LC-MS: Cholane derivatives (Cholanic acids)

LC-MS: Cholane derivatives (Cholanic acids)

Cholesterol related compounds

Transferrin

Transferrin

Transferrin

Transferrin

LC-MS: Alkylphosphates and alkylphosphonates

LC-MS: Melatonin related compounds

LC-MS: Melatonin related compounds

LC-MS: Melatonin related compounds

Melatonin related compounds

Melatonin related compounds

LC-MS: Bilirubin (Heme metabolite)

LC-MS: Styrene urinary metabolites

LC-MS: Styrene urinary metabolites

Styrene urinary metabolites

Styrene urinary metabolites

Thymine, Ethyl ascorbic acid

Bilirubin (Heme metabolite)

Androstenedione biosynthetic pathway

LC-MS: unsaturated chondro-disaccharides

LC-MS/MS: Isomer separation of central metabolites using the multi-modal properties of a hybrid column

LC-MS: C-protected amino acids

LC-MS: Adenosine phosphates

Oligonucleotides (Oligothymidylic acid)

LC-MS: Tyrosinemia type 1 related compounds

LC-MS: Uridine isomer (psi-uridine)

Methylhippuric acid isomers

Glycogen

LC-MS: Nucleoside triphosphate (NTP)

LC-MS: Nucleoside triphosphate (NTP)

Glycogen

SEC: Glycogen

Galactosylceramide from bovine brain

Galactosylceramide from bovine brain

Galactosylceramide from bovine brain

Galactosylceramide from bovine brain

Galactosylceramide from bovine brain

Galactosylceramide from bovine brain

Lipo-Oxytocin-1 (LOT-1)

LC-MS: HbA1c and AGEs related amadori compounds

LC-MS: HbA1c and AGEs related amadori compounds

LC-MS: 4-Methylmidazole in Cola and Instant Coffee

LC-MS: 4-Methylmidazole in Dark Beer

LC-MS: Strong organic base catalysts

LC-MS: Methylmidazole isomers

LC-MS: Sugar phosphates

LC-MS: Plant hormones

LC-MS: Coenzymes (Nicotinamide adenine dinucleotides)

LC-MS: Organic acids in Soy sauce

LC-MS: Oxalic acid

LC-MS: hydroxycarboxylic acids

LC-MS: Alcohol biomarkers (EtG, EtS)

LC-MS: Acetic acid and formic acid

LC-MS: Methylmalonic acid (sample solvent effect)

LC-MS: 44 Organic Acids Analysis (IEX mode)

LC-MS: Batch-to-batch reproducibility

LC-MS: Adenosine nucleotides and phosphoric acid

LC-MS: TCA (tricarboxylic acid) cycle / Citric acid cycle

LC-MS: Intact Organic Acid Analysis (IEX)

LC-MS: Selenoamino acids

LC-MS: Cysteine derivatives

LC-MS: Ascorbic acid and related compounds

LC-MS: Peptides (Angiotensin)

LC-MS: Asymmetric / symmetric dimethylarginines (ADMA/SDMA) and related compounds

LC-MS: Oxytocin (OXT, peptide hormone)

SEC: Ribonuclease A (RNase A), from bovine pancreas

LC-MS: Urocanic acid and related amino acids

Metal-Free Column: phosphoserine

Metal Free Column: Nucleoside triphosphate (NTP), Trinucleotide

LC-MS: Citrulline-NO cycle

LC-MS: 5-Aminolevulinic acid (ALA) and a precursor Glycine

LC-TOFMS: Urinary compounds

LC-TOFMS: Coenzymes

LC-TOFMS: Methylfolate

LC-TOFMS: Glutathione

LC-TOFMS: Cytosine compounds

LC-TOFMS: Creatine compounds

LC-TOFMS: Choline compounds

SEC: IgG, from human serum

SEC: Poly-gamma-glutamic acid

SEC: Proteoglycan

SEC: Ovalbumin

SEC: Myoglobin, from equine skeletal muscle

SEC: Insulin, from bovine pancreas

SEC: Alpha-lactalbumin, from bovine milk

SEC: Alpha-Casein, from bovine milk

SEC: Alpha-chymotrypsinogen A, from bovine pancreas

SEC: Xthantine oxidase (XOD), from Buttermilk

SEC: Beta - Glucosidase

SEC: Alpha - Amylase

Histamine

LC-MS: Thyroid hormone

LC-MS: Thyroid hormone

LC-MS: Orexin A and B

LC-MS: Orexin A and B

LC-MS: Orexin A and B

LC-MS: Kynurenine metabolism pathway

LC-MS: SAH and SAM

LC-MS: Acidic compound (mobile phase pH)

