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Technical Information   Posters   Papers

Application #:
TI0867
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
20bp DNA Ladder
Column(s):
(250 x 4.6mm)

SEC: 20bp DNA Ladder

Application #:
TI0844
Type:
Application Note
Categories:
LC-MS
DNA Related Compounds
Compounds:
thymine
thymidine
adenine
adenosine
uracil
uridine
cytosine
cytidine
guanine
guanosine
Column(s):

LC-MS: Nucleosides and bases (mobile phase pH)

Application #:
TI0840
Type:
Application Note
Categories:
LC-MS
DNA Related Compounds
Compounds:
thymine
thymidine
adenine
adenosine
uracil
uridine
cytosine
cytidine
guanine
guanosine
Column(s):
Unison UK-Amino (150 x 3.0mm)

LC-MS: Nucleosides and bases

Application #:
TI0709
Type:
Application Note
Categories:
Pharmaceutical and Cosmetics
DNA related compounds
Column Performance
Compounds:
uracil
isopropylparaben
propylparaben
butylparaben
Column(s):
Cadenza CD-C18 (100 x 4.6mm)

The Cadenza CD-C18 HT 3 micron column shows comparable resolution to a sub-2 micron column, with much lower pressure.

Application #:
TI0708
Type:
Application Note
Categories:
Pharmaceutical and Cosmetics
DNA related compounds
Column Performance
Compounds:
uracil
isopropylparaben
propylparaben
butylparaben
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

The Cadenza CD-C18 HT 3 micron column shows better resolution between isopropylparaben and propylparaben than a sub-2 micron column, and the CD-C18 HT column also shows a higher plate count.

Application #:
TI0707
Type:
Application Note
Categories:
Pharmaceuticals and Cosmetics
DNA related compounds
Column Performance
Compounds:
uracil
isopropylparaben
propylparaben
butylparaben
Column(s):
Cadenza CD-C18 (150 x 4.6mm)

The Cadenza CD-C18 HT 3 micron column shows lower pressure, higher plate count, and higher resolution than a sub-2 micron column.

Application #:
TI0680
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
oligothymidylic acid d(pT) ammonium salt
Column(s):
Scherzo SW-C18 (150 x 3.0mm)

Oligonucleotides

Application #:
TI0673
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
siRNA Universal Negative Control
Control siRNA
Negative Control
Control siRNA duplex (Human cdk1)
Control siRNA duplex (Human beta-actin)
Column(s):
Scherzo SW-C18 (150 x 3.0mm)

siRNA

Application #:
TI0587
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
ribonucleic acid (from yeast)
Column(s):
Presto FF-C18 (30 x 4.6mm)
Presto FF-C18 (150 x 4.6mm)

Ribonucleic acid (RNA)

Application #:
TI0576
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
salmon DNA / DNase
Column(s):
Presto FF-C18 (250 x 4.6mm)

Double-stranded DNA fragments

Application #:
TI0558
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
pUC18 (2
686 bp) pBR322 (4
361 bp)
Column(s):
Presto FF-C18 (250 x 4.6mm)

Plasmid DNA

Application #:
TI0557
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
DNA
Column(s):
Presto FF-C18 (150 x 4.6mm)

DNA from salmon spermary

Application #:
TI0448
Type:
Application Note
Categories:
DNA Related Compounds
Biochemistry / Metabolism
Compounds:
adenosine 5'- triphosphate disodium (ATP)
adenosine 5'- monophosphate disodium (AMP)
adenosine 5'- diphosphate disodium (ADP)
Column(s):
Unison UK-Amino (30 x 3.0mm)

Adenosine Phosphates (AMP, ADP, ATP)

Application #:
TI0366
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
Double-Stranded DNA Fragments
Column(s):
Cadenza CW-C18 (250 x 4.6mm)
Cadenza CL-C18 (250 x 4.6mm)

Cadenza CW-C18 and Cadenza CL-C18 have different selectivities for double-stranded DNA. CW-C18 is a wide pore column that elutes the molecules earlier than the CL-C18, which is selective via its exposed silanol groups.

