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Technical Information   Posters   Papers

Application #:
TI0930
Type:
Application Note
Categories:
LC-MS
Pharmaceuticals and Cosmetics
Column Performance
Compounds:
bifenthrin
etoxazole
fipronil
pridaben
Column(s):
Cadenza CD-C18 (50 x 2.0mm)
Unison UK-C18 (50 x 2.0mm)

LC-MS/MS: Insecticides

Application #:
TI0928
Type:
Application Note
Categories:
LC-MS
Pharmaceuticals and Cosmetics
Column Performance
Compounds:
Fipronil
Column(s):
Cadenza CD-C18 (50 x 2.0mm)
Unison UK-C18 (50 x 2.0mm)

LC-MS/MS: Insecticide (Fipronil)

Application #:
TI0915
Type:
Application Note
Categories:
Synthetic Polymers
Pharmaceuticals and Cosmetics
Column Performance
Compounds:
Poly(lactic-co-glycolic acid) (PLGA)
Column(s):
(250 x 4.6mm)

SEC: PLGA, Poly(lactic-co-glycolic acid)

Application #:
TI0913
Type:
Application Note
Categories:
Synthetic Polymers
Column Perfomance
Compounds:
Polylactide (PLA)
Column(s):
(250 x 4.6mm)

SEC: Polylactide (PLA)

Application #:
TI0879
Type:
Application Note
Categories:
LC-MS
Food Chemistry
Botanicals
Amino Acids and Peptides
Column Performance
Compounds:
Allicin
diallyl disulfide
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)

Allicin and diallyl disulfide in Garlic

Application #:
TI0878
Type:
Application Note
Categories:
LC-MS
Food Chemistry
Botanicals
Amino Acids and Peptides
Column Performance
Compounds:
Alliin
S-allyl-L-cysteine
Column(s):
Intrada Amino Acid (100 x 3.0mm)
Scherzo SS-C18 (100 x 3.0mm)

Alliin and S-allyl-L-cysteine in Garlic

Application #:
TI0872
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
monoclonal antibody (mAb)
Column(s):
(250 x 4.6mm)

SEC: Monoclonal antibody (mAb)

Application #:
TI0870
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
hemoglobin
Column(s):
(250 x 4.6mm)

SEC: Hemoglobin

Application #:
TI0868
Type:
Application Note
Categories:
Synthetic Polymers
Column Performance
Compounds:
Polystyrene
Column(s):
(250 x 4.6mm)

SEC: Polystyrene

Application #:
TI0865
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
thyroglobulin
Column(s):
(250 x 4.6mm)

SEC separation: Thyroglobulin (Tg)

Application #:
TI0864
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
thyroglobulin
IgG
ovalbumin
insulin
Column(s):
(250 x 4.6mm)

SEC separation for proteins (column connection)

Application #:
TI0863
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
BSA Monomer 66 kDA
BSA Dimer 132 kDa
Column(s):
(250 x 4.6mm)

BSA (Bovine Serum Albumin) Monomer, Dimer

Application #:
TI0856
Type:
Application Note
Categories:
LC-MS
Industrial Chemical
Column Performance
Compounds:
Bromate
Nitrate
Chlorate
Chlorine
Sulfate
Column(s):
Scherzo SS-C18 (100 x 3.0mm)

LC-MS: Bromate and anions

Application #:
TI0854
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Column Performance
Compounds:
triiodothyronine (T3)(L-3
3'
5-triiodothyronine)
thyroxine (T4)(L-3
5
3'
5'-tetraiodothyronine)
Column(s):
Scherzo SM-C18 (50 x 3.0mm)
Cadenza CW-C18 (50 x 3.0mm)
Unison UK-C18 (50 x 3.0mm)
Unison UK-C8 (50 x 3.0mm)
Unison UK-Phenyl (50 x 3.0mm)

LC-MS: Thyroid hormone

Application #:
TI0834
Type:
Application Note
Categories:
Column Performance
LC-MS
Biochemistry/Metabolism
Compounds:
cholic acid
Column(s):

LC-MS: Acidic compound (mobile phase pH)

Application #:
TI0833
Type:
Application Note
Categories:
Column Performance
Pharmaceuticals and Cosmetics
Compounds:
methylparaben
ethylparaben
isopropylparaben
propylparaben
isobutylparaben
butylparaben
Column(s):

Paraben isomers

Application #:
TI0831
Type:
Application Note
Categories:
Column Performance
LC-MS
Amino Acids and Peptides
Biochemistry/Metabolism
Compounds:
Insulin
Column(s):

LC-MS: Insulin (Mobile phase pH)

