The demand for ultra-fast analysis of drug compounds is increasing in the pharmaceutical market. Sub-2 micron HPLC columns are not the only type of columns that can achieve ultra-fast run times, ultra-high pressure limits, and high flow rates. Imtakt Corporation has developed 3 micron high throughput columns that can analyze drug compounds in a matter of seconds. As shown in the link below, the Cadenza CD-C18 HT column separates nifedipine, warfarin, diclofenac, and 2-amino-5-chlorobenzophenone in 9.58 seconds. In the below application the flow rate (3.0 mL/min) was 5x the maximum flow rate for a standard Cadenza CD-C18 column, and max pressure (400 bar) was 1.6x the maximum pressure for a standard Cadenza CD-C18 column. Moreover, the short run time did not compromise the resolution of the four drug compounds.
Cadenza CD-C18 HT data:
http://www.imtaktusa.com/site_media/files/technical_information/TI676E.pdf
For columns we have available in HT and UP, as well as max pressure limits, see link below:
http://www.imtaktusa.com/site_media/files/phase_brochures/HT_and_UP.pdf
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Compounds of similar structure are difficult to separate and sometimes coelute on C18 columns. Recently, our Cadenza CD-C18 HPLC column was able to separate variations of sphingomyelin differing only in carbon chain length and degree of unsaturation. As shown in the link below, compounds 3 and 4, differing only in one double bond, were clearly resolved with this column. This type of sensitive molecular recognition/separation is very useful for scientists who are studying structural isomers.
Sphingomyelin data:
http://www.imtaktusa.com/site_media/files/technical_information/TI512E.pdf
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Small polar compounds are usually hard to retain on conventional C18 HPLC columns. The Scherzo family columns have novel multimode technology that produces excellent retention of serotonin related compounds. The Scherzo SM-C18 column contains weak ionic ligands in combination with reverse phase ligands. The Scherzo SW-C18 column contains a small amount of strong ionic ligands in combination with reverse phase ligands, and the Scherzo SS-C18 column contains a large amount of strong ionic ligands in combination with reverse phase ligands. The difference in the strengths of the ionic ligands results in differing retention of serotonin. As shown in the link below, serotonin (peak 2) is least retained on Scherzo SM-C18, more retained on Scherzo SW-C18, and best retained on Scherzo SS-C18.
Serotonin data:
http://www.imtaktusa.com/site_media/files/technical_information/TI683E.pdf
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