Application #:
TI0363
Type:
Application Note
Categories:
DNA Related Compounds
Compounds:
mononucleotides
Column(s):
Unison UK-Amino (250 x 4.6mm)

Unison UK-Amino separates mononucleotides of similar structure. For example, dGMP and GMP are resolved even though they differ in only a single hydroxyl group.

Application #:
TI0171
Type:
Application Note
Categories:
Nucleic Acid Bases
Compounds:
thymine
adenine
guanine
cytosine
Column(s):
Unison UK-Silica (250 x 2.0mm)

Separation of highly polar nucleic acid bases in normal phase mode due to high organic solvent density.

Application #:
TI0167
Type:
Application Note
Categories:
Separation of high polarity compounds with a 3um silica column
Compounds:
thymidine
adenosine
guanosine
cytidine
Column(s):
Unison UK-Silica (250 x 2.0mm)

Aqueous normal phase separation of nucleosides using a silica column.

Application #:
TI0152
Type:
Application Note
Categories:
Nucleoside and Bases
Compounds:
cytosine
uracil
cytidine
guanine
uridine
thymine
adenine
guanosine
thymidine
Column(s):
Unison UK-C18 (250 x 4.6mm)
Unison UK-C18 (500 x 4.6mm)

In comparison with the 500mm column, our 3um 250mm separates with high efficiency.

Application #:
TI0133
Type:
Application Note
Categories:
Purine Nucleotides
Compounds:
hypoxanthine
inosine
inosine 5'-monophosphate (IMP)
adenosine 5'-monophosphate (AMP)
adenosine 5'-diphosphate (ADP)
adenosine 5'-triphosphate (ATP)
Column(s):
Unison UK-C18 (75 x 4.6mm)

Analysis of purine nucleotides under LC-MS conditions.

Application #:
TI0116
Type:
Application Note
Categories:
Oligonucleotides
Compounds:
deoxy thimidine oligonucleotides
d(T)12-18
Column(s):
Unison US-C18 (150 x 2.0mm)
Unison US-C18 (150 x 3.0mm)
Unison US-C18 (150 x 4.6mm)

Comparison of internal diameter in the reverse phase separation of oligo nucleotides.

Application #:
TI0108
Type:
Application Note
Categories:
Nucleic Acid Bases
Compounds:
cytosine
uracil
guanine
thymine
adenine
Column(s):
Unison US-C18 (150 x 4.6mm)

Comparison of 5um column separation of nucleic acid bases. Unison US-C18 demonstrates excellent performance.

Application #:
TI0106
Type:
Application Note
Categories:
Oligonucleotides
Compounds:
d(T)12-18
Column(s):
Cadenza CD-C18 (75 x 4.6mm)
Unison UK-C18 (75 x 4.6mm)
Unison UK-C8 (75 x 4.6mm)

Comparative elution between stationary phases in ammonium acetate.

Application #:
TI0084
Type:
Application Note
Categories:
Purine Nucleotides
Compounds:
hypoxanthine
inosine
inosine 5'-monophosphate (IMP)
adenosine 5'-monophosphate (AMP)
adenosine 5'-diphosphate (ADP)
adenosine 5'-triphosphate
Column(s):
Unison US-C18 (150 x 4.6mm)

Separation of nucleotides used to rate freshness in seafood.

Application #:
TI0047
Type:
Application Note
Categories:
Inosinic acid and metabolites
Compounds:
hypoxanthine
inosine
inosine 5'-monophosphate (IMP)
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

Separation of inosinic acid, a flavor enhancer.

Application #:
TI0032
Type:
Application Note
Categories:
Adenosine Nucleotides
Compounds:
AMP
ADP
ATP
Column(s):
Cadenza CD-C18 (75 x 4.6mm)

High-speed separation of adenosine nucleotides.

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