Application #:
TI0830
Type:
Application Note
Categories:
Column Performance
LC-MS
Amino Acids and Peptides
Biochemistry/Metabolism
Compounds:
Amyloid beta-peptide [Human
1-40]
Column(s):

LC-MS: Amyloid beta-peptide [Human,1-40] (Mobile phase pH)

Application #:
TI0829
Type:
Application Note
Categories:
Column Performance
LC-MS
Amino Acids and Peptides
Biochemistry/Metabolism
Compounds:
Amyloid beta-peptide [Human
1-40]
Column(s):

LC-MS: Amyloid beta-peptide [Human,1-40] (alkali conditions)

Application #:
TI0828
Type:
Application Note
Categories:
LC-MS
Column Performance
Pharmaceuticals and Cosmetics
Compounds:
doxepine
Column(s):

LC-MS: Basic compound (mobile phase pH)

Application #:
TI0827
Type:
Application Note
Categories:
LC-MS
Column Performance
Pharmaceuticals and Cosmetics
Compounds:
scoporamine
physostigmine
Column(s):

LC-MS: Alkaloids (alkali conditions)

Application #:
TI0826
Type:
Application Note
Categories:
Column Performance
Pharmaceuticals and Cosmetics
Compounds:
doxepine
imipramine
trimipramine
Column(s):

Tricyclic antidepressants

Application #:
TI0821
Type:
Application Note
Categories:
Biochemistry/Metabolism
Proteins
Column Performance
Compounds:
Recombinant human serum albumin (rHSA)
Column(s):
Intrada WP-RP (250 x 4.6mm)
Cadenza CW-C18 (250 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

Recombinant human serum albumin (rHSA)

Application #:
TI0803
Type:
Application Note
Categories:
LC-MS
Biochemistry/Metabolism
Column Performance
Compounds:
acetylcholine
d4-acetylcholine
Column(s):
Scherzo SS-C18 (50 x 2.0mm)

LC-MS/MS: Acetylcholine and d4-acetylcholine

Application #:
TI0709
Type:
Application Note
Categories:
Pharmaceutical and Cosmetics
DNA related compounds
Column Performance
Compounds:
uracil
isopropylparaben
propylparaben
butylparaben
Column(s):
Cadenza CD-C18 (100 x 4.6mm)

The Cadenza CD-C18 HT 3 micron column shows comparable resolution to a sub-2 micron column, with much lower pressure.

Application #:
TI0708
Type:
Application Note
Categories:
Pharmaceutical and Cosmetics
DNA related compounds
Column Performance
Compounds:
uracil
isopropylparaben
propylparaben
butylparaben
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

The Cadenza CD-C18 HT 3 micron column shows better resolution between isopropylparaben and propylparaben than a sub-2 micron column, and the CD-C18 HT column also shows a higher plate count.

Application #:
TI0707
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
DNA related compounds
Column Performance
Compounds:
uracil
isopropylparaben
propylparaben
butylparaben
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

The Cadenza CD-C18 HT 3 micron column shows lower pressure, higher plate count, and higher resolution than a sub-2 micron column.

Application #:
TI0652
Type:
Application Note
Categories:
Column Performance
Biochemistry/Metabolism
LC-MS
Compounds:
alanine
N
N-dimethylglycine
serine
fumaric acid
succinic acid
cysteine
oxaloacetic acid
Gly-Gly
malic acid
alpha-ketoglutarate
citric acid
2-methylhippuric acid
gamma-D-glutamylglycine
salbutomal
AMP
ribo-avin
Phe-gly-phe-gly
s-(p-nitrobenzyl) glutathione
s-(p-azidophenacyl)glutathione
taurocholic acid
O-beta-D-glucuronosyl-naphthol AS-BI
Leucine enkephalin
Arg-Pro-Pro-Gly-Phe
Reserpine
GSSG
Arg-Lys-Asp-Val-Tyr
Coenzyme A
Acetyl CoA
Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr (peptide T)
bradykinin fragment 1-8
Tyr-tyr-tyr-tyr-tyr-tyr
Column(s):
Scherzo SM-C18 (150 x 2.0mm)

Exploring different C18 stationary phases for metabolites profiling

Application #:
TI0644
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
albumin (eggs)
beta-glucosidase (almond)
thyroglobulin (porcin thyroid)
lectin (concanavalin A from Jack bean)
alpha-chymotryosinogen A (bovine pancreas)
monoclonal antibody (anti-AFP)
myoglobin (equine skeletal muscle)
alpha-lactalbumin (bovine milk)
papin (papaya latex)
albumin (human serum)
lactoferrin (bovine milk)
lysozyme (chicken egge white)
cytochrome c (horse heart)
Column(s):
Presto FF-C18 (75 x 4.6mm)