LC-MS: Insulin (Mobile phase pH)

LC-MS: Amyloid beta-peptide [Human,1-40] (Mobile phase pH)

LC-MS: Amyloid beta-peptide [Human,1-40] (alkali conditions)

Lipid A (Salmonella minnesota Re595)

Recombinant human serum albumin (rHSA)

Recombinant and biosynthetic human serum albumin (HSA)

Recombinant human serum albumin (rHSA)

LC-MS: retro-dipeptide isomers

LC-MS, Biochemistry/Metabolism, Amino Acids and Peptides

LC-MS: choline metabolites and related compounds

LC-MS: isoproterenol

LC-MS: carnitine related compounds

LC-MS: Amino acids in human serum (HClO4)

LC-MS: Amino acids in human serum (SSA)

LC-MS: dipeptides

LC-MS: Epinephrine and metanephrine

LC-MS: Synthetic polyamines

LC-MS: Biogenic polyamines

LC-MS/MS: Acetylcholine and d4-acetylcholine

LC-MS: Neuroblastoma related compounds

LC-MS: Neurotransmitters (2)

The Unison UK-Amino column successfully separates adenosine and inosine, which are very similar in structure.

The Scherzo SM-C18 and Unison UK-Amino columns successfully separate the small, polar ascorbic acid related compounds in normal phase mode.

The Scherzo SS-C18 column provides better retention of Shikimic Acid than a standard C18 column.

Cadenza CW-C18, Unison UK-C18, and Unison UK-C8 are all able to resolve the very polar and charged Acetyl CoA and CoA, differing only in an acetyl group.

The Scherzo SM-C18 and Scherzo SS-C18 column resolve very polar carboxylic acids that normally co-elute on a standard C18 column (Unison UK-C18).

The Scherzo SS-C18 column retains Prostaglandins the most, followed by Scherzo SM-C18 and Scherzo SW-C18.

Under neutral pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

Under acidic pH conditions, columns in the Unison and Cadenza series have similar retention and resolution properties.

On the Unison UK-C18 column, tetrahydrofuran gives better resolution of Prostaglandins than acetonitrile and acetone.

Acetonitrile and methanol have opposite retention properties in the separation of prostaglandins on the Unison UK-C18 column.

The Scherzo SM-C18 column successfully resolves nucleotide triphosphates of similar structure.

The Scherzo SM-C18 column can separate these two molecules, which would normally coelute on a standard C18 column.

The Scherzo SM-C18 and the Scherzo SW-C18 show similar retention of these small molecules as the standard Unison UK-C18. However, the Scherzo SS-C18 shows the best resolution between these three compounds.

The Scherzo SS-C18 column retains and separates glutamic acid and GABA better than a conventional C18 column

Small, polar molecules like neurotransmitters are difficult to retain on a conventional C18 column like Unison UK-C18. Scherzo SS-C18 can retain these compounds much better than a standard Unison UK-C18 column, as well as resolve the peaks better. Conditions compatible with LC-MS

The highly charged phosphate groups make separation of AMP, ADP, and ATP difficult on a conventional C18 column. Scherzo SW-C18 is able to elute these compounds effectively, in both ammonium acetate and ammonium phosphate buffers.

Cytochrome c

Serotonin related compounds

Histamine related compounds

Neurotransmitters

Zwitterionic compounds

Zwitterionic compounds

Catecholamines

Exploring different C18 stationary phases for metabolites profiling

Simulatneous HPLC/LT-ELSD Analysis of Organic and Phenolic Acids

Simulatneous HPLC/LT-ELSD Analysis of Water Soluble and Fat Soluble Vitamins

Scherzo is able to separate mandelic acid and dopamine, which coelute on a conventional C18 column (Unison UK-C18). This is possible because the ionic ligands recognize the small structural differences between the two compounds.

Catecholamine metabolites

The effect of gradient slope and column length for thyroglobulin separation

The effect of gradient slope for insulin separation

The effect of gradient slope for angiotensin II serparation

Unsaturated Chondro-Disaccharides

LC-MS(MRM) Application for Monosaccharides

Organic acids (Anion exchange mode)

Urea and related compounds

Coenzymes (Nicotinamide adenine dinucleotides)

Coenzymes (Nicotinamide adenine dinucleotides)

Cyclic mononucleotides (cAMP, cGMP)

Organic acids (2)

