EC is the conventional method for polysaccharide analysis. A new approach uses reversed-phase with Presto FF-C18 (2um non-porous ODS). This method should be useful for quantitation of polysaccharides:
Proteoglycan:
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Fucoidan:
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Presto FF-C18 consists of C18 bonded to monodispersed 2um non-porous silica. It is useful for both biopolymer and synthetic polymer separations, gets very high efficiency with low flow rates, is compatible with conventional HPLC systems, and offers different selectivity. |
Researchers at Indiana University recently developed an LC-MS method for monosaccharide analysis:
LC-MS monosaccharides:
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Ref): Rapid Communications in Mass Spectrometry. Vol 24. Issue 11. (June 2010). 1565-1574 |