Protein separation on non-porous ODS column

Application #:
TI0643
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
albumin (eggs)
beta-glucosidase (almond)
thyroglobulin (porcin thyroid)
lectin (concanavalin A
from Jack bean)
alpha-chymotryosinogen A (bovine pancreas)
myoglobin (equine skeletal muscle)
alpha-lactalbumin (bovine milk)
apain (papya latex)
albumin (human serum)
lactoferrin (bovine milk)
lysozyme (chicken egg white)
cytochrome c (horse heart)
myosin (rabbit muscle)
alpha0casein (bovine milk)
alpha-amylase (Bacillus)
xanthine oxidase (buttermilk)
Column(s):
Presto FF-C18 (75 x 4.6mm)

Protein separation on non-porous ODS column

Application #:
TI0636
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
alpha-lactalbumin (bovine milk)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for alpha-lactalbumin separation

Application #:
TI0635
Type:
Application Note
Categories:
Column Performance
Proteins
Botanicals
Compounds:
cytochrome c (horse heart)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for cytochrome c separation

Application #:
TI0634
Type:
Application Note
Categories:
Column Performance
Proteins
Biochemistry/Metabolism
Compounds:
thyroglobulin( porcin thyroid)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for thyroglobulin separation

Application #:
TI0633
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
lectin (concanavalin A)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for lectin separation

Application #:
TI0632
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
albumin(chicken egg)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for ovalbumin separation

Application #:
TI0631
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
albumin(bovine serum)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for BSA separation

Application #:
TI0630
Type:
Application Note
Categories:
Column Performance
Proteins
Biochemistry/Metabolism
Compounds:
insulin
Column(s):
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope for insulin separation

Application #:
TI0629
Type:
Application Note
Categories:
Column Performance
Biochemistry/Metabolism
Amino acids and peptides
Compounds:
angiotensin II
Column(s):
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope for angiotensin II serparation

Application #:
TI0627
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
abumin (human serum)
Column(s):
Presto FF-C18 (50 x 4.6mm)
Presto FF-C18 (250 x 4.6mm)

The effect of gradient slope and column length for HSA serpation

Application #:
TI0626
Type:
Application Note
Categories:
Column Performance
Proteins
Compounds:
abumin (human serum)
Column(s):
Presto FF-C18 (250 x 4.6mm)

Gradient mixer volume affecting HSA separation

Application #:
TI0502
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Column Performance
Compounds:
phenyltrimethylammnonium
benzenesulfonic acid
benzamide
Column(s):
Scherzo SM-C18 (150 x 3.0mm)

The Scherzo SM-C18 column shows excellent batch to batch reproducibility for anionic, cationic, and neutral compounds.

Application #:
TI0500
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Steroids
Column Performance
Compounds:
acetaminophen
1-hydroxyN 7-azabenzotriazole
prednisolone
methyl 3-aminoS 2-thiophenecarboxylate
6-alphamethylprednisolone
corticosterone
4-aminobenzophenone
propylparaben
Column(s):
Scherzo SM-C18 (75 x 4.6mm)

The Scherzo SM-C18 column shows excellent batch to batch reproducibility for these small polar molecules.

Application #:
TI0476
Type:
Application Note
Categories:
Column Performance
Compounds:
1-hydroxy-7-azabenzotriazole
acetoaminophen
prednisolone
6-methylprednisolone
methyl-3-amino-2-thiophenecarboxylate
corticosterone
4-aminobenzophenone
propyl paraben
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CW-C18 (150 x 4.6mm)

Substitution of organic solvent for reversed-phase

Application #:
TI0475
Type:
Application Note
Categories:
Column Performance
Compounds:
1-hydroxy-7azabenzotriazole
acetoaminophen
prednisolone
6-methylprednisolone
methyl-3-amino-2-thiophenecarboxylate
corticosterone
4-aminobenzophenone
propyl paraben.
Column(s):
Cadenza CD-C18 (150 x 3.0mm)
Cadenza 5CD-C18 (150 x 4.6mm)
Cadenza CW-C18 (150 x 3.0mm)

Reducing organic solvent consumption

Application #:
TI0450
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Column Performance
Compounds:
propranolol
Column(s):
Unison US-C18 (150 x 4.6mm)