Organic Acids

Purine bases

Methimazole and potential metabolites

Levulinic Acid, Lactic Acid and methyl Esters

Adenosine Phosphates

Anserine and Related Compounds

Mononucleotides

Glutathiones

Sodium Chloride

Mevalonolactone and Mevalonic Acid

Organic Acids

Estradiol and Metabolites

Identification of sphingomyelin molecular species with LC-MS/MS

LC-Q-TOF-MS application for high-throughput non-targeted metabolomics with a hybrid ODS column

LC-MS/MS Application for Sphingolipids (Sphinganine-1-PO4)

LC-MS/MS Application for Sphingolipids (Sphingosine-1-PO4)

LC-MS/MS Application for Sphingolipids (Sphingosine)

LC-MS/MS Application for Sphingolipids (Sphinganine)

LC-MS/MS Application for Sphingolipids (Ceramide)

LC-MS Application for Phospholipids

Creatinine and Purine Metabolites

LC-MS/MS Application for 25-Hydroxy Vitamin D3

Unsaturated Chondro-Disaccharides

Cyclic Mononucleotides

Coenzymes (Nicotinamide adenine dinucleotides)

Adenosine Phosphates (AMP, ADP, ATP)

Sugar Phosphates

Sugar Phosphates

Alpha-keto acids

PQQ: pyrroloquinoline quinone (Normal Phase)

Xanthines and Metabolites

Xanthines and Metabolites in Bovine Serum

Xanthines and Metabolites

5-Fluorouracil and Related Compounds

Nucleosides and Bases

LC-MS/MS Application - Steroid Hormones and Metabolites

The Unison UK-Amino column shows excellent recognition of both the oxidized and reduced forms of glutathione.

Nicotine derivatives and metabolites can be separated by both reversed phase and normal phase chromatography. Reversed phase gives better run time.

Unison UK-Amino is able to separate amino acid-like molecules with good resolution.

Cadenza HS-C18 successfully separates out small molecules like Estradiol and metabolites from bovine serum. The sample chromatogram looks almost identical to the standard chromatogram.

Many Cadenza and Unison columns achieve excellent resolution with beta-Estradiol 3,17-disulfate and 17beta-estradiol despite their similar structures.

Unison UK-C18 successfully separates methylated arginines of similar structure. Compounds are pre-column derivatized to enable fluroescence detection.

Unison UK-C18 successfully separates nitroNE, nitroE, and nitroDA. Detection is via post-column derivatization.

Unison UK-Amino column separates catecholamines of similar structure using normal phase/anion exchange mode.

Unison UK-Amino column separates out taurine in a drink mixture.

Mandelic acid and related compounds

Hippuric acids

Creatine and metabolites

LC-MS of marajuana and its metabolite in blood

Sialic acid and uronic acids

Causative Substances of Urolithiasis

Separation properties of basic compounds on CL-C18

Separation properties for acidic compounds on CL-C18

Retention behavior of polar compounds

LC-ELSD of taurine and water soluble vitamins

Separation of creatine and related polar metabolites with 100% aqueous mobile phase, using the UK-C18, which excels in the separation of highly polar compounds.

Analysis of coenzyme Q10, a dietary supplement used for its antioxidant action, with various columns.

Separation of UDP-sugars, intermediates in sugar chain synthesis, the molecular structure of which differs only in the sugar group.

Reverse phase analysis of the highly polar compound betaine, which is quite difficult to retain in normal phase analysis.

Separation of short chain cable fatty acids without modifying the functional group.

Normal phase separation of the neurotransmitter GABA.

This analysis of xanthine oxydase related compounds includes separation of its substrate, product and inhibitor.

Analysis of ATP, ADP and AMP in aqueous normal phase using a silica column.

Reverse phase analysis of purine nucleotide metabolites, including uric acid and allantoin.

Normal phase analysis of purine nucleotide metabolites, including uric acid and allantoin, using a silica column.

Analysis of the highly polar catecholamines. The ion pair reagent, not included, is separated by the mobile phase.

The polar compound L-Carnitine, analyzed in both normal and reverse phase modes.

Caproic acid and its methylvaleric acid isomers are separated with high resolution using our 500mm long column.

Reverse phase analysis of dye-labeled urea, a highly polar compound.

Simultaneous analysis of Citric Acid Cycle compounds using our phenyl column.

Simultaneous analysis of Citric Acid Cycle compounds using our UK-C18 column.

Simultaneous analysis of Ornithine Cycle comounds using our UK-C18, which has affinity for highly polar compounds.

High-speed separations with Unison UK-C8 150mm column compared with a 5um 250mm column popular on the market.

Retention properties of polar compounds

Analysis of creatine in chewing gum

Separation of inosinic acid, a flavor enhancer.

Separation of hippuric acid, a metabolite of toluene and xylene. Cadenza can recognize positional isomers of methyl hippuric acid as well.