
malic acid
alpha-ketoglutaric acid
succinic acid
Column(s):
Unison UK-C18 (250 x 4.6mm)

Simultaneous analysis of Citric Acid Cycle compounds using our UK-C18 column.

Application #:
TI0129
Type:
Application Note
Categories:
Ornithine Cycle
Compounds:
urea
fumaric acid
citrulline
ornithine
argininosuccinic acid
arginine
Column(s):
Unison UK-C18 (250 x 4.6mm)

Simultaneous analysis of Ornithine Cycle comounds using our UK-C18, which has affinity for highly polar compounds.

Application #:
TI0127
Type:
Application Note
Categories:
Fatty Acid ADAM Derivatives
Compounds:
caproic acid
caprylic acid
pelargonic acid
capric acid
lauric acid
alpha-linolenic acid
myristic acid
linoleic acid
pentadecanoic acid
palmitic acid
oleic acid
stearic acid
nonadecanoic acid
arachidic acid
Column(s):
Unison UK-C8 (150 x 4.6mm)

High-speed separations with Unison UK-C8 150mm column compared with a 5um 250mm column popular on the market.

Application #:
TI0115
Type:
Application Note
Categories:
Column Performance
Biochemistry / Metabolism
Compounds:
Theophylline
phenol
caffeine
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

Retention properties of polar compounds

Application #:
TI0053
Type:
Application Note
Categories:
Creatine in Chewing Gum
Compounds:
creatine
Column(s):
Cadenza CD-C18 (100 x 4.6mm)

Analysis of creatine in chewing gum

Application #:
TI0047
Type:
Application Note
Categories:
Inosinic acid and metabolites
Compounds:
hypoxanthine
inosine
inosine 5'-monophosphate (IMP)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Separation of inosinic acid, a flavor enhancer.

Application #:
TI0012
Type:
Application Note
Categories:
Compounds:
mandelic acid
hippuric acid
o-metylhippuric acid
p-metylhippuric acid
m-metylhippuric acid
Column(s):
Cadenza CD-C18 (250 x 4.6mm)

Separation of hippuric acid, a metabolite of toluene and xylene. Cadenza can recognize positional isomers of methyl hippuric acid as well.

Application #:
SP0028
Type:
Poster
Categories:
Biochemistry/Metabolism
Column Performance
Pharmaceuticals and Cosmetics
Amino Acids and Peptides
Compounds:
nicotinic acid
tryptophan
bromoguanosine
labetalol
aspirin
fexofenadine
reserpine
SB243213A
ibuprofen
penicillin V
Column(s):

An Evaluation of a Multi-Mode Stationary Phase for HPLC Separations Using a Multi-Component Test Mix

Application #:
SP0027
Type:
Poster
Categories:
Biochemistry/Metabolism
Food Chemistry
Saccharides
Vitamins
Compounds:
glycerol
rhamnose
erythritol
arabinose
xylose
fructose
sorbitol
mannose
galactose
glucose
inositol
sucrose
maltulose
lactose
maltose
trehalose
raffinose
maltotriose
nystose
maltotetraose
vitamin B1
vitamin B8
vitamin B6
vitamin C
vitamin B5
vitamin B9
vitamin B12
vitamin B2
vitamin B7
vitamin A
vitamin K2
vitamin D2
vitamin D3
vitamin E
vitamin K1
quinic acid
malic acid
tartaric acid
succinic acid
citric acid
gallic acid
lactic acid
protocatechuic acid
4-HPAC
chlorogenic acid
syringic acid
gentistic acid
Column(s):

Advantage of a New Generation of Evaporative Light-Scattering Detectors: Universality, Higher Sensitivities and Cost-Effectiveness for Multi-Element Analyses in Liquid Chromatography - An Application Review

Application #:
SP0025
Type:
Poster
Categories:
LC-MS
Biochemistry/Metabolism
Steroids
Compounds:
DHEA-P
DHT-S
testosterone-P
pregnenolone-S
DHT-P
DHEA-A
pregnenolone-P
testosterone-A
DHEA-S
DHT-A
testosterone-S
pregnenolone-A
ubiquinone Q2
leukotriene A4
5 HEPE
epipregnanolone
alloepipregnanolone
allopregnanlone
melanin
resolvin D1
resolvin D2
18-oxocortisol
7a-OH-5b-cholanic acid
12b-OH-5b-cholanic acid
lithocholic acid
isolithocholic acid
isoallolithocholic acid
allolithocholic acid
allodeoxycholic acid
isodeoxycholic acid
deoxycholic acid
ursodeoxycholic acid
murocholic acid
hyodeoxycholic acid
3a
7a-dihydroxycholanoic acid
3b
7a-dihydroxy-5b-cholanoic acid
3b
12a-dihydroxy-5b-cholanoic acid
3a
12b-dihydroxy-5b-cholanoic acid
3b
12a-dihydroxy-5a-cholanoic acid
3b
12b-dihydroxy-5b-cholanoic acid
allochenodeoxycholic acid
chenodeoxycholic acid
isoursodeoxycholic acid
isohyodeoxycholic acid
7b
12a-dihydroxycholanoic acid
Column(s):

High-throughput non-targeted metabolic profiling with hybrid stationary phase LC column coupled to Q-TOF-MS in cancer research

Application #:
SP0015
Type:
Poster
Categories:
0
Compounds:
0
Column(s):
Cadenza CL-C18 (0 x 0mm)

Ultra High Sensitivity Analysis of Testosterone

Application #:
JA0006
Type:
Paper
Categories:
0
Compounds:
0
Column(s):
Unison UK-Silica (0 x 0mm)

Spin Tip Solid-Phase Extraction And HILIC-MS-MS For Quantitative Determination Of Methamphetamine And Amphetamine In Human Plasma

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