Propranolol

Application #:
TI0418
Type:
Application Note
Categories:
Column Performance
Compounds:
Tetracaine
Propylbenzoate
Column(s):
Cadenza CW-C18 (0 x 0mm)

pH Durability of Cadenza CW-C18

Application #:
TI0414
Type:
Application Note
Categories:
Column Performance
Compounds:
propylparaben
prednisolone
methyl 3-amino-2-thiophenecarboxylate
corticosterone
acetaminophen
6-alpha-methylprednisolone
4-aminobenzophenone
1-hydroxy-7-azabenzotriazole
Column(s):
Cadenza CW-C18 (150 x 4.6mm)

Batch-to-batch reproducibility of CW-C18

Application #:
TI0401
Type:
Application Note
Categories:
Column Performance
Compounds:
Phenylalkanes
Column(s):
Cadenza CD-C18 (0 x 0mm)
Cadenza CW-C18 (0 x 0mm)
Unison UK-C8 (0 x 0mm)
Unison UK-Phenyl (0 x 0mm)

Retention and hydrophobic properties of Cadenza CW-C18

Application #:
TI0400
Type:
Application Note
Categories:
Column Performance
Compounds:
triphenylene
phenol
o-terphenyl
caffeine
butylbenzene
amylbenzene
Column(s):
Cadenza CW-C18 (0 x 0mm)

Basic Interaction of Cadenza CW-C18

Application #:
TI0385
Type:
Application Note
Categories:
Column Performance
Compounds:
uracil
toluene
naphthalene
fluorene
benzene
Column(s):
Cadenza CW-C18 (150 x 2.0mm)

HT performance with wide-pore ODS phase

Application #:
TI0318
Type:
Application Note
Categories:
Column Performance
Compounds:
uridine
thymidine
propylbenzoate
benzoic acid
3-hydroxyphenylurea
Column(s):
Unison UK-Amino (150 x 2.0mm)

Unison UK-Amino batch to batch reproducibility

Application #:
TI0316
Type:
Application Note
Categories:
Column Performance
Compounds:
L-ascorbic acid (Vitamin C)
isoascorbic acid (erythorbic acid)
Column(s):
Unison UK-Amino (250 x 2.0mm)

Normal phase and anion exchange mode

Application #:
TI0304
Type:
Application Note
Categories:
Saccharides
Column Performance
Compounds:
sucrose
maltose
glucose
frutose
Column(s):
Unison UK-Amino (250 x 2.0mm)

Unison UK-Amino aqueous durability

Application #:
TI0301
Type:
Application Note
Categories:
Industrial Chemicals
Column Performance
Compounds:
technical seperation of anion surfactants that do not recognize linear chain or multi-branch
Column(s):
Unison UK-Phenyl (150 x 4.6mm)

Anion surfactants

Application #:
TI0300
Type:
Application Note
Categories:
Industrial Chemicals
Column Performance
Compounds:
Aniomic Surfactants (Alkylbensene sulfonates)
Column(s):
Cadenza CD-C18 (250 x 4.6mm)

Anion surfactants

Application #:
TI0290
Type:
Application Note
Categories:
Proteins
Column Performance
Compounds:
xanthine oxidase
thyroglobulin
papain
lysozyme
lactferrin
insulin
hemoglobin (human)
hemoglobin (bovine)
deoxyribonclease
cytochrome ( c )
beta-glucosidase
apha-lactalbumin
angiotension II
alpha-chymotryosinogen (bovine pancreas)
albumin (human serum)
albumin (eggs)
albumin (bovine)
Column(s):
Cadenza HS-C18 (150 x 4.6mm)

Protein exclusion on Cadenza HS-C18

Application #:
TI0276
Type:
Application Note
Categories:
Pharmaceuticals & Cosmetics
Column Performance
Compounds:
sulfisoxazole
sulfathiazole
sulfaquinoxaline
sulfamonomethoxine
sulfamethoxazole
sulfamethaxine
sulfamerazine
sulfadiazine
Column(s):
Cadenza CD-C18 (20 x 2.0mm)

HT sulfonamides

Application #:
TI0266
Type:
Application Note
Categories:
Column Performance
Industrial Chemicals
Environmental and Agricultural
Compounds:
o-methoxyphenol
m-methoxyphenol
p-methoxyphenol
Column(s):
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CL-C18 (150 x 4.6mm)

Positional Isomers on CL-C18

Application #:
TI0265
Type:
Application Note
Categories:
Column Performance
Environmental and Agricultural
Industrial Chemicals
Compounds:
biphenyl
o-terphenyl
m-terphenyl
triphenylene
p-terphenyl
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)

Separation Properties of Polycyclic Aromatic Compounds on CL-C18

Application #:
TI0254
Type:
Application Note
Categories:
Column Performance
Steroids
Compounds:
propylbenzoate
deoxycorticosterone acetate
progesterone
butylbenzoate
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)

