LC-MS/MS: Isomer separation of central metabolites using the multi-modal properties of a hybrid column

Application Code: TID70E

"LC-MS/MS: Isomer separation of central metabolites using the multi-modal properties of a hybrid column" is an HPLC analysis example presented by Imtakt. This page provides an overview of HPLC application data related to Metabolite, BioCompd from Imtakt, an HPLC column manufacturer.This application provides information on the compounds analyzed, columns used, analytical conditions, detection conditions, and chromatograms. This information serves as a reference for HPLC method development, evaluation of separation conditions, and column selection.For detailed analytical conditions and technical information, please refer to the PDF at the bottom of this page.

📄 Application note

HPLC analysis example of LC-MS/MS: Isomer separation of central metabolites using the multi-modal properties of a hybrid column.
Please refer to the PDF for detailed analytical conditions and chromatograms.

Download PDF (TID70E.pdf)

📋 General Information

Application CodeTID70E
TitleLC-MS/MS: Isomer separation of central metabolites using the multi-modal properties of a hybrid column
CategoryMetabolite, BioCompd
Compounds
2-phosphoglyceric acid (2PGA)3-phosphoglyceric acid (3PGA)citric aciddihydroxyacetone phosphate (DHAP)glucose 1-phosphate (G1P)glucose 6-phosphate (G6P)glyceraldehyde 3-phosphate (G3P)isocitric acidribose 5-phosphate (R5P)ribulose 5-phosphate (Ru5P)sucrose 6-phosphate (S6P)trehalose 6-phosphate (T6P)

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