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Technical Information Posters Papers
LC-MS
cGMP
AMP
GMP
LC-MS: Cyclic Mononucleotides
Biochemistry/Metabolism
Oligonucleotides (Oligothymidylic acid)
Biochemistry/Metabolism
LC-MS
pseudouridine
LC-MS: Uridine isomer (psi-uridine)
Biochemistry/Metabolism
LC-MS
ATP
dGTP
UTP
CTP
GTP
dATP
dCTP
LC-MS: Nucleoside triphosphate (NTP)
Biochemistry/Metabolism
LC-MS
ATP
dGTP
UTP
CTP
GTP
dATP
dCTP
LC-MS: Nucleoside triphosphate (NTP)
DNA Related Compounds
Biochemistry/Metabolism
LC-MS
1-methylimidazole
4-methylimidazole
LC-MS: 4-Methylmidazole in Cola and Instant Coffee
DNA Related Compounds
Biochemistry/Metabolism
LC-MS
1-methylimidazole
4-methylimidazole
LC-MS: 4-Methylmidazole in Dark Beer
Industrial Chemical
DNA Related Compounds
Biochemistry/Metabolism
LC-MS
2-methylimidazole
1-methylimidazole
4-methylimidazole
LC-MS: Methylmidazole isomers
LC-MS
5'-GGAAACAGCTATGACCATGA-3'
LC-MS: Deoxyoligonucleotides
LC-MS
5'-GGAAACAGCTATGACCATGA-3'
LC-MS: Deoxyoligonucleotides
LC-MS
5'-GGAAACAGCTATGACCATGA-3'
LC-MS: Deoxyoligonucleotides
LC-MS
LC-MS: Oligonucleotides (Oligothymidylic acid)
LC-MS
LC-MS: Oligonucleotides (Oligothymidylic acid)
LC-MS
LC-MS: Oligonucleotides (Oligothymidylic acid)
Biochemistry/Metabolism
LC-MS
NADH
NADP
NADPH
LC-MS: Coenzymes (Nicotinamide adenine dinucleotides)
DNA Related Compounds
Acid stability of Unison UK-C1
DNA Related Compounds
Biochemistry/Metabolism
SEC: Ribonuclease A (RNase A), from bovine pancreas
SEC: 20bp DNA Ladder
DNA Related Compounds
thymidine
adenine
adenosine
uracil
uridine
cytosine
cytidine
guanine
guanosine
LC-MS: Nucleosides and bases (mobile phase pH)
DNA Related Compounds
thymidine
adenine
adenosine
uracil
uridine
cytosine
cytidine
guanine
guanosine
LC-MS: Nucleosides and bases
DNA related compounds
Column Performance
isopropylparaben
propylparaben
butylparaben
The Cadenza CD-C18 HT 3 micron column shows comparable resolution to a sub-2 micron column, with much lower pressure.
DNA related compounds
Column Performance
isopropylparaben
propylparaben
butylparaben
The Cadenza CD-C18 HT 3 micron column shows better resolution between isopropylparaben and propylparaben than a sub-2 micron column, and the CD-C18 HT column also shows a higher plate count.
DNA related compounds
Column Performance
isopropylparaben
propylparaben
butylparaben
The Cadenza CD-C18 HT 3 micron column shows lower pressure, higher plate count, and higher resolution than a sub-2 micron column.
Oligonucleotides
Control siRNA
Negative Control
Control siRNA duplex (Human cdk1)
Control siRNA duplex (Human beta-actin)
siRNA
Ribonucleic acid (RNA)
Double-stranded DNA fragments
Plasmid DNA
DNA from salmon spermary
Biochemistry / Metabolism
adenosine 5'- monophosphate disodium (AMP)
adenosine 5'- diphosphate disodium (ADP)
Adenosine Phosphates (AMP, ADP, ATP)
Cadenza CW-C18 and Cadenza CL-C18 have different selectivities for double-stranded DNA. CW-C18 is a wide pore column that elutes the molecules earlier than the CL-C18, which is selective via its exposed silanol groups.
Unison UK-Amino separates mononucleotides of similar structure. For example, dGMP and GMP are resolved even though they differ in only a single hydroxyl group.
adenine
guanine
cytosine
Separation of highly polar nucleic acid bases in normal phase mode due to high organic solvent density.
adenosine
guanosine
cytidine
Aqueous normal phase separation of nucleosides using a silica column.
uracil
cytidine
guanine
uridine
thymine
adenine
guanosine
thymidine
In comparison with the 500mm column, our 3um 250mm separates with high efficiency.
inosine
inosine 5'-monophosphate (IMP)
adenosine 5'-monophosphate (AMP)
adenosine 5'-diphosphate (ADP)
adenosine 5'-triphosphate (ATP)
Analysis of purine nucleotides under LC-MS conditions.
d(T)12-18
Comparison of internal diameter in the reverse phase separation of oligo nucleotides.
uracil
guanine
thymine
adenine
Comparison of 5um column separation of nucleic acid bases. Unison US-C18 demonstrates excellent performance.
Comparative elution between stationary phases in ammonium acetate.
inosine
inosine 5'-monophosphate (IMP)
adenosine 5'-monophosphate (AMP)
adenosine 5'-diphosphate (ADP)
adenosine 5'-triphosphate
Separation of nucleotides used to rate freshness in seafood.
inosine
inosine 5'-monophosphate (IMP)
Separation of inosinic acid, a flavor enhancer.
ADP
ATP
High-speed separation of adenosine nucleotides.