Separation properties for Neutral Compounds on CL-C18

Application #:
TI0253
Type:
Application Note
Categories:
Column Performance
Compounds:
thiabendazole
o-phenylphenol
imazalil
diphenyl
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)

Electrostatic Interaction on CL-C18

Application #:
TI0252
Type:
Application Note
Categories:
Column Performance
Compounds:
Triphenylene
Phenol
o-Terphenyl
Caffeine
Butylbenzene
Amylbenzene
Column(s):
Cadenza CD-C18 (0 x 0mm)
Cadenza CL-C18 (0 x 0mm)
Unison UK-C18 (0 x 0mm)

Basic interaction of CL-C18, CD-C18, and UK-C18

Application #:
TI0251
Type:
Application Note
Categories:
Column Performance
Compounds:
propylbenzoate
Column(s):
Cadenza CL-C18 (0 x 0mm)

pH durability of CL-C18

Application #:
TI0250
Type:
Application Note
Categories:
Column Performance
Compounds:
Propylparaben
Prednisolone
methyl 3-amino-2-thiophenecarboxylate
Corticosterone
Acetaminophen
6-alpha-methylprednisolone
4-aminobenzophenone
1hydroxy-7-azabenzotriazole
Column(s):
Cadenza CL-C18 (75 x 4.6mm)

Batch to batch reproduciblity of CL-C18

Application #:
TI0247
Type:
Application Note
Categories:
Column Performance
Pharmaceuticals & Cosmetics
Biochemistry / Metabolism
Compounds:
procainamide
N-acetylprocainamide
Column(s):
Cadenza CD-C18 (30 x 4.6mm)
Cadenza CL-C18 (30 x 4.6mm)

Separation properties of basic compounds on CL-C18

Application #:
TI0246
Type:
Application Note
Categories:
Column Performance
Biochemistry / Metabolism
Food Chemistry
Pharmaceuticals & Cosmetics
Compounds:
benzoic acid
salicylic acid
theophylline
phenol
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CL-C18 (75 x 4.6mm)

Separation properties for acidic compounds on CL-C18

Application #:
TI0245
Type:
Application Note
Categories:
Column Performance
Biochemistry / Metabolism
Compounds:
theophylline
phenol
caffeine
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

Retention behavior of polar compounds

Application #:
TI0203
Type:
Application Note
Categories:
Utilizing a high-throughput column for enzymatic reaction analysis
Compounds:
p-nitorophenyl-beta-glucoside
p-nitrophenol
Column(s):
Unison UK-C18 (10 x 2.0mm)

Analysis of enzymatic reaction using a high throughput 10 mm column.

Application #:
TI0202
Type:
Application Note
Categories:
Compounds:
alkyl parabens
Column(s):
Unison UK-C18 (10 x 2.0mm)

Our 10 mm long column can be used instead of a conventional 20-35mm column, performing high-speed separation in approximately 20 seconds while reducing column length. Furthermore our 10mm column has an impressive lifetime of over 2000 injections.

Application #:
TI0200
Type:
Application Note
Categories:
Out of elution order by electrostatic interaction
Compounds:
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

The effect of electrostatic interaction on retention. Hydrophobic interaction, which depends on hydrogen bonding capacity and dipole-dipole interaction, affects the retention time of analysis on different columns.

Application #:
TI0186
Type:
Application Note
Categories:
Loading Capacity of Preparative Column
Compounds:
econazole nitrate
miconazole nitrate
Column(s):
Unison US-C18 (250 x 20.0mm)
Unison US-C18 (250 x 4.6mm)

Loading capacity of our versatile 20mm column is demonstrated at the laboratory level.

Application #:
TI0150
Type:
Application Note
Categories:
High-efficiency separation of surfactants by a 100
000 plate column
Compounds:
Triton X-100
Column(s):
Cadenza CD-C18 (500 x 4.6mm)

The 500x4.6mm column shows marked separation efficiency in comparison with the 5um ODS column.

Application #:
TI0149
Type:
Application Note
Categories:
Relationship between Flow Rate and Theoretical Plates on a 100
000 Plates Column
Compounds:
alkylparabens
Column(s):
Cadenza CD-C18 (500 x 4.6mm)

The 500x4.6mm column delivers a high number of theoretical plates at a low flow rate.

Application #:
TI0148
Type:
Application Note
Categories:
Effects of Flow Rate and Eluent on a 100
000 Plates Column
Compounds:
methyl paraben
Column(s):
Cadenza CD-C18 (500 x 4.6mm)

The 500x4.6mm column delivers a high number of theoretical plates under moderate pressure.

