The HPLC analysis of oligonucleotides is emerging in the study of DNA related compounds. Due to the highly charged phosphate groups on the oligonucleotide molecule, HPLC separation is difficult on conventional C18 HPLC columns. The Scherzo SW-C18 column contains a small amount of strong ionic ligands and reverse phase ligands, allowing it to effectively elute highly charged oligonucleotide molecules. As shown in the link below, the Scherzo SW-C18 HPLC column can also recognize the different structures and molecular weights of the varying oligonucleotide lengths.
Oligonucleotide data:
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Small polar molecules such as neurotransmitters are difficult to retain on conventional C18 columns. As shown in the link below, the Scherzo SS-C18 HPLC column (containing a large amount of strong ionic ligands and reverse phase ligands) successfully retains a variety of neurotransmitters. Scherzo SS-C18 also retains small, polar histamine related compounds (see link below). Not only can this unique multi-mode HPLC column successfully retain small molecules, it can also recognize slight structural differences. Histamine and N-methylhistamine differ only in one methyl group, but are clearly resolved into two separate peaks. L-noradrenaline and dopamine differ in only one hydroxyl group and are also successfully separated by Scherzo SS-C18.
Neurotransmitter data:
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Histamine related compounds data:
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