Application #:
TI0147
Type:
Application Note
Categories:
Efficiency of 500x4.6mm ODS Column
Compounds:
alkylparabens
Column(s):
Cadenza CD-C18 (500 x 4.6mm)

The 500x4.6mm column performs with equal or greater efficiency than that of two connected 250mm columns.

Application #:
TI0126
Type:
Application Note
Categories:
Manufacturing Batch-to-Batch Reproducibility (UK-Phenyl)
Compounds:
1-hydroxy-7-azabenzotriazole
acetaminophen
predonizolone
6-methylprednisolone
metyl-3-amino-2-thiophenecarboxylate
corticosterone
4-aminobenzophenone
propylparaben
Column(s):
Unison UK-Phenyl (75 x 4.6mm)

Demonstrates the batch-to-batch reproducibility of UK-Phenyl with 3um phenyl packing material in three trials.

Application #:
TI0125
Type:
Application Note
Categories:
Manufacturing Batch-to-Batch Reproducibility
Compounds:
1-hydroxy-7-azabenzotriazole
acetaminophen
predonizolone
Column(s):
Unison UK-C8 (75 x 4.6mm)

Demonstrates the batch-to-batch reproducibility of UK-C8 with 3um octyl packing material in three trials.

Application #:
TI0124
Type:
Application Note
Categories:
Column Performance
Compounds:
Propylparaben
Prednisolone
methyl 3-amino-2-thiophenecarboxylate
Corticosterone
Acetaminophen
6-alpha-methylprednisolone
4-aminobenzophenone
1hydroxy-7-azabenzotriazole
Column(s):
Unison UK-C18 (75 x 4.6mm)

Unison UK-C18 batch to batch reproducibility

Application #:
TI0123
Type:
Application Note
Categories:
Manufacturing Batch-to-Batch Reproducibility (US-C18
5um)
Compounds:
1-hydroxy-7-azabenzotriazole
acetaminophen
predonizolone
6-methylprednisolone
metyl-3-amino-2-thiophenecarboxylate
corticosterone
4-aminobenzophenone
propylparaben
Column(s):
Unison US-C18 (75 x 4.6mm)

Demonstrates batch-to-batch reproducibility of US-C18 with 5um ODS packing material in three trials.

Application #:
TI0118
Type:
Application Note
Categories:
pH Stability of Unison Series
Compounds:
propylbenzoate
Column(s):
Unison UK-C18 (75 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)
Unison UK-Phenyl (75 x 4.6mm)

Demonstrates the pH durability Unison stationary phase series (UK-C18, UK-C8, UK-Phenyl)

Application #:
TI0117
Type:
Application Note
Categories:
Phase Interaction
Compounds:
caffeine
phenol
butylbenzene
amylbenzene
o-terphenyl. triphenylene
Column(s):
Cadenza CD-C18 (0 x 0mm)
Unison UK-C18 (0 x 0mm)

Differences in ODS stationary phase interactions between Unison and Cadenza.

Application #:
TI0115
Type:
Application Note
Categories:
Column Performance
Biochemistry / Metabolism
Compounds:
Theophylline
phenol
caffeine
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

Retention properties of polar compounds

Application #:
TI0114
Type:
Application Note
Categories:
Retention Behavior on Unison and Cadenza
Compounds:
caffeine
butylparaben
diphenyl
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)

Comparison of elution quality between our high polar affinity ODS Unison column and high resolution Cadenza Column.

Application #:
TI0089
Type:
Application Note
Categories:
Column size comparison for elution of investigational medication
Compounds:
investigational medication
Column(s):
Cadenza CD-C18 (250 x 2.0mm)
Cadenza CD-C18 (250 x 3.0mm)

500 uL injection of eluent into a 2mm internal diameter column under optimal conditions.

Application #:
TI0088
Type:
Application Note
Categories:
Column performance in an aqueous elution
Compounds:
cytosine
uracil
cytidine
Column(s):
Unison UK-C18 (30 x 4.6mm)
Unison UK-C18 (50 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison UK-C18 (100 x 4.6mm)
Unison UK-C18 (150 x 4.6mm)
Unison UK-C18 (250 x 4.6mm)

Unison offers high performance in 100 percent aqueous elution, where conventional ODS columns lose retention.

Application #:
TI0087
Type:
Application Note
Categories:
Benefits of a phenyl column
Compounds:
o-toluidine
m-toluidine
p-toluidine
Column(s):
Unison UK-Phenyl (150 x 4.6mm)

Our phenyl column offers distinct selectivity in the separation of constitutional isomers when compared to conventional ODS columns.

Application #:
TI0086
Type:
Application Note
Categories:
Compounds:
nitrobenzene
benzene
ethylbenzene
toluene
naphthalene
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)

Our C8 column can offer more balanced high speed separation or different selectivity than can an ODS column.

Application #:
TI0075
Type:
Application Note
Categories:
Impurities separation for pharmaceutical compound
Compounds:
investigational medication
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

Cadenza produces peaks for difficult to separate impurities.

Application #:
TI0074
Type:
Application Note
Categories:
Column Durability in Gradient Analysis
Compounds:
N-acetylprocainamide
carbamazepine
o-phenylphenol
diphenyl
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Cadenza withstands over 3000 runs of gradient analysis under optimal conditions.

Application #:
TI0071
Type:
Application Note
Categories:
The relationship between plate numbers and flow rate for high-resolution columns
Compounds:
metylparaben
Column(s):
Cadenza CD-C18 (250 x 4.6mm)

Our 250 x 4.6 mm columns can obtain high theoretical plate counts at low flow rates with low back pressure.

Application #:
TI0068
Type:
Application Note
Categories:
Elution mode comparison of high-throughput separation
Compounds:
n-alkylparabens
Column(s):
Cadenza CD-C18 (30 x 3.0mm)

Comparison of elution types in super high-speed separations using 30 x 3.0 mm

Application #:
TI0067
Type:
Application Note
Categories:
High speed separation through small columns
Compounds:
theobromine
theophylline
caffeine
Column(s):
Cadenza CD-C18 (30 x 2.0mm)
Cadenza CD-C18 (30 x 3.0mm)
Cadenza CD-C18 (30 x 4.6mm)

Using our 30 mm column, analysis is possible in 30 seconds.

Application #:
TI0064
Type:
Application Note
Categories:
Column durability through repeat injections
Compounds:
uracil
butylparaben
Column(s):
Cadenza CD-C18 (250 x 4.6mm)

Our high resolution column shows durability after 5000 runs at optimal conditions.

Application #:
TI0062
Type:
Application Note
Categories:
pH stability in the stationary phase
Compounds:
Column(s):
Cadenza CD-C18 (0 x 0mm)

Cadenza CD-C18 demonstrates pH stability in comparision with conventional ODS columns.

Application #:
TI0058
Type:
Application Note
Categories:
The next-generation standard in high-throughput columns
Compounds:
alkylbenzoates
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Cadenza maintains its efficiency with highly sensitive high-speed analysis and low solvent use.

Application #:
TI0015
Type:
Application Note
Categories:
A
Compounds:
1-hydroxy-7-azabenzotriazole
acetaminophen
predonizolone
6-methylprednisolone
metyl-3-amino-2-thiophenecarboxylate
corticosterone
4-aminobenzophenone
propylparaben
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Cadenza CD-C18 (150 x 4.6mm)
Cadenza CD-C18 (250 x 4.6mm)

Cadenza's 3um particle size revolutionizes columns length, speed and separation efficiency.

Application #:
TI0008
Type:
Application Note
Categories:
Batch-to-batch reproducibility
Compounds:
1-hydroxy-7-azabenzotriazole
acetaminophen
predonizolone
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Cadenza CD C18 offers superior batch-to-batch reproducibility.

Application #:
SP0033
Type:
Poster
Categories:
LC-MS
Amino Acids and Peptides
Column Performance
Biochemistry/Metabolism
Compounds:
arg
lys
his
(cys)2
asn
gln
ser
gly
asp
glu
thr
ala
val
pro
tyr
met
leu
ile
trp
phe
aminoadipic acid
aminoethanol
carnosine
beta-aminoisobutyric acid
hydroxylisine
hydroxyproline
ornithine
beta-alanine
sarcosine
methionine sulfone
anserine
alpha-aminobutyric acid
methylhistidine
theanine
cystathionine
cysteine
citrulline
aminobutyric acid
taurine
nicotinamide
pyridoxine (B6)
roboflavin (B2)
nicotinic acid (B3)
beta-aminobutyric acid
beta-aminoisobutyric acid
gamma-aminobutyric acid
o-methylserine
L-threonine
allo-L-threonine
homoserine
1
2-dimyristoyl-sn-glycero-3-phosphocholine
1
2-dimyristoyl-sn-glycero-3-phosphoethanolamine
acetylcholine chloride
thrimethylglycine (betaine)
N
N-dimethylglycine
L-methionine
S-(5'-adenosyl)-L-homocysteine (SAH)
phosphocholine
choline chloride
cytidine 5'-diphosphocholine
S-(5'-adenosyl)-L-methionine (SAM)
Column(s):
Intrada Amino Acid (50 x 3.0mm)
Intrada Amino Acid (100 x 3.0mm)
Intrada Amino Acid (150 x 3.0mm)
Intrada Amino Acid (250 x 3.0mm)

The world's first specialty HPLC column for the analysis of amino acids without derivatization using LC-MS

Application #:
SP0032
Type:
Poster
Categories:
LC-MS
Amino Acids and Peptides
Column Performance
Biochemistry/Metabolism
Compounds:
arginine
ornithine
hydroxylysine
lysine
carnosine
anserine
1-methylhistidine
3-methylhistidine
histidine
2-aminoethanol
cystathionine
5-aminolevulinic acid
cystine
alpha-aminobutyric acid
beta-aminobutyric acid
beta-aminoisobutyric acid
gama-aminobutyric acid
o-phosphoethanolamine
5-aminovaleric acid
glycylproline
o-phosphoserin
citrulline
asparagine
glycine
glutamine
serine
alanine
sarcosine
beta-alanine
aspartic acid
threonine
o-methylserine
allo-threonine
homoserine
hydroxyproline
proline
taurine
methionine sulfone
glutamic acid
alpha-aminoadipic acid
theanine
cysteine
norvaline
valine
alpha-aminopimelic acid
leucine
isoleucine
allo-isoleucine
norleucine
methionine
tyrosine
phenylalanine
tryptophan
thioproline
cysteic acid
5-hydroxy-L-tryptophan
3
4-dihydroxy-L-phenylalanine
5-hydroxyindole-3-acetic acid
dopamine
L-noradrenaline
serotonin
acetylcholine chloride
L-adrenaline
phenethylamine
tyramine
tryptamine
histamine
putresine
cadaverine
agmatine
3-methoxytyramine
3
4-dihydroxyphenyl acetic acid
homovanillic acid
L-carnitine
L-homocystine
L-homocysteine
S-(5'-adenosyl)-L-methionine (SAM)
S-(5'-adenosyl)-L-homocysteine (SAH)
L-kynurenine
kynurenic acid
3-hydroxyanthranilic acid
picolinic acid
quinolinic acid
carnitine
Column(s):
Intrada Amino Acid (10 x 2.0mm)
Intrada Amino Acid (10 x 3.0mm)
Intrada Amino Acid (50 x 3.0mm)
Intrada Amino Acid (100 x 3.0mm)
Intrada Amino Acid (150 x 3.0mm)

Amino acids and metabolites/derivatives analysis without derivatization using a novel mixed-mode column

Application #:
SP0028
Type:
Poster
Categories:
Biochemistry/Metabolism
Column Performance
Pharmaceuticals and Cosmetics
Amino Acids and Peptides
Compounds:
nicotinic acid
tryptophan
bromoguanosine
labetalol
aspirin
fexofenadine
reserpine
SB243213A
ibuprofen
penicillin V
Column(s):

An Evaluation of a Multi-Mode Stationary Phase for HPLC Separations Using a Multi-Component Test Mix

Application #:
SP0026
Type:
Poster
Categories:
LC-MS
Pharmaceuticals and Cosmetics
Column Performance
Compounds:
verapamil
reserpine
propranolol
Column(s):

Evaluation of Column Retentivity with large injection volume for high sensitive and High-Throughput LC-MS/MS Quantitative Analysis

Application #:
SP0022
Type:
Poster
Categories:
0
Compounds:
0
Column(s):
Intrada Amino Acid (50 x 3.0mm)

Simultaneous quantitative analysis of 20 amino acids in food samples without derivatization using LC - MS/MS

Application #:
SP0021
Type:
Poster
Categories:
0
Compounds:
0
Column(s):
Intrada Amino Acid (10 x 2.0mm)
Intrada Amino Acid (50 x 3.0mm)
Intrada Amino Acid (75 x 2.0mm)
Intrada Amino Acid (100 x 3.0mm)
Intrada Amino Acid (150 x 2.0mm)
Intrada Amino Acid (150 x 3.0mm)
Scherzo SS-C18 (150 x 3.0mm)
Unison UK-C18 (250 x 4.6mm)
Unison UK-Amino (250 x 2.0mm)

The world's first specialty column for intact amino acid analysis via LC-MS

About Imtakt

Our HPLC columns have been designed and manufactured by Imtakt Corporation with exceptional craftsmanship and decades of industry experience. Our columns give chromatographers high resolution for unique problems.

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